PMID- 3789524
OWN - NLM
STAT- MEDLINE
DCOM- 19870115
LR  - 20191105
IS  - 0003-0805 (Print)
IS  - 0003-0805 (Linking)
VI  - 134
IP  - 6
DP  - 1986 Dec
TI  - IgE antibody mediated inflammation of rat lung: histologic and bronchoalveolar 
      lavage assessment.
PG  - 1246-51
AB  - Pulmonary antigen challenge in sensitized individuals elicits immediate and late 
      phase responses (LPR). While mast cells and tissue inflammation are thought to be 
      vital to the development of the LPR, the precise pathogenesis of these responses 
      remains under investigation. Using the Sprague-Dawley rat as a model to study 
      cutaneous LPR, we have previously demonstrated that rat cutaneous LPR are mast 
      cell-dependent and are histologically characterized by early (1-8 h) 
      neutrophil-rich and late (8-24 h) mononuclear cell-rich infiltrates. To compare 
      and contrast this cutaneous response with IgE-dependent pulmonary inflammatory 
      responses, we performed bronchoalveolar lavage (BAL) analyses of pulmonary 
      inflammation following specific antigen challenge in actively immunized [IgE 
      anti-ovalbumin (OA) antibody] and BAL and histologic analyses in passively 
      sensitized [mouse hybridoma anti-dinitrophenyl (DNP) IgE antibody] rats. 
      Following direct insufflation of OA into the trachea, actively sensitized animals 
      demonstrated an increased number of polymorphonuclear leukocytes (PMNs) at 4 h in 
      BAL fluid. These cell numbers were significantly increased over controls by 24 h 
      following challenge. In addition, rats passively sensitized for 72 h with 
      anti-DNP IgE hybridoma antibody (PCA = 1:10,000) were challenged with DNP-BSA 
      aerosols. Examination of BAL fluid 1 to 2 h following challenge revealed 
      significantly increased numbers of PMNs which returned to normal levels by 24 h. 
      Numbers of lymphocytes and macrophages were unchanged compared to controls. 
      Microscopic examination of lung tissue revealed alveolar and interstitial edema 
      at 2 h following challenge and a focal peribronchiolitis characterized by a 
      predominantly mononuclear cell infiltrate.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Blythe, S
AU  - Blythe S
FAU - England, D
AU  - England D
FAU - Esser, B
AU  - Esser B
FAU - Junk, P
AU  - Junk P
FAU - Lemanske, R F Jr
AU  - Lemanske RF Jr
LA  - eng
GR  - 1 K08 HL01147-01/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am Rev Respir Dis
JT  - The American review of respiratory disease
JID - 0370523
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens/administration & dosage
MH  - Bronchi
MH  - Hypersensitivity, Delayed/immunology/pathology
MH  - Hypersensitivity, Immediate/immunology/pathology
MH  - Immunization/methods
MH  - Immunization, Passive/methods
MH  - Immunoglobulin E/*immunology
MH  - Lung/*immunology/pathology
MH  - Male
MH  - Mast Cells/immunology
MH  - Mice
MH  - Pneumonia/*etiology/immunology/pathology
MH  - Pulmonary Alveoli
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Therapeutic Irrigation
MH  - Time Factors
EDAT- 1986/12/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1986/12/01 00:00
PHST- 1986/12/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1986/12/01 00:00 [entrez]
AID - 10.1164/arrd.1986.134.5.1246 [doi]
PST - ppublish
SO  - Am Rev Respir Dis. 1986 Dec;134(6):1246-51. doi: 10.1164/arrd.1986.134.5.1246.