PMID- 37932697 OWN - NLM STAT- MEDLINE DCOM- 20231108 LR - 20231110 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 24 IP - 1 DP - 2023 Nov 6 TI - Differential expressions of FASN, SCD, and FABP4 genes in the ribeye muscle of omega-3 oil-supplemented Tattykeel Australian White lambs. PG - 666 LID - 10.1186/s12864-023-09771-x [doi] LID - 666 AB - BACKGROUND: The concept of the functional nutritional value of health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) is becoming a phenomenon among red meat consumers globally. This study examined the expressions of three lipogenic genes (fatty acid binding protein 4, FABP4, fatty acid synthase, FASN; and stearoyl-CoA desaturase, SCD) in the ribeye (Longissimus thoracis et lumborum) muscle of Tattykeel Australian White (TAW) lambs fed fortified omega-3 diets and correlations with fatty acids. To answer the research question, "are there differences in the expression of lipogenic genes between control, MSM whole grain and omega-3 supplemented lambs?", we tested the hypothesis that fortification of lamb diets with omega-3 will lead to a down-regulation of lipogenic genes. Seventy-five six-month old TAW lambs were randomly allocated to the (1) omega-3 oil-fortified grain pellets, (2) unfortified grain pellets (control) or (3) unfortified MSM whole grain pellets diet supplements to generate three treatments of 25 lambs each. The feeding trial lasted 47 days. RESULTS: From the Kruskal-Wallis test, the results showed a striking disparity in lipogenic gene expression between the three dietary treatments in which the FABP4 gene was significantly up-regulated by 3-folds in the muscles of lambs fed MSM Milling (MSM) whole grain diet compared to the omega-3 and control diets. A negative correlation was observed between FASN gene expression and intramuscular fat (IMF), eicosapentaenoic acid (EPA), total polyunsaturated fatty acids (PUFA), omega-6 polyunsaturated fatty acids (n-6 PUFA) and monounsaturated fatty acids (MUFA). The FABP4 gene expression was positively correlated (P < 0.05) with EPA and docosahexaenoic acid (DHA). CONCLUSION: Taken together, this study's results suggest that FABP4 and FASN genes perform an important role in the biosynthesis of fatty acids in the ribeye muscle of TAW lambs, and supplementary diet composition is an important factor influencing their expressions. CI - (c) 2023. The Author(s). FAU - Otto, John Roger AU - Otto JR AD - School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia. ajulu04@gmail.com. FAU - Pewan, Shedrach Benjamin AU - Pewan SB AD - School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia. AD - National Veterinary Research Institute, Private Mail Bag 01, Vom, Plateau State, Nigeria. FAU - Edmunds, Richard Crawford AU - Edmunds RC AD - Australian Institute of Marine Science, Townsville, QLD, 4810, Australia. FAU - Mwangi, Felista Waithira AU - Mwangi FW AD - School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia. AD - School of Medicine and Public Health, College of Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, NSW, 2308, Australia. FAU - Kinobe, Robert Tumwesigye AU - Kinobe RT AD - College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia. FAU - Adegboye, Oyelola Abdulwasiu AU - Adegboye OA AD - Menzies School of Health Research, Charles Darwin University, Casuarina, NT, 0811, Australia. FAU - Malau-Aduli, Aduli Enoch Othniel AU - Malau-Aduli AEO AD - School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia. LA - eng GR - ICG001084/The Innovation Connections Research Grant from the Australian Commonwealth Government's Department of Industry/ GR - ICG001084/The Innovation Connections Research Grant from the Australian Commonwealth Government's Department of Industry/ GR - 00044/Science Industry Endowment Fund Ross Metcalf STEM Business Fellowship co-funded by Tattykeel Australian White Pty Ltd/ GR - 00044/Science Industry Endowment Fund Ross Metcalf STEM Business Fellowship co-funded by Tattykeel Australian White Pty Ltd/ PT - Journal Article DEP - 20231106 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 RN - EC 1.14.19.1 (Stearoyl-CoA Desaturase) RN - 0 (Fatty Acids, Omega-3) RN - 0 (Fatty Acids, Unsaturated) RN - 0 (Fatty Acids) RN - AAN7QOV9EA (Eicosapentaenoic Acid) RN - 0 (Transcription Factors) SB - IM MH - Sheep MH - Animals MH - *Stearoyl-CoA Desaturase MH - Australia MH - *Fatty Acids, Omega-3/metabolism MH - Fatty Acids, Unsaturated MH - Fatty Acids/metabolism MH - Sheep, Domestic MH - Diet/veterinary MH - Eicosapentaenoic Acid MH - Transcription Factors/genetics MH - Muscles/metabolism PMC - PMC10626737 OTO - NOTNLM OT - Fatty acid binding protein 4 OT - Fatty acid synthase OT - Omega-3 fortified diets OT - Ribeye muscle OT - Stearoyl-CoA desaturase OT - Tattykeel Australian White OT - Unsaturated fatty acids COIS- The authors declare no competing interests. EDAT- 2023/11/07 00:42 MHDA- 2023/11/08 06:42 PMCR- 2023/11/06 CRDT- 2023/11/06 23:53 PHST- 2023/05/07 00:00 [received] PHST- 2023/10/27 00:00 [accepted] PHST- 2023/11/08 06:42 [medline] PHST- 2023/11/07 00:42 [pubmed] PHST- 2023/11/06 23:53 [entrez] PHST- 2023/11/06 00:00 [pmc-release] AID - 10.1186/s12864-023-09771-x [pii] AID - 9771 [pii] AID - 10.1186/s12864-023-09771-x [doi] PST - epublish SO - BMC Genomics. 2023 Nov 6;24(1):666. doi: 10.1186/s12864-023-09771-x.