PMID- 37930731
OWN - NLM
STAT- MEDLINE
DCOM- 20240219
LR  - 20240220
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 30
IP  - 3-4
DP  - 2024 Feb
TI  - LATS1/YAP1 Axis Controls Bone Regeneration on Distraction Osteogenesis by 
      Activating Wnt/beta-Catenin.
PG  - 154-167
LID - 10.1089/ten.TEA.2023.0091 [doi]
AB  - The Hippo signaling pathway inhibits cell growth, and its components and 
      functions are highly conserved in mammals. LATS1 is a core component of the Hippo 
      signaling pathway associated with lymphatic invasion, astrogliosis, apoptosis, 
      and autophagy. Nevertheless, the role of Hippo/LATS1 in osteogenesis remains 
      unclear. In this study, we used ribonucleic acid (RNA) lentiviruses to inhibit 
      the expression of Lats1 in bone marrow-derived stem cells (BMSCs) and distraction 
      osteogenic regions in rats. Increased osteogenic, proliferative, and migratory 
      abilities of BMSCs were observed in Lats1-inhibited BMSCs, while these phenotypes 
      were partially reversed by YAP1 inhibition. In vivo, we found that the LATS1/YAP1 
      axis promoted osteogenesis during distraction osteogenesis (DO). beta-catenin was 
      positively correlated with YAP1 expression in vivo and in vitro. When YAP1 was 
      strongly positive in the nucleus, beta-catenin expression was upregulated; when YAP1 
      expression was inhibited by verteporfin, beta-catenin was not expressed in the 
      nucleus. These findings suggest that the LATS1/YAP1 signaling axis promotes DO by 
      activating the Wnt/beta-catenin signaling pathway. This study provides insights into 
      the molecular mechanism of osteogenesis and a potential therapeutic strategy for 
      bone regeneration in DO by associating with LATS1/YAP1-beta-catenin.
FAU - Li, Kehan
AU  - Li K
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
AD  - National Center for Stomatology, National Clinical Research Center for Oral 
      Disease, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of 
      Stomatology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Liu, Linan
AU  - Liu L
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
FAU - Liu, Hanghang
AU  - Liu H
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
FAU - Xing, Jiawei
AU  - Xing J
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
FAU - Hu, Pei
AU  - Hu P
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
FAU - Song, Jian
AU  - Song J
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      China.
LA  - eng
GR  - 81800948 AND 82001018/National Natural Science Foundation of China/
GR  - 19PJ232/Research On The Subject of the Health Commission of Sichuan Province/
GR  - 22NSFSC2628/Natural Science Foundation of Sichuan Province/
PT  - Journal Article
DEP - 20231214
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Rats
MH  - Animals
MH  - beta Catenin/metabolism
MH  - Cell Differentiation
MH  - *Osteogenesis, Distraction
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Osteogenesis/genetics
MH  - Wnt Signaling Pathway
MH  - Bone Regeneration
MH  - Cells, Cultured
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Hippo signaling
OT  - Wnt/beta-catenin signaling
OT  - YAP1
OT  - distraction osteogenesis
OT  - lentivirus
EDAT- 2023/11/06 12:43
MHDA- 2024/02/19 06:44
CRDT- 2023/11/06 11:43
PHST- 2024/02/19 06:44 [medline]
PHST- 2023/11/06 12:43 [pubmed]
PHST- 2023/11/06 11:43 [entrez]
AID - 10.1089/ten.TEA.2023.0091 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2024 Feb;30(3-4):154-167. doi: 10.1089/ten.TEA.2023.0091. Epub 
      2023 Dec 14.