PMID- 37896867
OWN - NLM
STAT- MEDLINE
DCOM- 20231030
LR  - 20231103
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 15
IP  - 10
DP  - 2023 Oct 14
TI  - The Isolation and Characterization of a Novel Group III-Classified Getah Virus 
      from a Commercial Modified Live Vaccine against PRRSV.
LID - 10.3390/v15102090 [doi]
LID - 2090
AB  - As an epizootic causative agent, the Getah virus (GETV) can cause moderate 
      illness in horses, lethal disease in foxes, and reproductive disorders and fetal 
      death in pigs. Due to the wide range of hosts and multiple routes of 
      transmission, GETV has become a growing potential threat to the global livestock 
      industry, and even to public health. More attention and research on GETV are 
      urgently needed. In this study, we successfully isolated a novel GETV strain, 
      named BJ0304, from a commercial live vaccine against porcine reproductive and 
      respiratory syndrome virus (PRRSV) and determined its growth kinetics. Then, 
      genetic and phylogenetic analyses were performed. The results revealed that 
      BJ0304 was clustered into Group III, and it was most related to the GETV-V1 
      strain based on the complete genome sequence. Furthermore, the pathogenicity of 
      the isolate was assessed and found to be a low virulent strain in mice relative 
      to its closest homolog GETV-V1. Finally, mutation and glycosylation analysis 
      showed that a unique mutation (171 T > I) at one amino acid of E2, which affected 
      the glycosylation of E2, may be associated with viral pathogenicity. In summary, 
      the general characteristic of a novel Group III-classified GETV-BJ0304 isolated 
      from commercial live PRRSV vaccine was defined and then 
      mutation/glycosylation-related potential virulence factor was discussed. This 
      study highlights the complexity of GETV transmission routes in swine and the need 
      for more surveillance on commercial animal vaccines, contributes to the 
      understanding of genetic characterization of clinical isolates, provides possible 
      virulence factors in favor of unveiling the viral pathogenesis, and eventually 
      lays the foundation for the prevention and control of GETV.
FAU - Gao, Xintao
AU  - Gao X
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Li, Jialei
AU  - Li J
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Wu, Tong
AU  - Wu T
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Dou, Jinping
AU  - Dou J
AUID- ORCID: 0000-0001-9226-4676
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Zhang, Wenrong
AU  - Zhang W
AD  - College of Life Sciences, Capital Normal University, Beijing 100081, China.
FAU - Jia, Hong
AU  - Jia H
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100081, China.
FAU - Zhang, Zhifang
AU  - Zhang Z
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Liu, Xingjian
AU  - Liu X
AUID- ORCID: 0000-0001-8198-8089
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
FAU - Li, Yinu
AU  - Li Y
AUID- ORCID: 0000-0002-9310-8381
AD  - Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 
      Beijing 100081, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231014
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Vaccines)
SB  - IM
MH  - Animals
MH  - Swine
MH  - Horses
MH  - Mice
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Alphavirus/genetics
MH  - Phylogeny
MH  - *Vaccines
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
PMC - PMC10611409
OTO - NOTNLM
OT  - E2
OT  - Getah virus
OT  - PRRSV vaccine
OT  - mutation and glycosylation
OT  - pathogenicity
OT  - phylogenetic analysis
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/28 11:45
MHDA- 2023/10/30 06:47
PMCR- 2023/10/14
CRDT- 2023/10/28 01:29
PHST- 2023/09/21 00:00 [received]
PHST- 2023/10/11 00:00 [revised]
PHST- 2023/10/12 00:00 [accepted]
PHST- 2023/10/30 06:47 [medline]
PHST- 2023/10/28 11:45 [pubmed]
PHST- 2023/10/28 01:29 [entrez]
PHST- 2023/10/14 00:00 [pmc-release]
AID - v15102090 [pii]
AID - viruses-15-02090 [pii]
AID - 10.3390/v15102090 [doi]
PST - epublish
SO  - Viruses. 2023 Oct 14;15(10):2090. doi: 10.3390/v15102090.