PMID- 37900941
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 1948-0210 (Print)
IS  - 1948-0210 (Electronic)
IS  - 1948-0210 (Linking)
VI  - 15
IP  - 9
DP  - 2023 Sep 26
TI  - Enhanced wound healing and hemostasis with exosome-loaded gelatin sponges from 
      human umbilical cord mesenchymal stem cells.
PG  - 947-959
LID - 10.4252/wjsc.v15.i9.947 [doi]
AB  - BACKGROUND: Rapid wound healing remains a pressing clinical challenge, 
      necessitating studies to hasten this process. A promising approach involves the 
      utilization of human umbilical cord mesenchymal stem cells (hUC-MSCs) derived 
      exosomes. The hypothesis of this study was that these exosomes, when loaded onto 
      a gelatin sponge, a common hemostatic material, would enhance hemostasis and 
      accelerate wound healing. AIM: To investigate the hemostatic and wound healing 
      efficacy of gelatin sponges loaded with hUC-MSCs-derived exosomes. METHODS: 
      Ultracentrifugation was used to extract exosomes from hUC-MSCs. Nanoparticle 
      tracking analysis (NTA), transmission electron microscopy (TEM), and western blot 
      techniques were used to validate the exosomes. In vitro experiments were 
      performed using L929 cells to evaluate the cytotoxicity of the exosomes and their 
      impact on cell growth and survival. New Zealand rabbits were used for skin 
      irritation experiments to assess whether they caused adverse skin reactions. 
      Hemolysis test was conducted using a 2% rabbit red blood cell suspension to 
      detect whether they caused hemolysis. Moreover, in vivo experiments were carried 
      out by implanting a gelatin sponge loaded with exosomes subcutaneously in 
      Sprague-Dawley (SD) rats to perform biocompatibility tests. In addition, 
      coagulation index test was conducted to evaluate their impact on blood 
      coagulation. Meanwhile, SD rat liver defect hemostasis model and full-thickness 
      skin defect model were used to study whether the gelatin sponge loaded with 
      exosomes effectively stopped bleeding and promoted wound healing. RESULTS: The 
      NTA, TEM, and western blot experimental results confirmed that exosomes were 
      successfully isolated from hUC-MSCs. The gelatin sponge loaded with exosomes did 
      not exhibit significant cell toxicity, skin irritation, or hemolysis, and they 
      demonstrated good compatibility in SD rats. Additionally, the effectiveness of 
      the gelatin sponge loaded with exosomes in hemostasis and wound healing was 
      validated. The results of the coagulation index experiment indicated that the 
      gelatin sponge loaded with exosomes had significantly better coagulation effect 
      compared to the regular gelatin sponge, and they showed excellent hemostatic 
      performance in a liver defect hemostasis model. Finally, the full-thickness skin 
      defect healing experiment results showed significant improvement in the healing 
      process of wounds treated with the gelatin sponge loaded with exosomes compared 
      to other groups. CONCLUSION: Collectively, the gelatin sponge loaded with 
      hUC-MSCs-derived exosomes is safe and efficacious for promoting hemostasis and 
      accelerating wound healing, warranting further clinical application.
CI  - (c)The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights 
      reserved.
FAU - Hu, Xin-Mei
AU  - Hu XM
AD  - Medical College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous 
      Region, China.
FAU - Wang, Can-Can
AU  - Wang CC
AD  - Medical College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous 
      Region, China.
FAU - Xiao, Yu
AU  - Xiao Y
AD  - Medical College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous 
      Region, China.
FAU - Jiang, Peng
AU  - Jiang P
AD  - Medical College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous 
      Region, China.
FAU - Liu, Yu
AU  - Liu Y
AD  - Medical College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous 
      Region, China.
FAU - Qi, Zhong-Quan
AU  - Qi ZQ
AD  - Medical College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous 
      Region, China. yxyyz@gxu.edu.cn.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Stem Cells
JT  - World journal of stem cells
JID - 101535826
PMC - PMC10600743
OTO - NOTNLM
OT  - Exosomes
OT  - Gelatin sponge
OT  - Hemostasis
OT  - Human umbilical cord mesenchymal stem cells
OT  - Safety
OT  - Wound healing
COIS- Conflict-of-interest statement: All the authors report no relevant conflicts of 
      interest for this article.
EDAT- 2023/10/30 06:47
MHDA- 2023/10/30 06:48
PMCR- 2023/09/26
CRDT- 2023/10/30 04:42
PHST- 2023/08/14 00:00 [received]
PHST- 2023/08/31 00:00 [revised]
PHST- 2023/09/14 00:00 [accepted]
PHST- 2023/10/30 06:48 [medline]
PHST- 2023/10/30 06:47 [pubmed]
PHST- 2023/10/30 04:42 [entrez]
PHST- 2023/09/26 00:00 [pmc-release]
AID - 10.4252/wjsc.v15.i9.947 [doi]
PST - ppublish
SO  - World J Stem Cells. 2023 Sep 26;15(9):947-959. doi: 10.4252/wjsc.v15.i9.947.