PMID- 37947356
OWN - NLM
STAT- MEDLINE
DCOM- 20231129
LR  - 20231205
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Linking)
VI  - 17
IP  - 22
DP  - 2023 Nov 28
TI  - Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the 
      Activation of Senescence-Associated Secretory Phenotype.
PG  - 22885-22900
LID - 10.1021/acsnano.3c07807 [doi]
AB  - Stem cell senescence is one of the most representative events of organism aging 
      and is responsible for many physiological abnormalities and disorders. In the 
      scenario of orthopedic disease treatment, stem cell aging may affect the 
      implantation outcome and even lead to operation failure. To explore whether stem 
      cell aging will affect the osteointegration effect of titanium implant, a widely 
      used micronano titanium (MNT) was fabricated. We first verified the expected 
      osteointegration effect of the MNT, which could be attributed to the improvement 
      of stem cell adhesion and osteogenic differentiation. Then, we obtained 
      aged-derived bone marrow mesenchymal stem cells (BMSCs) and studied their 
      biological behaviors on MNT both in vitro and in vivo. We found that compared 
      with normal rats, MNT did not significantly improve the osteointegration in aged 
      rats. Compared with normal rats, fewer endogenous stem cells were observed at the 
      implant-host interface, and the expression of p21 (senescence marker) was also 
      higher. We further confirmed that MNT promoted the nuclear localization of NF-kappaB 
      in senescent stem cells through the activation of p38 MAPK, thereby inducing the 
      occurrence of the senescence-associated secretory phenotype (SASP) and ultimately 
      leading to the depletion of the stem-cell pool at the implant-host interface. 
      However, the activation of p38 MAPK can still promote the osteogenic 
      differentiation of nonsenescent BMSCs. These results showed an interesting 
      paradoxical balance between osteogenesis and senescence on MNT surfaces and also 
      provided insights for the design of orthopedic implants for aging patients.
FAU - Li, Xuan
AU  - Li X
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Luo, Xinxin
AU  - Luo X
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - He, Ye
AU  - He Y
AD  - Thomas Lord Department of Mechanical Engineering and Materials Science, Duke 
      University, Durham, North Carolina 27708, United States.
FAU - Xu, Kun
AU  - Xu K
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Ding, Yao
AU  - Ding Y
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Gao, Pengfei
AU  - Gao P
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Tao, Bailong
AU  - Tao B
AD  - Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, P. R. China.
FAU - Li, Meng
AU  - Li M
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Tan, Meijun
AU  - Tan M
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Liu, Shaopeng
AU  - Liu S
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Liu, Peng
AU  - Liu P
AUID- ORCID: 0000-0001-9406-6024
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
FAU - Cai, Kaiyong
AU  - Cai K
AUID- ORCID: 0000-0001-9029-680X
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education 
      College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231110
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - D1JT611TNE (Titanium)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Rats
MH  - Humans
MH  - Animals
MH  - Aged
MH  - *Titanium/pharmacology/metabolism
MH  - Senescence-Associated Secretory Phenotype
MH  - Osteogenesis
MH  - Cell Differentiation
MH  - *Mesenchymal Stem Cells
MH  - p38 Mitogen-Activated Protein Kinases/metabolism/pharmacology
MH  - Cells, Cultured
OTO - NOTNLM
OT  - mesenchymal stem cells
OT  - micronano structure
OT  - osteointegration
OT  - senescence
OT  - titanium implant
EDAT- 2023/11/10 12:44
MHDA- 2023/11/29 06:43
CRDT- 2023/11/10 07:53
PHST- 2023/11/29 06:43 [medline]
PHST- 2023/11/10 12:44 [pubmed]
PHST- 2023/11/10 07:53 [entrez]
AID - 10.1021/acsnano.3c07807 [doi]
PST - ppublish
SO  - ACS Nano. 2023 Nov 28;17(22):22885-22900. doi: 10.1021/acsnano.3c07807. Epub 2023 
      Nov 10.