PMID- 37904947
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
DP  - 2023 Oct 17
TI  - Rapid and high yield isolation of extracellular vesicles with purity by 
      application of size exclusion fast performance liquid chromatography.
LID - 2023.10.12.561425 [pii]
LID - 10.1101/2023.10.12.561425 [doi]
AB  - Extracellular Vesicles (EVs) have emerged as potential biomarkers for diagnosing 
      a range of diseases without invasive procedures. Extracellular vesicles also 
      offer an advantage compared to synthetic vesicles, for delivery of various drugs. 
      However, limitations in segregating EVs from soluble proteins have led to 
      inconsistent EV retrieval rates with low levels of purity. Here, we report a new 
      high-yield (>95%) and rapid (<20 min) EV isolation method called S ize E xclusion 
      - F ast P erformance L iquid C hromatography (SE-FPLC). We show SE-FPLC can 
      effectively isolate EVs from multiple sources including EVs derived from human 
      and mouse cells and serum. The results indicate that SE-FPLC can successfully 
      remove highly abundant protein contaminants such as albumin and lipoprotein 
      complexes, which can represent a major hurdle in large scale isolation of EVs for 
      clinical translation. Additionally, the high-yield nature of SE- FPLC allows for 
      easy industrial upscaling of extracellular vesicles production for various 
      clinical utilities. Moreover, SE-FPLC enables analysis of very small volumes of 
      blood for use in point-of-care diagnostics in the clinic. Collectively, SE-FPLC 
      offers many advantages over current EV isolation methods and offers rapid 
      clinical utility potential.
FAU - Kapoor, Kshipra S
AU  - Kapoor KS
FAU - Harris, Kristen
AU  - Harris K
FAU - Arian, Kent
AU  - Arian K
FAU - Ma, Lihua
AU  - Ma L
FAU - Church, Kaira A
AU  - Church KA
FAU - Kalluri, Raghu
AU  - Kalluri R
LA  - eng
PT  - Preprint
DEP - 20231017
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
PMC - PMC10614745
EDAT- 2023/10/31 06:42
MHDA- 2023/10/31 06:43
PMCR- 2023/10/30
CRDT- 2023/10/31 03:59
PHST- 2023/10/31 06:43 [medline]
PHST- 2023/10/31 06:42 [pubmed]
PHST- 2023/10/31 03:59 [entrez]
PHST- 2023/10/30 00:00 [pmc-release]
AID - 2023.10.12.561425 [pii]
AID - 10.1101/2023.10.12.561425 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Oct 17:2023.10.12.561425. doi: 
      10.1101/2023.10.12.561425.