PMID- 37951404 OWN - NLM STAT- MEDLINE DCOM- 20231206 LR - 20231217 IS - 1879-1298 (Electronic) IS - 0045-6535 (Linking) VI - 347 DP - 2024 Jan TI - Single cell ICP-MS to evaluate the interaction behaviour for Cd, Ce and U with Streptomyces coelicolor spores. PG - 140633 LID - S0045-6535(23)02903-X [pii] LID - 10.1016/j.chemosphere.2023.140633 [doi] AB - Streptomyces are important soil bacteria used for bioremediation of metal-contaminated soils, however, it is still unknown how metal-selective Streptomyces are and which mechanisms are involved during their capture. In this work, we exposed S. coelicolor spores to environmentally relevant concentrations (0.1, 1, 10, 100 muM) of Ce, U and Cd in solid medium for one week to investigate the uptake behaviour of hyphae in the newly formed spores. Additionally, metal adsorption onto the spores was explored by incubating inactive, ungerminated spores for one day in aqueous metal solution. The spore-washing treatment was key to distinguishing between strongly spore-associated (e.g. incorporation; Tris-EDTA buffer) and weakly spore-associated metals (Tris buffer alone minus Tris-EDTA). Single cell (sc) ICP-MS was used to quantify metal-associated content in individual spores. Our results revealed element-specific adsorption onto inactive spores showing that out of the total metal exposure, both strongly (Ce: 58%; U: 54%; Cd: 28%) and weakly (Ce: 12%; U: 1%; Cd: 18%) adsorbed metals occur. However, scICP-MS showed that from metal-amended solid medium, only Ce and U were strongly spore-associated (averages 0.040 and 0.062 fg spore(-1) for 10 muM exposures, respectively) while Cd was below the limit of detection (< 0.006 fg spore(-1)). We propose that hyphae only metabolically interact with Ce in a controlled manner but uncontrolled with U, as 66-73% Ce and only 2-4% U were inherited from adsorbed content. We conclude that Streptomyces spore-metal interaction starts with a relevant adsorption step of Ce, U and Cd as presented for aqueous conditions. If spores start to germinate, hyphae are capable of effectively encapsulating Ce and U, but not Cd. This study brings light into the still unknown field of metal interactions with Streptomyces and applied understanding for more efficient and metal-specific use of Streptomyces in bioremediation of metal-polluted soils. CI - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Hellmann, Steffen AU - Hellmann S AD - Institute of Geosciences, Friedrich Schiller University Jena, Burgweg 11, 07749, Jena, Germany; International Max Planck Research School for Global Biogeochemical Cycles, Max Planck Institute for Biogeochemistry, Department of Biogeochemical Processes, Hans-Knoll-Strasse 10, 07745, Jena, Germany. FAU - Garcia-Cancela, Paula AU - Garcia-Cancela P AD - Department of Physical and Analytical Chemistry, Faculty of Chemistry, Julian Claveria 8, 33006, Oviedo, Spain; Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Av. Del Hospital Universitario s/n, 33011, Oviedo, Spain. FAU - Alonso-Fernandez, Sergio AU - Alonso-Fernandez S AD - Area de Microbiologia, Departamento de Biologia Funcional, IUOPA and ISPA, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain. FAU - Corte-Rodriguez, Mario AU - Corte-Rodriguez M AD - Department of Physical and Analytical Chemistry, Faculty of Chemistry, Julian Claveria 8, 33006, Oviedo, Spain; Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Av. Del Hospital Universitario s/n, 33011, Oviedo, Spain. FAU - Bettmer, Jorg AU - Bettmer J AD - Department of Physical and Analytical Chemistry, Faculty of Chemistry, Julian Claveria 8, 33006, Oviedo, Spain; Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Av. Del Hospital Universitario s/n, 33011, Oviedo, Spain. FAU - Manteca, Angel AU - Manteca A AD - Area de Microbiologia, Departamento de Biologia Funcional, IUOPA and ISPA, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain. FAU - Merten, Dirk AU - Merten D AD - Institute of Geosciences, Friedrich Schiller University Jena, Burgweg 11, 07749, Jena, Germany. FAU - Gil-Diaz, Teba AU - Gil-Diaz T AD - Institute of Applied Geosciences (AGW), Karlsruhe Institute of Technology (KIT), Adenauerring 20b, 76131, Karlsruhe, Germany. FAU - Schafer, Thorsten AU - Schafer T AD - Institute of Geosciences, Friedrich Schiller University Jena, Burgweg 11, 07749, Jena, Germany. Electronic address: thorsten.schaefer@uni-jena.de. FAU - Montes-Bayon, Maria AU - Montes-Bayon M AD - Department of Physical and Analytical Chemistry, Faculty of Chemistry, Julian Claveria 8, 33006, Oviedo, Spain; Instituto de Investigacion Sanitaria del Principado de Asturias (ISPA), Av. Del Hospital Universitario s/n, 33011, Oviedo, Spain. Electronic address: montesmaria@uniovi.es. LA - eng PT - Journal Article DEP - 20231109 PL - England TA - Chemosphere JT - Chemosphere JID - 0320657 RN - 00BH33GNGH (Cadmium) RN - 9G34HU7RV0 (Edetic Acid) RN - 0 (Metals) RN - 0 (Soil) SB - IM MH - *Streptomyces coelicolor MH - Cadmium/metabolism MH - Spores, Bacterial MH - Edetic Acid/metabolism MH - *Streptomyces/metabolism MH - Spores/metabolism MH - Metals/metabolism MH - Soil OTO - NOTNLM OT - Association OT - Bioaccumulation OT - Cadmium OT - Cerium OT - Gram-positive bacteria OT - Uranium COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Steffen Hellmann reports financial support was provided by German Research Foundation (DFG SPP, 2005), Opus Fluidum Futurum (project number 313773090, grant no. SCHA, 18547-1). Paula Garcia-Cancela reports financial support was provided by Government of Principado de Asturias (Severo Ochoa scholarship for predoctoral studies). Sergio Alonso-Fernandez reports financial support was provided by Consejeria de Ciencia, Innovacion y Universidad del Principado de Asturias (Severo Ochoa predoctoral grant (grant no. PA-20-PFBP19-006)). If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/11/12 00:41 MHDA- 2023/12/06 06:42 CRDT- 2023/11/11 19:16 PHST- 2023/08/30 00:00 [received] PHST- 2023/11/03 00:00 [revised] PHST- 2023/11/05 00:00 [accepted] PHST- 2023/12/06 06:42 [medline] PHST- 2023/11/12 00:41 [pubmed] PHST- 2023/11/11 19:16 [entrez] AID - S0045-6535(23)02903-X [pii] AID - 10.1016/j.chemosphere.2023.140633 [doi] PST - ppublish SO - Chemosphere. 2024 Jan;347:140633. doi: 10.1016/j.chemosphere.2023.140633. Epub 2023 Nov 9.