PMID- 37952497
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240207
IS  - 1873-4200 (Electronic)
IS  - 0301-4622 (Print)
IS  - 0301-4622 (Linking)
VI  - 304
DP  - 2024 Jan
TI  - Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural 
      inhibitors of amylin aggregation and cellular toxicity in pancreatic beta cells.
PG  - 107130
LID - S0301-4622(23)00181-3 [pii]
LID - 10.1016/j.bpc.2023.107130 [doi]
AB  - Impeding or reducing human amylin aggregation and/or its toxicity can be key to 
      preventing pancreatic islet amyloidosis and beta-cell loss in patients with Type 2 
      Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis 
      raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested 
      for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) 
      treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) 
      assay revealed an inhibitory trend of all three plant extracts against amylin 
      aggregates. In agreement with this finding, pomegranate peel and ironwort 
      extracts effectively prevented the transition of hIAPP from disordered, random 
      coil structures into aggregation prone beta-sheet enriched molecular assemblies, 
      revealed by CD spectroscopy. Consistent with their anti-aggregative action, all 
      three extracts prevented, to various degrees, reactive oxygen species (ROS) 
      accumulation, mitochondrial stress, and, ultimately, apoptosis of INS cells. 
      Collectively, the results from this study demonstrate effectiveness of natural 
      products to halt hIAPP aggregation, redox stress, and toxicity, which could be 
      exploited as novel therapeutics against amylin-derived islet amyloidosis and 
      beta-cell stress in T2DM.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Rishisree, Achanta
AU  - Rishisree A
AD  - Department of Biological Sciences, The George Washington University, Washington, 
      DC 20052, USA.
FAU - Mallory, Brayer
AU  - Mallory B
AD  - Department of Biological Sciences, The George Washington University, Washington, 
      DC 20052, USA.
FAU - Elena, Karnaukhova
AU  - Elena K
AD  - Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 
      Silver Spring, MD 20993, USA.
FAU - Teodora, Jankovic
AU  - Teodora J
AD  - Institute for Medicinal Plant Research "Dr. Josif Pancic", 11000 Belgrade, 
      Serbia.
FAU - Gordana, Zdunic
AU  - Gordana Z
AD  - Institute for Medicinal Plant Research "Dr. Josif Pancic", 11000 Belgrade, 
      Serbia.
FAU - Katarina, Savikin
AU  - Katarina S
AD  - Institute for Medicinal Plant Research "Dr. Josif Pancic", 11000 Belgrade, 
      Serbia.
FAU - Aleksandar, Jeremic
AU  - Aleksandar J
AD  - Department of Biological Sciences, The George Washington University, Washington, 
      DC 20052, USA. Electronic address: jerema@gwu.edu.
LA  - eng
GR  - R01 DK091845/DK/NIDDK NIH HHS/United States
GR  - R01 EB027648/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20231031
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Islet Amyloid Polypeptide)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - Islet Amyloid Polypeptide/chemistry
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - *Sideritis/metabolism
MH  - *Insulin-Secreting Cells
MH  - *Pomegranate/metabolism
MH  - *Amyloidosis/metabolism
MH  - Plant Extracts/pharmacology
PMC - PMC10841580
MID - NIHMS1944534
OTO - NOTNLM
OT  - Amylin
OT  - Mitochondrial stress
OT  - Plant extracts
OT  - Protein aggregation
OT  - Proteotoxicity
OT  - Reactive oxygen species
COIS- Declaration of Competing Interest Aleksandar Jeremic reports financial support 
      was provided by National Institute of Health.
EDAT- 2023/11/13 00:42
MHDA- 2023/12/17 09:44
PMCR- 2025/01/01
CRDT- 2023/11/12 18:12
PHST- 2023/07/01 00:00 [received]
PHST- 2023/10/27 00:00 [revised]
PHST- 2023/10/28 00:00 [accepted]
PHST- 2025/01/01 00:00 [pmc-release]
PHST- 2023/12/17 09:44 [medline]
PHST- 2023/11/13 00:42 [pubmed]
PHST- 2023/11/12 18:12 [entrez]
AID - S0301-4622(23)00181-3 [pii]
AID - 10.1016/j.bpc.2023.107130 [doi]
PST - ppublish
SO  - Biophys Chem. 2024 Jan;304:107130. doi: 10.1016/j.bpc.2023.107130. Epub 2023 Oct 
      31.