PMID- 37895019
OWN - NLM
STAT- MEDLINE
DCOM- 20231030
LR  - 20240210
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 20
DP  - 2023 Oct 19
TI  - Cultured Mesenchymal Cells from Nasal Turbinate as a Cellular Model of the 
      Neurodevelopmental Component of Schizophrenia Etiology.
LID - 10.3390/ijms242015339 [doi]
LID - 15339
AB  - The study of neurodevelopmental molecular mechanisms in schizophrenia requires 
      the development of adequate biological models such as patient-derived cells and 
      their derivatives. We previously utilized cell lines with neural progenitor 
      properties (CNON) derived from the superior or middle turbinates of patients with 
      schizophrenia and control groups to study schizophrenia-specific gene expression. 
      In this study, we analyzed single-cell RNA seq data from two CNON cell lines (one 
      derived from an individual with schizophrenia (SCZ) and the other from a control 
      group) and two biopsy samples from the middle turbinate (MT) (also from an 
      individual with SCZ and a control). We compared our data with previously 
      published data regarding the olfactory neuroepithelium and demonstrated that CNON 
      originated from a single cell type present both in middle turbinate and the 
      olfactory neuroepithelium and expressed in multiple markers of mesenchymal cells. 
      To define the relatedness of CNON to the developing human brain, we also compared 
      CNON datasets with scRNA-seq data derived from an embryonic brain and found that 
      the expression profile of the CNON closely matched the expression profile one of 
      the cell types in the embryonic brain. Finally, we evaluated the differences 
      between SCZ and control samples to assess the utility and potential benefits of 
      using CNON single-cell RNA seq to study the etiology of schizophrenia.
FAU - Tung, Victoria Sook Keng
AU  - Tung VSK
AUID- ORCID: 0009-0007-5650-897X
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY 11203, USA.
FAU - Mathews, Fasil
AU  - Mathews F
AD  - Department of Otolaryngology, State University of New York at Downstate, 
      Brooklyn, NY 11203, USA.
FAU - Boruk, Marina
AU  - Boruk M
AD  - Department of Otolaryngology, State University of New York at Downstate, 
      Brooklyn, NY 11203, USA.
FAU - Suppa, Gabrielle
AU  - Suppa G
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY 11203, USA.
FAU - Foronjy, Robert
AU  - Foronjy R
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY 11203, USA.
FAU - Pato, Michele T
AU  - Pato MT
AD  - Department of Psychiatry, Rutgers University, Piscataway, NJ 08854, USA.
FAU - Pato, Carlos N
AU  - Pato CN
AD  - Department of Psychiatry, Rutgers University, Piscataway, NJ 08854, USA.
FAU - Knowles, James A
AU  - Knowles JA
AD  - Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, 
      USA.
FAU - Evgrafov, Oleg V
AU  - Evgrafov OV
AUID- ORCID: 0000-0003-0167-3939
AD  - Department of Cell Biology, State University of New York at Downstate, Brooklyn, 
      NY 11203, USA.
AD  - Human Genetics Institute of New Jersey, Rutgers University, Piscataway, NJ 08854, 
      USA.
LA  - eng
GR  - R01 MH086874/MH/NIMH NIH HHS/United States
GR  - Grant MH086874/MH/NIMH NIH HHS/United States
PT  - Journal Article
DEP - 20231019
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
UOF - bioRxiv. 2023 Mar 29;:. PMID: 37034711
MH  - Humans
MH  - Turbinates/pathology
MH  - *Schizophrenia/genetics/metabolism
MH  - Cells, Cultured
MH  - Neurons/metabolism
MH  - *Neural Stem Cells/metabolism
PMC - PMC10607243
OTO - NOTNLM
OT  - mesenchymal cells
OT  - middle turbinate
OT  - neurodevelopment
OT  - scRNA-seq
OT  - schizophrenia
COIS- The authors declare that their research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/10/28 11:48
MHDA- 2023/10/30 06:46
PMCR- 2023/10/19
CRDT- 2023/10/28 01:19
PHST- 2023/08/14 00:00 [received]
PHST- 2023/10/02 00:00 [revised]
PHST- 2023/10/10 00:00 [accepted]
PHST- 2023/10/30 06:46 [medline]
PHST- 2023/10/28 11:48 [pubmed]
PHST- 2023/10/28 01:19 [entrez]
PHST- 2023/10/19 00:00 [pmc-release]
AID - ijms242015339 [pii]
AID - ijms-24-15339 [pii]
AID - 10.3390/ijms242015339 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Oct 19;24(20):15339. doi: 10.3390/ijms242015339.