PMID- 37925634
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 4
IP  - 4
DP  - 2023 Dec 15
TI  - Protocol for the expansion of mouse immune effector cells for in vitro and 
      in vivo studies.
PG  - 102700
LID - S2666-1667(23)00667-6 [pii]
LID - 10.1016/j.xpro.2023.102700 [doi]
LID - 102700
AB  - Reproducible and efficient expansion of different immune effector cells is 
      required for pre-clinical studies investigating adoptive cell therapies against 
      cancer. Here, we provide a protocol for the rapid expansion of mouse T cells, 
      natural killer (NK) cells, and bone-marrow-derived macrophages (BMDMs). We 
      describe steps for alphaCD3/alphaCD8 plate coating, isolating splenocytes, and expanding 
      T cells and NK cells. Further, we detail procedures for bone marrow isolation and 
      BMDM differentiation.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Look, Thomas
AU  - Look T
AD  - Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 
      Zurich, Switzerland. Electronic address: thomas.look@usz.ch.
FAU - Meister, Hanna
AU  - Meister H
AD  - Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 
      Zurich, Switzerland.
FAU - Weller, Michael
AU  - Weller M
AD  - Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 
      Zurich, Switzerland; University Hospital Zurich, 8091 Zurich, Switzerland.
FAU - Weiss, Tobias
AU  - Weiss T
AD  - Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 
      Zurich, Switzerland; University Hospital Zurich, 8091 Zurich, Switzerland. 
      Electronic address: tobias.weiss@usz.ch.
LA  - eng
PT  - Journal Article
DEP - 20231109
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Killer Cells, Natural
MH  - *Neoplasms/therapy
MH  - T-Lymphocytes
MH  - Bone Marrow
PMC - PMC10751566
OTO - NOTNLM
OT  - Cancer
OT  - Cell Biology
OT  - Cell culture
OT  - Cell isolation
OT  - Flow Cytometry
OT  - Immunology
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2023/11/05 18:42
MHDA- 2024/03/11 06:43
PMCR- 2023/11/09
CRDT- 2023/11/05 12:36
PHST- 2023/09/19 00:00 [received]
PHST- 2023/10/09 00:00 [revised]
PHST- 2023/10/17 00:00 [accepted]
PHST- 2024/03/11 06:43 [medline]
PHST- 2023/11/05 18:42 [pubmed]
PHST- 2023/11/05 12:36 [entrez]
PHST- 2023/11/09 00:00 [pmc-release]
AID - S2666-1667(23)00667-6 [pii]
AID - 102700 [pii]
AID - 10.1016/j.xpro.2023.102700 [doi]
PST - ppublish
SO  - STAR Protoc. 2023 Dec 15;4(4):102700. doi: 10.1016/j.xpro.2023.102700. Epub 2023 
      Nov 9.