PMID- 37941784
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231110
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 18
IP  - 1
DP  - 2023
TI  - PTX3 promotes IVIG resistance-induced endothelial injury in Kawasaki disease by 
      regulating the NF-kappaB pathway.
PG  - 20220735
LID - 10.1515/biol-2022-0735 [doi]
LID - 20220735
AB  - Intravenous immunoglobulin (IVIG) resistance leads to serious complications in 
      Kawasaki disease (KD) with no effective treatment. This study aimed to 
      investigate the effects of pentraxin 3 (PTX3) on human coronary artery 
      endothelial cells (HCAECs). PTX3 levels were measured using quantitative 
      real-time PCR (qRT-PCR), enzyme-linked immunosorbent assay, and western blotting. 
      Cell viability was detected using the MTT assay. Biological functions were 
      analyzed using CCK-8, EdU, flow cytometry, TUNEL, and qRT-PCR. The levels of 
      factors of the NF-kappaB pathway were examined using western blotting. The results 
      demonstrated that PTX3 expression was highest in patients and HCAECs with 
      IVIG-resistance. Knockdown of PTX3 promoted proliferation and suppressed 
      apoptosis and inflammation of IVIG-resistant HCAECs, whereas PTX3 overexpression 
      produced the opposite results. Moreover, PTX3 activated the NF-kappaB pathway in 
      IVIG-resistant HCAECs. A rescue study showed that PTX3 modulated biological 
      behaviors by regulating the NF-kappaB pathway. Overall, our findings demonstrate that 
      PTX3 promotes IVIG resistance-induced endothelial injury by activating the NF-kappaB 
      pathway, suggesting that PTX3 may become a novel therapeutic target for patients 
      with IVIG-resistant KD.
CI  - (c) 2023 the author(s), published by De Gruyter.
FAU - Sun, Ye
AU  - Sun Y
AD  - Children's Hospital of Shanxi (Women Health Center of Shanxi), No. 65, Jinxi 
      Street, Taiyuan, Shanxi 030025, China.
FAU - Liu, Lihua
AU  - Liu L
AD  - Children's Hospital of Shanxi (Women Health Center of Shanxi), No. 65, Jinxi 
      Street, Taiyuan, Shanxi 030025, China.
FAU - Yang, Ruihua
AU  - Yang R
AD  - Children's Hospital of Shanxi (Women Health Center of Shanxi), No. 65, Jinxi 
      Street, Taiyuan, Shanxi 030025, China.
LA  - eng
PT  - Journal Article
DEP - 20231024
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC10628575
OTO - NOTNLM
OT  - IVIG resistance
OT  - Kawasaki disease
OT  - NF-kappaB pathway
OT  - PTX3
OT  - coronary artery endothelial cells
COIS- Conflict of interest: Authors state no conflict of interest.
EDAT- 2023/11/09 06:41
MHDA- 2023/11/09 06:42
PMCR- 2023/10/24
CRDT- 2023/11/09 04:12
PHST- 2023/06/12 00:00 [received]
PHST- 2023/08/20 00:00 [revised]
PHST- 2023/08/29 00:00 [accepted]
PHST- 2023/11/09 06:42 [medline]
PHST- 2023/11/09 06:41 [pubmed]
PHST- 2023/11/09 04:12 [entrez]
PHST- 2023/10/24 00:00 [pmc-release]
AID - biol-2022-0735 [pii]
AID - 10.1515/biol-2022-0735 [doi]
PST - epublish
SO  - Open Life Sci. 2023 Oct 24;18(1):20220735. doi: 10.1515/biol-2022-0735. 
      eCollection 2023.