PMID- 37924676
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20231201
IS  - 1878-4291 (Electronic)
IS  - 0968-4328 (Linking)
VI  - 176
DP  - 2024 Jan
TI  - Cellular evidence of communication strategies for small intestinal high 
      endothelial cells: Ultrastructural insights of nano-scale exosomes and autophagy.
PG  - 103559
LID - S0968-4328(23)00157-9 [pii]
LID - 10.1016/j.micron.2023.103559 [doi]
AB  - Although several immune related cells of small intestine play an essential role 
      in the intestinal homeostasis. However, information related to ultrastructural 
      evidence of Nano-scale exosomes-multivesicular bodies and autophagic pathway in 
      the high endothelial cells (HECs) of the small intestine in laying birds is still 
      ambiguous. In present study, the HECs secreted the early endosome (ee), late 
      endosome (le) and multivesicular bodies (MVBs) in the lamina propria of layer 
      small intestine was confirmed by transmission electron microscopy. Besides that, 
      in the cytoplasm of HECs showed many autophagosomes were directly associated with 
      lysosomes and mitochondria. Further, the immunohistochemistry and 
      immunofluorescence results showed that, the immunoreactivity and immuno-signaling 
      of Nano-scale exosome related proteins, cluster of differentiation (CD63) and 
      tumor susceptibility gene (TSG101), and autophagic related proteins, autophagic 
      related gene (ATG7) and microtubule-associated protein light chain (LC3) were 
      strong positive expression in the lamina propria of small intestine. These 
      results prove that HECs play a well-known immunological role in the maintenance 
      of intestinal homeostasis. In summary, these findings indicate that the small 
      intestine's HECs have developed an innovative way of communication.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Hao, Ping
AU  - Hao P
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Yin, Wen
AU  - Yin W
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Chen, Xi
AU  - Chen X
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Qin, Shuangshuang
AU  - Qin S
AD  - Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/ 
      Guangxi Engineering Research Center of TCM Resource Intelligent Creation, 
      National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of 
      Medicinal Plants, Nanning 530023, PR China.
FAU - Yu, Yue
AU  - Yu Y
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Yuan, Yuan
AU  - Yuan Y
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Quan, Xiaoyu
AU  - Quan X
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Hu, Bing
AU  - Hu B
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Chen, Shouhai
AU  - Chen S
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China.
FAU - Wu, Yi
AU  - Wu Y
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095 Jiangsu 
      Province, PR China. Electronic address: wuyi2001cn@163.com.
LA  - eng
PT  - Journal Article
DEP - 20231018
PL  - England
TA  - Micron
JT  - Micron (Oxford, England : 1993)
JID - 9312850
SB  - IM
MH  - *Exosomes/ultrastructure
MH  - Endothelial Cells
MH  - Autophagy
MH  - Multivesicular Bodies/chemistry/metabolism/ultrastructure
MH  - Intestine, Small
OTO - NOTNLM
OT  - Autophagy
OT  - Exosomes
OT  - High endothelial cell
OT  - Multivesicular bodies
OT  - Small intestine
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2023/11/05 00:42
MHDA- 2023/11/27 12:45
CRDT- 2023/11/04 19:07
PHST- 2023/09/16 00:00 [received]
PHST- 2023/10/14 00:00 [revised]
PHST- 2023/10/17 00:00 [accepted]
PHST- 2023/11/27 12:45 [medline]
PHST- 2023/11/05 00:42 [pubmed]
PHST- 2023/11/04 19:07 [entrez]
AID - S0968-4328(23)00157-9 [pii]
AID - 10.1016/j.micron.2023.103559 [doi]
PST - ppublish
SO  - Micron. 2024 Jan;176:103559. doi: 10.1016/j.micron.2023.103559. Epub 2023 Oct 18.