PMID- 37920136
OWN - NLM
STAT- MEDLINE
DCOM- 20240219
LR  - 20240219
IS  - 1755-148X (Electronic)
IS  - 1755-1471 (Linking)
VI  - 37
IP  - 2
DP  - 2024 Mar
TI  - Cytoglobin functions as a redox regulator of melanogenesis in normal epidermal 
      melanocytes.
PG  - 276-285
LID - 10.1111/pcmr.13146 [doi]
AB  - Epidermal melanocytes are continuously exposed to sunlight-induced reactive 
      oxygen species (ROS) and oxidative stress generated during the synthesis of 
      melanin. Therefore, they have developed mechanisms that maintain normal redox 
      homeostasis. Cytoglobin (CYGB), a ubiquitously expressed intracellular iron 
      hexacoordinated globin, exhibits antioxidant activity and regulates the redox 
      state of mammalian cells through its activities as peroxidase and nitric oxide 
      (NO) dioxygenase. We postulated that CYGB functions in the melanogenic process as 
      a regulator that maintains oxidative stress within a physiological level. This 
      was examined by characterizing normal human melanocytes with the knockdown (KD) 
      of CYGB using morphological and molecular biological criteria. CYGB-KD cells were 
      larger, had more dendrites, and generated more melanin granules in the advanced 
      stages of melanogenesis than control cells. The expression levels of major 
      melanogenesis-associated genes and proteins were higher in CYGB-KD melanocytes 
      than in wild type (WT) cells. As expected, CYGB-KD melanocytes generated more ROS 
      and NO than WT cells. In conclusion, CYGB physiologically contributes to 
      maintaining redox homeostasis in the melanogenic activity of normal melanocytes 
      by controlling the intracellular levels of ROS and NO.
CI  - (c) 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Tanaka, Yo
AU  - Tanaka Y
AUID- ORCID: 0009-0008-0652-7096
AD  - Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
FAU - Sato-Matsubara, Misako
AU  - Sato-Matsubara M
AUID- ORCID: 0000-0003-0593-7305
AD  - Department of Hepatology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka, Japan.
AD  - Donated Synthetic Biology Laboratory, Osaka Metropolitan University Graduate 
      School of Medicine, Osaka, Japan.
FAU - Tsuruta, Daisuke
AU  - Tsuruta D
AUID- ORCID: 0000-0001-6283-0251
AD  - Department of Dermatology, Osaka Metropolitan University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Tanaka, Hiroshi
AU  - Tanaka H
AUID- ORCID: 0009-0009-6631-5949
AD  - Research Laboratories, Nippon Menard Cosmetic Co., Ltd., Nagoya, Japan.
FAU - Kadono, Chiho
AU  - Kadono C
AUID- ORCID: 0000-0002-0686-7305
AD  - Donated Synthetic Biology Laboratory, Osaka Metropolitan University Graduate 
      School of Medicine, Osaka, Japan.
FAU - Sugawara, Koji
AU  - Sugawara K
AUID- ORCID: 0000-0003-1662-3272
AD  - Department of Dermatology, Osaka Metropolitan University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Kawada, Norifumi
AU  - Kawada N
AUID- ORCID: 0000-0002-6392-8311
AD  - Department of Hepatology, Graduate School of Medicine, Osaka Metropolitan 
      University, Osaka, Japan.
FAU - Wakamatsu, Kazumasa
AU  - Wakamatsu K
AUID- ORCID: 0000-0003-1748-9001
AD  - Institute for Melanin Chemistry, Fujita Health University, Toyoake, Japan.
FAU - Ito, Shosuke
AU  - Ito S
AUID- ORCID: 0000-0001-9182-5144
AD  - Institute for Melanin Chemistry, Fujita Health University, Toyoake, Japan.
FAU - Yoshizato, Katsutoshi
AU  - Yoshizato K
AUID- ORCID: 0000-0003-2133-7983
AD  - Donated Synthetic Biology Laboratory, Osaka Metropolitan University Graduate 
      School of Medicine, Osaka, Japan.
LA  - eng
PT  - Journal Article
DEP - 20231102
PL  - England
TA  - Pigment Cell Melanoma Res
JT  - Pigment cell & melanoma research
JID - 101318927
RN  - 0 (Cytoglobin)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Melanins)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Cytoglobin/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - *Melanins/metabolism
MH  - *Melanogenesis
MH  - Melanocytes/metabolism
MH  - Oxidation-Reduction
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - cytoglobin
OT  - epidermal melanocytes
OT  - melanogenesis
OT  - nitric oxide
OT  - reactive oxygen species
EDAT- 2023/11/03 06:44
MHDA- 2024/02/19 06:43
CRDT- 2023/11/03 03:46
PHST- 2023/09/21 00:00 [revised]
PHST- 2023/05/15 00:00 [received]
PHST- 2023/10/15 00:00 [accepted]
PHST- 2024/02/19 06:43 [medline]
PHST- 2023/11/03 06:44 [pubmed]
PHST- 2023/11/03 03:46 [entrez]
AID - 10.1111/pcmr.13146 [doi]
PST - ppublish
SO  - Pigment Cell Melanoma Res. 2024 Mar;37(2):276-285. doi: 10.1111/pcmr.13146. Epub 
      2023 Nov 2.