PMID- 37936947
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231109
IS  - 2517-729X (Print)
IS  - 2517-729X (Electronic)
IS  - 2517-729X (Linking)
VI  - 36
IP  - 5
DP  - 2023
TI  - Single-nucleus transcriptome profiling of prefrontal cortex induced by chronic 
      methamphetamine treatment.
PG  - e101057
LID - 10.1136/gpsych-2023-101057 [doi]
LID - e101057
AB  - BACKGROUND: Methamphetamine (METH) addiction causes a huge burden on society. The 
      prefrontal cortex (PFC), associated with emotion and cognitive behaviours, is 
      also involved in addiction neurocircuitry. Although bulk RNA sequencing has shown 
      METH-induced gene alterations in the mouse PFC, the impact on different cell 
      types remains unknown. AIMS: To clarify the effects of METH treatment on 
      different cell types of the PFC and the potential pathways involved in 
      METH-related disorders. METHODS: We performed single-nucleus RNA sequencing 
      (snRNA-seq) to examine the transcriptomes of 20 465 nuclei isolated from the PFC 
      of chronic METH-treated and control mice. Main cell types and differentially 
      expressed genes (DEGs) were identified and confirmed by RNA fluorescence in situ 
      hybridization(FISH). RESULTS: Six main cell types were identified depending on 
      the single-cell nucleus sequencing; of particular interest were the mature 
      oligodendrocytes in the PFC. The DEGs of mature oligodendrocytes were enriched in 
      the myelin sheath, adenosine triphosphate (ATP) metabolic process, mitochondrial 
      function and components, and so on. The messenger RNA levels of Aldoc and Atp5l 
      (FISH) and the protein level of the mitochondrial membrane pore subunit TOM40 
      (immunofluorescence) decreased in the mature oligodendrocytes. Fast blue staining 
      and transmission electron microscopy image indicated myelin damage, and the 
      myelin thickness decreased in METH brains. CONCLUSIONS: snRNA-seq reveals altered 
      transcriptomes of different cell types in mouse PFC induced by chronic METH 
      treatment, underscoring potential relationships with psychiatric disorders.
CI  - (c) Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No 
      commercial re-use. See rights and permissions. Published by BMJ.
FAU - Zeng, Kuan
AU  - Zeng K
AD  - Department of Psychiatry, Wuhan Mental Health Center, Wuhan, Hubei, China.
AD  - Research Center for Psychological and Health Sciences, China University of 
      Geosciences, Wuhan, Hubei, China.
FAU - Yu, Xuan
AU  - Yu X
AD  - Department of Pathophysiology, School of Basic Medicine, Key Laboratory of 
      Education Ministry/Hubei Province of China for Neurological Disorders, Huazhong 
      University of Science and Technology Tongji Medical College, Wuhan, Hubei, China.
FAU - Wei, Zhen
AU  - Wei Z
AD  - Department of Pathophysiology, School of Basic Medicine, Key Laboratory of 
      Education Ministry/Hubei Province of China for Neurological Disorders, Huazhong 
      University of Science and Technology Tongji Medical College, Wuhan, Hubei, China.
AD  - Department of Pathology, Maternal and Child Hospital of Hubei Province, Huazhong 
      University of Science and Technology Tongji Medical College, Wuhan, Hubei, China.
FAU - Wu, Yong
AU  - Wu Y
AD  - Department of Psychiatry, Wuhan Mental Health Center, Wuhan, Hubei, China.
AD  - Research Center for Psychological and Health Sciences, China University of 
      Geosciences, Wuhan, Hubei, China.
FAU - Wang, Jianzhi
AU  - Wang J
AD  - Department of Pathophysiology, School of Basic Medicine, Key Laboratory of 
      Education Ministry/Hubei Province of China for Neurological Disorders, Huazhong 
      University of Science and Technology Tongji Medical College, Wuhan, Hubei, China.
AD  - Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 
      China.
FAU - Liu, Rong
AU  - Liu R
AD  - Department of Pathophysiology, School of Basic Medicine, Key Laboratory of 
      Education Ministry/Hubei Province of China for Neurological Disorders, Huazhong 
      University of Science and Technology Tongji Medical College, Wuhan, Hubei, China.
FAU - Li, Yi
AU  - Li Y
AUID- ORCID: 0000-0002-8876-0041
AD  - Department of Psychiatry, Wuhan Mental Health Center, Wuhan, Hubei, China.
AD  - Research Center for Psychological and Health Sciences, China University of 
      Geosciences, Wuhan, Hubei, China.
FAU - Wang, Xiaochuan
AU  - Wang X
AUID- ORCID: 0000-0001-8207-0042
AD  - Department of Pathophysiology, School of Basic Medicine, Key Laboratory of 
      Education Ministry/Hubei Province of China for Neurological Disorders, Huazhong 
      University of Science and Technology Tongji Medical College, Wuhan, Hubei, China.
AD  - Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20231102
PL  - England
TA  - Gen Psychiatr
JT  - General psychiatry
JID - 101735271
PMC - PMC10626793
OTO - NOTNLM
OT  - Behavior, Addictive
OT  - Brain
OT  - Central Nervous System
OT  - Prefrontal Cortex
COIS- Competing interests: None declared.
EDAT- 2023/11/08 06:43
MHDA- 2023/11/08 06:44
PMCR- 2023/11/02
CRDT- 2023/11/08 03:57
PHST- 2023/03/26 00:00 [received]
PHST- 2023/10/01 00:00 [accepted]
PHST- 2023/11/08 06:44 [medline]
PHST- 2023/11/08 06:43 [pubmed]
PHST- 2023/11/08 03:57 [entrez]
PHST- 2023/11/02 00:00 [pmc-release]
AID - gpsych-2023-101057 [pii]
AID - 10.1136/gpsych-2023-101057 [doi]
PST - epublish
SO  - Gen Psychiatr. 2023 Nov 2;36(5):e101057. doi: 10.1136/gpsych-2023-101057. 
      eCollection 2023.