PMID- 37929694
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20240422
IS  - 1748-6963 (Electronic)
IS  - 1743-5889 (Linking)
VI  - 18
IP  - 23
DP  - 2023 Oct
TI  - Caveolin-1-dependent tenascin C inclusion in extracellular vesicles is required 
      to promote breast cancer cell malignancy.
PG  - 1651-1668
LID - 10.2217/nnm-2023-0143 [doi]
AB  - Background: Elevated expression of CAV1 in breast cancer increases tumor 
      progression. Extracellular vesicles (EVs) from CAV1-expressing MDA-MB-231 breast 
      cancer cells contain Tenascin C (TNC), but the relevance of TNC remained to be 
      defined. Methods: EVs were characterized by nanotracking analysis, microscopy and 
      western blotting. The uptake of EVs by cells was studied using flow cytometry. 
      The effects of EVs on breast cancer cells were tested in migration, invasion, 
      colony formation and in vivo assays. Results: EVs were taken up by cells; 
      however, only those containing TNC promoted invasiveness. In vivo, EVs lacking 
      TNC ceased to promote tumor growth. Conclusion: CAV1 and TNC contained in breast 
      cancer cell-derived EVs were identified as proteins that favor progression of 
      breast cancer.
FAU - Campos, America
AU  - Campos A
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Cientifico y Tecnologico de Excelencia Ciencia y Vida, Santiago, 8340148, 
      Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
AD  - Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, 
      G61 1BD, Scotland.
FAU - Burgos-Ravanal, Renato
AU  - Burgos-Ravanal R
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
FAU - Lobos-Gonzalez, Lorena
AU  - Lobos-Gonzalez L
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
AD  - Centro de Medicina Regenerativa, Facultad de Medicina-Clinica Alemana, 
      Universidad del Desarrollo, Santiago, 7610615, Chile.
FAU - Huilcaman, Ricardo
AU  - Huilcaman R
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
FAU - Gonzalez, Maria Fernanda
AU  - Gonzalez MF
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
FAU - Diaz, Jorge
AU  - Diaz J
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
FAU - Verschae, Albano Caceres
AU  - Verschae AC
AD  - Laboratorio de Biologia Celular del Cancer, CEBICEM, Universidad San Sebastian, 
      Santiago, 7510157, Chile.
AD  - Department of Oncology/Pathology, Karolinska Institutet, Stockholm, 17177, 
      Sweden.
FAU - Acevedo, Juan Pablo
AU  - Acevedo JP
AD  - Center of Interventional Medicine for Precision & Advanced Cellular Therapy 
      (IMPACT), Santiago, 8331150, Chile.
FAU - Carrasco, Macarena
AU  - Carrasco M
AD  - Centro Cientifico y Tecnologico de Excelencia Ciencia y Vida, Santiago, 8340148, 
      Chile.
FAU - Sepulveda, Francisca
AU  - Sepulveda F
AD  - Centro Cientifico y Tecnologico de Excelencia Ciencia y Vida, Santiago, 8340148, 
      Chile.
AD  - Centro de Medicina Regenerativa, Facultad de Medicina-Clinica Alemana, 
      Universidad del Desarrollo, Santiago, 7610615, Chile.
FAU - Jeldes, Emanuel
AU  - Jeldes E
AD  - Centro Cientifico y Tecnologico de Excelencia Ciencia y Vida, Santiago, 8340148, 
      Chile.
AD  - Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, 
      G61 1BD, Scotland.
FAU - Varas-Godoy, Manuel
AU  - Varas-Godoy M
AD  - Centro Cientifico y Tecnologico de Excelencia Ciencia y Vida, Santiago, 8340148, 
      Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
AD  - Laboratorio de Biologia Celular del Cancer, CEBICEM, Universidad San Sebastian, 
      Santiago, 7510157, Chile.
FAU - Leyton, Lisette
AU  - Leyton L
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
FAU - Quest, Andrew Fg
AU  - Quest AF
AUID- ORCID: 0000-0001-8755-1590
AD  - Laboratorio de Comunicaciones Celulares, Centro de Estudios en Ejercicio, 
      Metabolismo y Cancer (CEMC), Programa de Biologia Celular y Molecular, Facultad 
      de Medicina, 8380492, Universidad de Chile.
AD  - Centro Avanzado para Estudios en Enfermedades Cronicas (ACCDIS), Santiago, 
      8380492, Chile.
LA  - eng
GR  - 21130102, 21161246, 21170292, 21200147/ANID PhD fellowship/
GR  - 1190928, 1200836, 1210644, 1211223/Fondo Nacional de Desarrollo Cientifico y 
      Tecnologico/
GR  - 15130011/Fondo de Financiamiento de Centros de Investigacion en Areas 
      Prioritarias/
GR  - FB210008, FB210024/ANID BASAL/
GR  - 3170169/ANID postdoctoral/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231106
PL  - England
TA  - Nanomedicine (Lond)
JT  - Nanomedicine (London, England)
JID - 101278111
RN  - 0 (CAV1 protein, human)
RN  - 0 (Caveolin 1)
RN  - 0 (TNC protein, human)
RN  - 0 (Tenascin)
SB  - IM
MH  - Humans
MH  - Cell Line, Tumor
MH  - *Breast Neoplasms/metabolism/pathology
MH  - *Caveolin 1/metabolism
MH  - *Extracellular Vesicles/metabolism
MH  - *Tenascin/metabolism
MH  - Animals
MH  - Mice
MH  - Mice, SCID
MH  - Disease Progression
OAB - Caveolin-1 (CAV1) is a protein that in breast cancer increases with disease 
      progression. Extracellular vesicles (EVs) from breast cancer cells with CAV1 also 
      contain Tenascin C (TNC) protein, but the importance of TNC remained to be 
      defined. EVs were identified by size, microscopy and protein analysis. The 
      effects of EVs on breast cancer cells were studied using cells and experiments in 
      animals. CAV1 expression promotes TNC inclusion into EVs, which increased the 
      aggressiveness of recipient breast cancer cells. In animals, only EVs with TNC 
      increased features associated with cancer spread, while EVs lacking TNC reduced 
      tumor growth.
OABL- eng
OTO - NOTNLM
OT  - CAV1
OT  - TNC
OT  - breast cancer
OT  - exosomes
OT  - metastasis
EDAT- 2023/11/06 06:42
MHDA- 2023/11/06 06:43
CRDT- 2023/11/06 05:52
PHST- 2023/11/06 06:43 [medline]
PHST- 2023/11/06 06:42 [pubmed]
PHST- 2023/11/06 05:52 [entrez]
AID - 10.2217/nnm-2023-0143 [doi]
PST - ppublish
SO  - Nanomedicine (Lond). 2023 Oct;18(23):1651-1668. doi: 10.2217/nnm-2023-0143. Epub 
      2023 Nov 6.