PMID- 37935388
OWN - NLM
STAT- MEDLINE
DCOM- 20231211
LR  - 20231216
IS  - 1439-3646 (Electronic)
IS  - 0947-7349 (Print)
IS  - 0947-7349 (Linking)
VI  - 131
IP  - 12
DP  - 2023 Dec
TI  - Vitamin D Alleviates Type 2 Diabetes Mellitus by Mitigating Oxidative 
      Stress-Induced Pancreatic beta-Cell Impairment.
PG  - 656-666
LID - 10.1055/a-2191-9969 [doi]
AB  - OBJECTIVE: Type 2 diabetes mellitus (T2DM) is a common metabolic disorder with 
      rising incidence worldwide. This study explored the anti-T2DM role of vitamin D, 
      thereby providing novel therapeutic strategies. METHODS: C57BL/6 J mice and MIN6 
      cells were used to induce in vivo T2DM and damaged beta-cell models, respectively. 
      Body weights, fasting blood glucose, and fasting insulin were measured in mice. 
      Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were 
      conducted on mice. Lipid indices (TG, TC, LDL-C, and HDL-C) were detected in 
      mouse serum. Hematoxylin-eosin staining was used to evaluate pancreatic tissue 
      injury. ELISA was used to assess insulin and oxidative stress (OS) markers (MDA, 
      GSH, and SOD) in mice and MIN6 cells. Production of ROS was detected in islet 
      beta-cells and MIN6 cells. Cell viability and apoptosis were evaluated using CCK-8 
      and flow cytometry, respectively. QRT-PCR and western blotting were used to 
      detect pro-inflammatory factors (TNF-alpha and IL-6) and endoplasmic reticulum stress 
      (ERS) markers (CHOP and GRP78), respectively. RESULTS: Vitamin D reduced body 
      weights, fasting blood glucose, and insulin and ameliorated glucose tolerance and 
      insulin sensitivity in T2DM mice. Besides, vitamin D decreased serum TG, TC, 
      LDL-C, and increased HDL-C in T2DM mice. Vitamin D inhibited pancreatic 
      histopathological injury, cell apoptosis, OS, and beta-cell decline in T2DM mice. 
      Moreover, vitamin D alleviated cell death, insufficient insulin secretion, 
      inflammation, OS, and ERS in damaged MIN6 cells. Notably, N-acetyl-L-cysteine (an 
      OS inhibitor) enhanced these effects of vitamin D. CONCLUSIONS: Vitamin D 
      relieved T2DM symptoms by alleviating OS-induced beta-cell impairment.
CI  - The Author(s). This is an open access article published by Thieme under the terms 
      of the Creative Commons Attribution-NonDerivative-NonCommercial-License, 
      permitting copying and reproduction so long as the original work is given 
      appropriate credit. Contents may not be used for commercial purposes, or adapted, 
      remixed, transformed or built upon. 
      (https://creativecommons.org/licenses/by-nc-nd/4.0/).
FAU - Liu, Jia
AU  - Liu J
AD  - Department of Endocrinology, Gansu Provincial Hospital, Lanzhou, Gansu, China.
FAU - Zhang, Yuanjun
AU  - Zhang Y
AD  - Department of Endocrinology, Gansu Provincial Hospital, Lanzhou, Gansu, China.
FAU - Shi, Derong
AU  - Shi D
AD  - Gansu University of Chinese Medicine, Gansu, China.
FAU - He, Cuihuan
AU  - He C
AD  - Gansu University of Chinese Medicine, Gansu, China.
FAU - Xia, Guanghao
AU  - Xia G
AD  - Department of Endocrinology, Gansu Provincial Hospital, Lanzhou, Gansu, China.
LA  - eng
PT  - Journal Article
DEP - 20231107
PL  - Germany
TA  - Exp Clin Endocrinol Diabetes
JT  - Experimental and clinical endocrinology & diabetes : official journal, German 
      Society of Endocrinology [and] German Diabetes Association
JID - 9505926
RN  - 0 (Blood Glucose)
RN  - 1406-16-2 (Vitamin D)
RN  - 0 (Cholesterol, LDL)
RN  - 0 (Insulin)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Blood Glucose/metabolism
MH  - Vitamin D/pharmacology/therapeutic use
MH  - Cholesterol, LDL
MH  - Mice, Inbred C57BL
MH  - Insulin
MH  - *Insulin-Secreting Cells/metabolism
MH  - Oxidative Stress
MH  - Body Weight
PMC - PMC10700019
COIS- The authors declare that they have no conflict of interest.
EDAT- 2023/11/08 00:42
MHDA- 2023/12/11 12:42
PMCR- 2023/11/01
CRDT- 2023/11/07 19:22
PHST- 2023/12/11 12:42 [medline]
PHST- 2023/11/08 00:42 [pubmed]
PHST- 2023/11/07 19:22 [entrez]
PHST- 2023/11/01 00:00 [pmc-release]
AID - eced06-2023-0182-dia [pii]
AID - 10.1055/a-2191-9969 [doi]
PST - ppublish
SO  - Exp Clin Endocrinol Diabetes. 2023 Dec;131(12):656-666. doi: 10.1055/a-2191-9969. 
      Epub 2023 Nov 7.