PMID- 37897375
OWN - NLM
STAT- MEDLINE
DCOM- 20240209
LR  - 20240210
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 87
IP  - 3
DP  - 2024 Mar
TI  - Remote ischemic postconditioning alleviates cerebral ischemic injury through 
      SERCA2/endoplasmic reticulum stress-mediated apoptosis.
PG  - 424-433
LID - 10.1002/jemt.24431 [doi]
AB  - Remote ischemic postconditioning (RIPostC) alleviates brain ischemic injury 
      through several pathways, including endoplasmic reticulum (ER) stress modulation. 
      Sarco endoplasmic reticulum Ca(2+) -ATPase(SERCA2) which plays vital role in 
      calcium homeostasis regulation could modulate ER stress logically. This study 
      aimed to investigate whether RIPostC exerts its neuroprotective effect by 
      reducing ER stress mediated by SERCA2. Male SD rats underwent transient middle 
      cerebral artery occlusion (tMCAO) for 2 h followed by reperfusion, with the 
      RIPostC group undergoing 3 cycles of bilateral femoral artery clamping and 
      reperfusion at the beginning of reperfusion. Stroke outcome was assessed based on 
      infarct volume and neurological function evaluation. Protein levels of SERCA2 and 
      other ER stress markers were measured using Western blotting, immunofluorescence, 
      and immunohistochemistry techniques. Compared to the sham group, we observed that 
      RIPostC can effectively reduce cerebral infarct volume after I/R (34.55%: 21.03%; 
      p = .004) and improve neurological function deficit (9.67:12.5; p = .029). 
      Additionally, RIPostC increased SERCA2 protein expression and decreased the 
      protein level of glucose-regulated protein 78 (GRP78), phosphorylation of 
      eukaryotic translation initiation factor 2alpha (p-eIF2alpha) and CCAAT/EBP homologous 
      protein (CHOP). Furthermore, B-cell lymphoma-2 (Bcl-2) expression was increased, 
      while Bcl-2-associated X protein (Bax) and cleaved-caspase-3 was decreased in 
      response to application of RIPostC. Our results suggest that RIPostC improves the 
      prognosis of tMCAO rats, possibly by inhibiting the ER stress mediated by SERCA2, 
      facilitating apoptosis downregulation. The significance of this study is to 
      provide a theoretical basis for further exploring the protective mechanism of 
      ischemic stroke by RIPostC. RESEARCH HIGHLIGHTS: Our results suggest that RIPostC 
      improves the prognosis of tMCAO rats, possibly by inhibiting the ER stress 
      mediated by SERCA2, facilitating apoptosis downregulation, thus achieving a 
      neuroprotective effect.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Chen, Wanying
AU  - Chen W
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Li, Mengmeng
AU  - Li M
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Lei, Xiangyu
AU  - Lei X
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Cheng, Yawen
AU  - Cheng Y
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Wu, Xiaoyu
AU  - Wu X
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Sun, Xinyue
AU  - Sun X
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Wu, Yixuan
AU  - Wu Y
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Li, Zhiheng
AU  - Li Z
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Luo, Guogang
AU  - Luo G
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Wei, Meng
AU  - Wei M
AUID- ORCID: 0009-0004-5765-7414
AD  - Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
LA  - eng
GR  - 2019QN-13/First Affiliated Hospital of Xi'an Jiaotong University Foundation/
GR  - 2022JQ-987/Natural Science Foundation of Shaanxi Province/
GR  - XJTU1AF2021CRF-003/The Clinical Research Award of the First Affiliated Hospital 
      of Xi'an Jiaotong University, China/
GR  - XJTU1AF-CRF-2020-015/The Clinical Research Award of the First Affiliated Hospital 
      of Xi'an Jiaotong University, China/
PT  - Journal Article
DEP - 20231028
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Neuroprotective Agents)
SB  - IM
MH  - Rats
MH  - Male
MH  - Animals
MH  - *Ischemic Postconditioning
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - *Neuroprotective Agents
MH  - Apoptosis
MH  - Endoplasmic Reticulum Stress
OTO - NOTNLM
OT  - endoplasmic reticulum stress
OT  - remote ischemic postconditioning
OT  - sarco endoplasmic reticulum Ca2+-ATPase
OT  - transient middle cerebral artery occlusion
EDAT- 2023/10/29 06:42
MHDA- 2024/02/09 06:42
CRDT- 2023/10/28 09:03
PHST- 2023/09/18 00:00 [revised]
PHST- 2023/05/29 00:00 [received]
PHST- 2023/09/19 00:00 [accepted]
PHST- 2024/02/09 06:42 [medline]
PHST- 2023/10/29 06:42 [pubmed]
PHST- 2023/10/28 09:03 [entrez]
AID - 10.1002/jemt.24431 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2024 Mar;87(3):424-433. doi: 10.1002/jemt.24431. Epub 2023 Oct 
      28.