PMID- 37926697
OWN - NLM
STAT- Publisher
LR  - 20231113
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
DP  - 2023 Nov 5
TI  - Analysis of small EV proteomes reveals unique functional protein networks 
      regulated by VAP-A.
PG  - e2300099
LID - 10.1002/pmic.202300099 [doi]
AB  - Extracellular vesicles (EVs) influence cell phenotypes and functions via protein, 
      nucleic acid, and lipid cargoes. EVs are heterogeneous, due to diverse biogenesis 
      mechanisms that remain poorly understood. Our previous study revealed that the 
      endoplasmic reticulum (ER) membrane contact site (MCS) linker protein vesicle 
      associated protein associated protein A (VAP-A) drives biogenesis of a subset of 
      RNA-enriched EVs. Here, we examine the protein content of VAP-A-regulated EVs. 
      Using label-free proteomics, we identified down- and upregulated proteins in 
      small EVs (SEVs) purified from VAP-A knockdown (KD) colon cancer cells. Gene set 
      enrichment analysis (GSEA) of the data revealed protein classes that are 
      differentially sorted to SEVs dependent on VAP-A. Search Tool for the Retrieval 
      of Reciprocity Genes (STRING) protein-protein interaction network analysis of the 
      RNA-binding protein (RBP) gene set identified several RNA functional machineries 
      that are downregulated in VAP-A KD SEVs, including ribosome, spliceosome, mRNA 
      surveillance, and RNA transport proteins. We also observed downregulation of 
      other functionally interacting protein networks, including cadherin-binding, 
      unfolded protein binding, and ATP-dependent proteins.
CI  - (c) 2023 Wiley-VCH GmbH.
FAU - Barman, Bahnisikha
AU  - Barman B
AD  - Department of Cell and Developmental Biology, Vanderbilt University School of 
      Medicine, Nashville, Tennessee, USA.
AD  - Vanderbilt Center for Extracellular Vesicle Research, Vanderbilt University, 
      Nashville, Tennessee, USA.
FAU - Ramirez, Marisol
AU  - Ramirez M
AD  - Department of Biostatistics, Vanderbilt University Medical Center, Nashville, 
      Tennessee, USA.
FAU - Dawson, Toni Renee
AU  - Dawson TR
AD  - Department of Cell and Developmental Biology, Vanderbilt University School of 
      Medicine, Nashville, Tennessee, USA.
AD  - Vanderbilt Center for Extracellular Vesicle Research, Vanderbilt University, 
      Nashville, Tennessee, USA.
FAU - Liu, Qi
AU  - Liu Q
AD  - Department of Biostatistics, Vanderbilt University Medical Center, Nashville, 
      Tennessee, USA.
FAU - Weaver, Alissa M
AU  - Weaver AM
AUID- ORCID: 0000-0002-4096-8636
AD  - Department of Cell and Developmental Biology, Vanderbilt University School of 
      Medicine, Nashville, Tennessee, USA.
AD  - Vanderbilt Center for Extracellular Vesicle Research, Vanderbilt University, 
      Nashville, Tennessee, USA.
AD  - Department of Pathology, Microbiology and Immunology, Vanderbilt University 
      Medical Center, Nashville, Tennessee, USA.
LA  - eng
GR  - P01CA229123/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20231105
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
SB  - IM
UOF - bioRxiv. 2023 Jul 19;:. PMID: 37502906
OTO - NOTNLM
OT  - endoplasmic reticulum
OT  - extracellular vesicles
OT  - gene set enrichment analysis
OT  - membrane contact sites
OT  - vesicle associated protein-associated protein A (VAP-A)
EDAT- 2023/11/06 00:41
MHDA- 2023/11/06 00:41
CRDT- 2023/11/05 22:53
PHST- 2023/10/10 00:00 [revised]
PHST- 2023/07/15 00:00 [received]
PHST- 2023/10/11 00:00 [accepted]
PHST- 2023/11/06 00:41 [medline]
PHST- 2023/11/06 00:41 [pubmed]
PHST- 2023/11/05 22:53 [entrez]
AID - 10.1002/pmic.202300099 [doi]
PST - aheadofprint
SO  - Proteomics. 2023 Nov 5:e2300099. doi: 10.1002/pmic.202300099.