PMID- 37902912
OWN - NLM
STAT- Publisher
LR  - 20231030
IS  - 1573-4927 (Electronic)
IS  - 0006-2928 (Linking)
DP  - 2023 Oct 30
TI  - Integrated Network Pharmacology and Molecular Docking to Elucidate the Efficacy 
      and Potential Mechanisms of Tea Ingredients in Sepsis Treatment.
LID - 10.1007/s10528-023-10530-6 [doi]
AB  - Sepsis, a critical health condition induced by an overactive innate immune 
      response and reactive oxygen species (ROS)-driven host damage through apoptosis 
      and ferroptosis, continues to pose a significant mortality risk. Despite 
      accumulating evidence of the potential therapeutic properties of tea ingredients, 
      their specific anti-sepsis potential remains inadequately explored. This study 
      comprehensively investigates the targeted genes of tea ingredients, notably 
      epigallocatechin 3-gallate (EGCG), and their correlation with sepsis signature 
      genes. Our findings elucidate that tea ingredients, especially EGCG, exhibit 
      substantial potential in mitigating inflammation and sepsis-induced damage. 
      Through the inhibition of the MAPK cascade and macrophage activation and by 
      impeding the transcriptional activity of RELA (transcription factor p65) in 
      sepsis, EGCG demonstrates significant anti-sepsis efficacy. Molecular docking 
      analysis further underpins this by revealing the close proximity of EGCG and 
      (-)-catechin gallate binding sites to that of RELA on DNA. Subsequent in vitro 
      assays illuminated EGCG's instrumental role in modulating macrophage M2 
      polarization, balancing M1 and M2 differentiation of bone marrow-derived 
      macrophages (BMDMs), curtailing inflammatory factor secretion, and inhibiting ROS 
      production. Moreover, EGCG effectively suppresses the expression of 
      ferroptosis/apoptosis markers in LPS-induced macrophages during their early 
      stages. Our study advances our understanding of sepsis prevention and treatment 
      strategies, suggesting that tea ingredients such as EGCG could play a pivotal 
      role in developing future sepsis therapies due to their protective effects.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional 
      Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 
      210028, China.
AD  - Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China.
FAU - Jiang, Ye
AU  - Jiang Y
AD  - Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional 
      Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 
      210028, China.
AD  - Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China.
FAU - Tao, Qing
AU  - Tao Q
AD  - Center for Translational Medicine and Jiangsu Key Laboratory of Molecular 
      Medicine, Medical School, Nanjing University, Nanjing, 210093, Jiangsu, China.
FAU - Shi, Jianfeng
AU  - Shi J
AD  - Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional 
      Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 
      210028, China.
AD  - Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China.
FAU - Lu, Min
AU  - Lu M
AD  - Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China. 
      lumin_njucm@163.com.
AD  - Gastroenterology Department, Affiliated Hospital of Integrated Traditional 
      Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 
      210028, China. lumin_njucm@163.com.
FAU - Yao, Xiaoming
AU  - Yao X
AD  - Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional 
      Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 
      210028, China. yaoxm73@sina.com.
AD  - Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China. 
      yaoxm73@sina.com.
LA  - eng
GR  - 82202364/National Natural Science Foundation of China/
GR  - 2020YFC2005300/National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20231030
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
SB  - IM
OTO - NOTNLM
OT  - EGCG
OT  - Molecular docking
OT  - Network pharmacology
OT  - Sepsis
OT  - Tea ingredients
EDAT- 2023/10/30 18:43
MHDA- 2023/10/30 18:43
CRDT- 2023/10/30 12:07
PHST- 2023/06/23 00:00 [received]
PHST- 2023/09/15 00:00 [accepted]
PHST- 2023/10/30 18:43 [medline]
PHST- 2023/10/30 18:43 [pubmed]
PHST- 2023/10/30 12:07 [entrez]
AID - 10.1007/s10528-023-10530-6 [pii]
AID - 10.1007/s10528-023-10530-6 [doi]
PST - aheadofprint
SO  - Biochem Genet. 2023 Oct 30. doi: 10.1007/s10528-023-10530-6.