PMID- 37914354
OWN - NLM
STAT- MEDLINE
DCOM- 20231103
LR  - 20231103
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 46
IP  - 11
DP  - 2023
TI  - RasGRP2 Attenuates Oxygen Deprivation-Induced Autophagy in Vascular Endothelial 
      Cells.
PG  - 1512-1516
LID - 10.1248/bpb.b23-00317 [doi]
AB  - Vascular endothelial cells sustain vascular health through barrier and endocrine 
      functions. Insufficient oxygen supply induces endothelial dysfunction in the 
      pathology of various diseases. In addition, oxygen deprivation reportedly induces 
      endothelial dysfunction via autophagy. Ras guanyl-releasing protein 2 (RasGRP2) 
      has guanosine 5'-diphosphate (GDP)/guanosine 5'-triphosphate (GTP) exchange 
      factor activity and activates Rap1 and R-Ras which belong to the small GTPases. 
      RasGRP2 exerts protective effects against vascular endothelial dysfunction. 
      However, the effect of RasGRP2 on hypoxic stress in vascular endothelial cells 
      has not yet been investigated. We examined the protein expression of 
      hypoxia-inducible factor (HIF)-1alpha, BCL2 interacting protein 3 (BNIP3), and 
      microtubule-associated protein light chain 3beta (LC3beta). We observed that oxygen 
      deprivation increased the expression of HIF-1alpha, BNIP3 and LC3beta II. RasGRP2 
      suppressed the induction of HIF-1alpha and the subsequent increase in LC3beta II. These 
      findings suggest the possibility that RasGRP2 plays a protective role against 
      endothelial dysfunction by suppressing oxygen deprivation-induced autophagy.
FAU - Miyazaki, Shouhei
AU  - Miyazaki S
AD  - Faculty of Pharmaceutical Sciences, Hiroshima International University.
FAU - Takino, Jun-Ichi
AU  - Takino JI
AD  - Faculty of Pharmaceutical Sciences, Hiroshima International University.
FAU - Nagamine, Kentaro
AU  - Nagamine K
AD  - Faculty of Health Sciences, Hiroshima International University.
FAU - Hori, Takamitsu
AU  - Hori T
AD  - Faculty of Pharmaceutical Sciences, Hiroshima International University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - S88TT14065 (Oxygen)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
SB  - IM
MH  - *Endothelial Cells/metabolism
MH  - *Oxygen/metabolism
MH  - Guanine Nucleotide Exchange Factors
MH  - Microtubule-Associated Proteins/metabolism
MH  - Autophagy
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Cell Hypoxia
OTO - NOTNLM
OT  - Ras guanyl-releasing protein 2
OT  - autophagy
OT  - endothelial cell
OT  - hypoxia
OT  - hypoxia-inducible factor-1alpha
EDAT- 2023/11/02 00:42
MHDA- 2023/11/03 06:43
CRDT- 2023/11/01 21:05
PHST- 2023/11/03 06:43 [medline]
PHST- 2023/11/02 00:42 [pubmed]
PHST- 2023/11/01 21:05 [entrez]
AID - 10.1248/bpb.b23-00317 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2023;46(11):1512-1516. doi: 10.1248/bpb.b23-00317.