PMID- 37925811
OWN - NLM
STAT- MEDLINE
DCOM- 20240129
LR  - 20240206
IS  - 1808-8686 (Electronic)
IS  - 1808-8694 (Print)
IS  - 1808-8686 (Linking)
VI  - 90
IP  - 1
DP  - 2024 Jan-Feb
TI  - Nasopharyngeal carcinoma derived exosomes regulate the proliferation and 
      migration of nasopharyngeal carcinoma cells by mediating the miR-99a-5p BAZ2A 
      axis.
PG  - 101343
LID - S1808-8694(23)00111-8 [pii]
LID - 10.1016/j.bjorl.2023.101343 [doi]
LID - 101343
AB  - OBJECTIVES: Nasopharyngeal Carcinoma (NPC) is a common malignant tumor of 
      nasopharyngeal mucosal epithelium in clinical practice. Radiotherapy and 
      chemotherapy are the main treatment methods at present, but the therapeutic 
      effect is still unsatisfactory. Studies have shown that exosomes and microRNAs 
      (miRNAs) play an important role in the development of cancer. Therefore, this 
      study aimed to investigate the effects of NPC derived exosomes on NPC and their 
      molecular mechanisms. METHODS: Serum was collected from healthy subjects, 
      Epstein-Barr Virus (EBV) infected patients and NPC patients (n = 9 group) and 
      exosomes were extracted separately. High-throughput sequencing of exosomes was 
      performed to screen differentially expressed miRNAs. The function of the screened 
      miRNA was identified by treating NPC cells with exosomes. The target gene of 
      miRNA was identified using the dual-luciferase assay. Real-Time quantitative 
      Polymerase Chain Reaction (RT-qPCR) was used to determine the levels of 
      miR-99a-5p and Bromodomain Adjacent Tozinc finger domain protein 2A (BAZ2A). Cell 
      Counting Kit-8 assay, flow cytometry, and wound healing assay were utilized to 
      detect cell viability, cell cycle and apoptosis, and migration ability. The 
      protein levels were evaluated by Western blot. RESULTS: MiR-99a-5p was identified 
      as the most significant differentially expressed miRNA in exosomes (p < 0.05). 
      The proliferation and migration of NPC cells were extremely facilitated by 
      exosomes, accompanied by the suppressed apoptosis, upregulated BAZ2A, Monocyte 
      Chemotactic Protein-1 (MCP1), and Vascular Endothelial Growth Factor A (VEGFA), 
      and downregulation of Interleukin (IL)-1beta and Nuclear Transcription Factor-kappaB 
      (NF-kappaB) (p < 0.05). BAZ2A was a target gene of miR-99a-5p. Furthermore, the 
      regulatory effect of exosomes on the proliferation, migration, and apoptosis was 
      significantly abolished by overexpression of miR-99a-5p or downregulation of 
      BAZ2A (p < 0.05). CONCLUSION: NPC derived exosomes facilitated the proliferation 
      and migration of NPC through regulating the miR-99a-5p/BAZ2A axis.
CI  - Copyright (c) 2023 Associacao Brasileira de Otorrinolaringologia e Cirurgia 
      Cervico-Facial. Published by Elsevier Espana S.L.U. All rights reserved.
FAU - Zhou, Xiao-Jun
AU  - Zhou XJ
AD  - Integrated Hospital of Traditional Chinese Medicine of Southern Medical 
      University, Department of Otolaryngology, Guangzhou, China. Electronic address: 
      zd1232@126.com.
FAU - Xu, Hang-Min
AU  - Xu HM
AD  - Zhongshan Traditional Chinese Medicine Hospital Affiliated to Guangzhou 
      University of Chinese Medicine, Zhongshan, China.
FAU - Huang, Guo-Sen
AU  - Huang GS
AD  - Zhongshan Traditional Chinese Medicine Hospital Affiliated to Guangzhou 
      University of Chinese Medicine, Zhongshan, China.
FAU - Lin, Bao-Rui
AU  - Lin BR
AD  - Zhongshan Traditional Chinese Medicine Hospital Affiliated to Guangzhou 
      University of Chinese Medicine, Zhongshan, China.
LA  - eng
PT  - Journal Article
DEP - 20231011
PL  - Brazil
TA  - Braz J Otorhinolaryngol
JT  - Brazilian journal of otorhinolaryngology
JID - 101207337
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (MicroRNAs)
RN  - 0 (BAZ2A protein, human)
RN  - 0 (Bromodomain Containing Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
SB  - IM
MH  - Humans
MH  - Nasopharyngeal Carcinoma/pathology
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
MH  - *Exosomes/genetics/metabolism/pathology
MH  - *Epstein-Barr Virus Infections/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Herpesvirus 4, Human/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Nasopharyngeal Neoplasms/genetics/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - Bromodomain Containing Proteins
MH  - Chromosomal Proteins, Non-Histone/genetics/metabolism
PMC - PMC10654546
OTO - NOTNLM
OT  - BAZ2A
OT  - Exosomes
OT  - MiR-99a-5p
OT  - Migration
OT  - Nasopharyngeal carcinoma
OT  - Proliferation
EDAT- 2023/11/06 00:41
MHDA- 2024/01/29 06:42
PMCR- 2023/10/11
CRDT- 2023/11/05 18:05
PHST- 2023/07/09 00:00 [received]
PHST- 2023/09/17 00:00 [revised]
PHST- 2023/10/02 00:00 [accepted]
PHST- 2024/01/29 06:42 [medline]
PHST- 2023/11/06 00:41 [pubmed]
PHST- 2023/11/05 18:05 [entrez]
PHST- 2023/10/11 00:00 [pmc-release]
AID - S1808-8694(23)00111-8 [pii]
AID - 101343 [pii]
AID - 10.1016/j.bjorl.2023.101343 [doi]
PST - ppublish
SO  - Braz J Otorhinolaryngol. 2024 Jan-Feb;90(1):101343. doi: 
      10.1016/j.bjorl.2023.101343. Epub 2023 Oct 11.