PMID- 37920678
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231104
IS  - 1654-661X (Print)
IS  - 1654-661X (Electronic)
IS  - 1654-661X (Linking)
VI  - 67
DP  - 2023
TI  - A synergistic blend of Garcinia mangostana fruit rind and Cinnamomum tamala leaf 
      extracts enhances myogenic differentiation and mitochondrial biogenesis in vitro 
      and muscle growth and strength in mice.
LID - 10.29219/fnr.v67.9750 [doi]
AB  - BACKGROUND: A proprietary combination of Garcinia mangostana fruit rind and 
      Cinnamomum tamala leaf extracts (LI80020F4, CinDura((R))) improved the physical 
      performance and muscle strength of resistance-trained adult males. OBJECTIVE: 
      This study assessed the underlying mechanisms of the ergogenic potential of 
      LI80020F4 in in vitro and in vivo models. METHODS: The individual extracts and 
      their combination (LI80020F4) were assessed for nitrite production in EAhy926 
      human endothelial cells. Subsequent experiments evaluated the effect of LI80020F4 
      in myotube formation in C2C12 mouse myoblasts, expression of mammalian target of 
      rapamycin (mTOR) signaling proteins, myogenic factors, and mitochondrial 
      functions in L6 rat myoblasts.Moreover, adult male ICR mice were randomly 
      assigned (n = 15) into vehicle control (G1), exercise alone (G2), oxymetholone-16 
      mg/kg body weight (bw) (G3), and 75 (G4)-, 150 (G5)-, or 300 (G6) mg/kg bw of 
      LI80020F4, orally gavaged for 28 days. G1 and G2 mice received 0.5% 
      carboxymethylcellulose sodium. Following completion, muscle strength and physical 
      performance were assessed on forelimb grip strength and forced swimming test 
      (FST), respectively. Gastrocnemius (GA), tibialis anterior (TA) muscle weights, 
      muscle fiber cross-sectional area (CSA), levels of muscle, and serum protein 
      markers were also determined. RESULTS: LI80020F4 increased nitrite production in 
      EAhy926 cells in a dose-dependent manner. LI80020F4 induced C2C12 myotube 
      formation, increased mitochondrial biogenesis, upregulated the expressions of 
      activated mTOR and other mitochondria and myogenic proteins, and mitigated 
      H(2)O(2)-induced mitochondrial membrane depolarization in the myoblast cells. In 
      the animal study, 75, 150, and 300 mg/kg bw LI80020F4 doses significantly (P < 
      0.05) increased the animals' forelimb grip strength. Mid- and high-dose groups 
      showed increased swimming time, increased muscle weight, CSA, muscle 
      growth-related, and mitochondrial protein expressions in the GA muscles. 
      CONCLUSION: LI80020F4 increases nitric oxide production in the endothelial cells, 
      mitochondrial biogenesis and function, upregulates skeletal muscle growth-related 
      protein expressions and reduces oxidative stress; together, it explains the basis 
      of the ergogenic potential of LI80020F4.
CI  - (c) 2023 Swaraj Sinha et al.
FAU - Sinha, Swaraj
AU  - Sinha S
AD  - Department of Cell and Molecular Biology, Laila Nutraceuticals R&D Center, 
      Vijayawada, Andhra Pradesh, India.
FAU - Alluri, Krishnaraju Venkata
AU  - Alluri KV
AD  - Department of Pharmacology and Clinical Research, Laila Nutraceuticals R&D 
      Center, Vijayawada, Andhra Pradesh, India.
FAU - Somepalli, Venkateswarlu
AU  - Somepalli V
AD  - Department of Phytochemistry, Laila Nutraceuticals R&D Center, Vijayawada, Andhra 
      Pradesh, India.
FAU - Golakoti, Trimurtulu
AU  - Golakoti T
AD  - Department of Phytochemistry, Laila Nutraceuticals R&D Center, Vijayawada, Andhra 
      Pradesh, India.
FAU - Sengupta, Krishanu
AU  - Sengupta K
AD  - Department of Cell and Molecular Biology, Laila Nutraceuticals R&D Center, 
      Vijayawada, Andhra Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20231030
PL  - Sweden
TA  - Food Nutr Res
JT  - Food & nutrition research
JID - 101488795
PMC - PMC10619412
OTO - NOTNLM
OT  - broad-spectrum safety
OT  - ergogenic phytonutrients
OT  - mammalian target of rapamycin (mTOR)
OT  - mitochondrial biogenesis
OT  - muscle growth and strength
OT  - nitric oxide
COIS- Authors declare that they have conflicts of interest. Laila nutraceuticals, 
      Vijayawada, Andhra Pradesh, India funded the study.
EDAT- 2023/11/03 06:44
MHDA- 2023/11/03 06:45
PMCR- 2023/10/30
CRDT- 2023/11/03 04:01
PHST- 2023/06/05 00:00 [received]
PHST- 2023/07/11 00:00 [revised]
PHST- 2023/07/19 00:00 [accepted]
PHST- 2023/11/03 06:45 [medline]
PHST- 2023/11/03 06:44 [pubmed]
PHST- 2023/11/03 04:01 [entrez]
PHST- 2023/10/30 00:00 [pmc-release]
AID - 9750 [pii]
AID - 10.29219/fnr.v67.9750 [doi]
PST - epublish
SO  - Food Nutr Res. 2023 Oct 30;67. doi: 10.29219/fnr.v67.9750. eCollection 2023.