PMID- 37925864
OWN - NLM
STAT- MEDLINE
DCOM- 20231205
LR  - 20231228
IS  - 1872-7603 (Electronic)
IS  - 0165-0378 (Linking)
VI  - 160
DP  - 2023 Dec
TI  - Sleep deprivation during pregnancy leads to poor fetal outcomes in Sprague-Dawley 
      rats.
PG  - 104166
LID - S0165-0378(23)00372-8 [pii]
LID - 10.1016/j.jri.2023.104166 [doi]
AB  - Sleep deprivation is a common problem during pregnancy, but its impact on the 
      fetus remains unclear. We aimed to investigate the effect of sleep deprivation 
      during pregnancy on fetal outcomes and its mechanism in Sprague-Dawley rats. 
      Sleep deprivation was performed from gestational day(GD) 1-19 using a 
      multiplatform method for 18 h/day. Rats were sacrificed on GD20, and their blood 
      and placentas were collected. Fetal and placental parameters were ascertained. 
      Melatonin, adrenocorticotropic hormone (ACTH) and corticosterone were also 
      measured in serum. The levels of arylalkylamine N-acetyltransferase (AANAT) and 
      two melatonin receptors MT1 and MT2, in placental tissues were detected by 
      western blotting. The inflammatory status and oxidative stress in serum and 
      placentas were investigated. Miscarriage and intrauterine growth restriction were 
      observed in the sleep deprivation group. Sleep deprivation resulted in an 
      increased fetal absorption rate, while fetal weight, crown-rump length and 
      placental weight were reduced. Placental histopathology showed that the labyrinth 
      ratio in the sleep deprivation group was significantly reduced, with hypoplastic 
      villi and obviously decreased blood vessels. Sleep deprivation decreased 
      melatonin in serum and the expression of AANAT, MT1 and MT2 in placental tissues, 
      elevated the oxidative stress products 8-hydroxy-deoxyguanosine (8-OHdG) and 
      malondialdehyde(MDA) in serum and 4-hydroxynonenal (4HNE) in the placenta, and 
      decreased the antioxidants superoxide dismutase (SOD) and total antioxidant 
      capacity (T-AOC) in serum. Serum proinflammatory cytokines including 
      interleukin-1-beta (IL-1beta), interleukin-6 (IL-6), necrotizing factor-alpha 
      (TNF-alpha), and interleukin-8(IL-8), were all elevated by sleep deprivation, and the 
      inflammatory regulatory factor nuclear factor-kappaB p65 (NF-kappaB p65) in the placenta 
      was enhanced when examined by immunohistochemistry. Corticosterone levels were 
      comparable between the two groups, although ACTH levels were elevated 
      significantly in the sleep deprivation group. Our study revealed that sleep 
      deprivation during pregnancy can adversely impact fetal outcomes. Melatonin may 
      play an important role in this pathology through the oxido-inflammatory process.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Li, Jinzhi
AU  - Li J
AD  - Department of Obstetrics and Gynecology, Jinshan Hospital, Fudan University, 
      Shanghai 201508, China.
FAU - Peng, Xiuhua
AU  - Peng X
AD  - Laboratory Animal Center, Public Health Clinical Center, Fudan University, 
      Shanghai 200000, China.
FAU - Zhong, Hequan
AU  - Zhong H
AD  - Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, China.
FAU - Liu, Songping
AU  - Liu S
AD  - Department of Obstetrics and Gynecology, Jinshan Hospital, Fudan University, 
      Shanghai 201508, China.
FAU - Shi, Jimin
AU  - Shi J
AD  - Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, China.
FAU - Zhou, Xiaohui
AU  - Zhou X
AD  - Laboratory Animal Center, Public Health Clinical Center, Fudan University, 
      Shanghai 200000, China. Electronic address: zhouxiaohui@shaphc.org.
FAU - Li, Bing
AU  - Li B
AD  - Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, China. 
      Electronic address: libingbm@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231102
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - JL5DK93RCL (Melatonin)
RN  - W980KJ009P (Corticosterone)
RN  - 0 (Interleukin-6)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
SB  - IM
MH  - Rats
MH  - Pregnancy
MH  - Female
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - *Placenta/metabolism
MH  - Sleep Deprivation/metabolism
MH  - *Melatonin/metabolism/pharmacology
MH  - Corticosterone/metabolism/pharmacology
MH  - Interleukin-6/metabolism
MH  - Fetus
MH  - Adrenocorticotropic Hormone/metabolism/pharmacology
OTO - NOTNLM
OT  - Fetal outcome
OT  - Melatonin
OT  - Pregnancy
OT  - Sleep deprivation
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/11/06 00:41
MHDA- 2023/12/05 12:43
CRDT- 2023/11/05 18:07
PHST- 2023/06/25 00:00 [received]
PHST- 2023/10/26 00:00 [revised]
PHST- 2023/10/30 00:00 [accepted]
PHST- 2023/12/05 12:43 [medline]
PHST- 2023/11/06 00:41 [pubmed]
PHST- 2023/11/05 18:07 [entrez]
AID - S0165-0378(23)00372-8 [pii]
AID - 10.1016/j.jri.2023.104166 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2023 Dec;160:104166. doi: 10.1016/j.jri.2023.104166. Epub 2023 
      Nov 2.