PMID- 37935360
OWN - NLM
STAT- MEDLINE
DCOM- 20231109
LR  - 20231229
IS  - 1742-5662 (Electronic)
IS  - 1742-5689 (Print)
IS  - 1742-5662 (Linking)
VI  - 20
IP  - 208
DP  - 2023 Nov
TI  - Red edge excitation shift spectroscopy is highly sensitive to tryptophan 
      composition.
PG  - 20230337
LID - 10.1098/rsif.2023.0337 [doi]
LID - 20230337
AB  - Red edge excitation shift (REES) spectroscopy relies on the unique emission 
      profiles of fluorophore-solvent interactions to profile protein molecular 
      dynamics. Recently, we reported the use of REES to compare the stability of 32 
      polymorphic IgG antibodies natively containing tryptophan reporter fluorophores. 
      Here, we expand on this work to investigate the sensitivity of REES to variations 
      in tryptophan content using a subset of IgG3 antibodies containing arginine to 
      tryptophan polymorphisms. Structural analysis revealed that the additional 
      tryptophan residues were situated in highly solvated environments. Subsequently, 
      REES showed clear differences in fluorescence emission profiles when compared 
      with the unmutated variants, thereby limiting direct comparison of their 
      structural dynamics. These findings highlight the exquisite sensitivity of REES 
      to minor variations in protein structure and tryptophan composition.
FAU - Warrender, Annmaree K
AU  - Warrender AK
AD  - Te Huataki Waiora School of Health, University of Waikato, Hamilton, New Zealand.
FAU - Pan, Jolyn
AU  - Pan J
AD  - Te Aka Matuatua School of Science, University of Waikato, Hamilton, New Zealand.
FAU - Pudney, Chris
AU  - Pudney C
AD  - Department of Biology and Biochemistry, University of Bath, Bath, UK.
FAU - Arcus, Vickery L
AU  - Arcus VL
AUID- ORCID: 0000-0001-5082-2414
AD  - Te Aka Matuatua School of Science, University of Waikato, Hamilton, New Zealand.
FAU - Kelton, William
AU  - Kelton W
AUID- ORCID: 0000-0002-5513-6429
AD  - Te Huataki Waiora School of Health, University of Waikato, Hamilton, New Zealand.
AD  - Te Aka Matuatua School of Science, University of Waikato, Hamilton, New Zealand.
LA  - eng
SI  - figshare/10.6084/m9.figshare. c.6908880
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231108
PL  - England
TA  - J R Soc Interface
JT  - Journal of the Royal Society, Interface
JID - 101217269
RN  - 8DUH1N11BX (Tryptophan)
RN  - 0 (Proteins)
SB  - IM
MH  - *Tryptophan/chemistry
MH  - Spectrometry, Fluorescence/methods
MH  - *Proteins
PMC - PMC10645072
OTO - NOTNLM
OT  - antibodies
OT  - protein dynamics
OT  - protein stability
OT  - red edge excitation shift
OT  - tryptophan fluorescence
COIS- C.P. is a director of BLOC Laboratories Ltd, which holds a relevant patent 
      (WO2017158371A1).
EDAT- 2023/11/08 00:41
MHDA- 2023/11/09 06:42
PMCR- 2023/11/08
CRDT- 2023/11/07 19:14
PHST- 2023/11/09 06:42 [medline]
PHST- 2023/11/08 00:41 [pubmed]
PHST- 2023/11/07 19:14 [entrez]
PHST- 2023/11/08 00:00 [pmc-release]
AID - rsif20230337 [pii]
AID - 10.1098/rsif.2023.0337 [doi]
PST - ppublish
SO  - J R Soc Interface. 2023 Nov;20(208):20230337. doi: 10.1098/rsif.2023.0337. Epub 
      2023 Nov 8.