PMID- 37953747
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231114
IS  - 2666-3503 (Electronic)
IS  - 2666-3503 (Linking)
VI  - 4
DP  - 2023
TI  - The quality and quantity media-cultured mononuclear cell transplantation is safe 
      and effective in ischemic hindlimb mouse model.
PG  - 100129
LID - 10.1016/j.jvssci.2023.100129 [doi]
LID - 100129
AB  - OBJECTIVE: This study was conducted to investigate in vitro proangiogenic and 
      anti-inflammatory phenotypes and functions and the in vivo efficacy and safety of 
      quality and quantity (QQ) media-cultured mononuclear cells (MNCs) compared with 
      standard cultured MNCs from the peripheral blood of patients with chronic 
      limb-threatening ischemia (CLTI) with atherosclerotic risk factors. METHODS: 
      Peripheral blood MNCs (PBMNCs) from patients with CLTI were cultured in QQ 
      culture media or standard culture media. Phenotypic analysis of progenitor cells 
      (CD34(+)CD133(+)), M2 macrophages (CD206(+)), and inactivated T regulatory cells 
      (CD4(+)CD25(+)CD127(+)), colony-forming assay, and tube formation assay of QQ 
      media-cultured MNCs (QQMNCs) and PBMNCs, were conducted. Intramuscular 
      transplantation of QQMNCs or PBMNCs was performed in the ischemic hindlimb model. 
      The clinical appearance of ischemic limbs was observed, and blood flow in 
      ischemic limbs was measured using a laser Doppler perfusion imager. Outcomes were 
      compared between the QQMNC and PBMNC groups. RESULTS: Twenty patients with CLTI 
      were included. The mean percentages of CD34(+) cells, CD133(+) cells, 
      CD34(+)CD133(+) progenitor cells, CD206(+) cells, colony-forming cells, and tube 
      formation were significantly higher in the QQMNCs. The mean percentage of 
      CD4(+)CD25(+)CD127(+) cells was significantly lower in QQMNC. The colony-forming 
      unit count and Dil-acetylated low-density lipoprotein uptake were significantly 
      greater in QQMNCs. The clinical appearance of post-QQMNC-injected limbs was less 
      severe than the appearance of post-PBMNC-injected limbs. Limb perfusion was 
      significantly better in the QQMNCs. CONCLUSIONS: Proangiogenic and 
      anti-inflammatory phenotypes of MNCs cultured in QQ culture media were 
      reproducible. Intramuscular QQMNC transplantation was safe and resulted in better 
      reperfusion of ischemic hindlimbs compared with PBMNCs.
CI  - (c) 2023 by the Society for Vascular Surgery. Published by Elsevier Inc.
FAU - Chinchalongporn, Wanchai
AU  - Chinchalongporn W
AD  - Division of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok, Thailand.
FAU - Chruewkamlow, Nuttapol
AU  - Chruewkamlow N
AD  - Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, 
      Bangkok, Thailand.
FAU - Sermsathanasawadi, Nuttawut
AU  - Sermsathanasawadi N
AD  - Division of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok, Thailand.
FAU - Vorateera, Kosit
AU  - Vorateera K
AD  - Division of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok, Thailand.
FAU - Jintaworn, Suthatip
AU  - Jintaworn S
AD  - Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, 
      Bangkok, Thailand.
FAU - Wongwanit, Chumpol
AU  - Wongwanit C
AD  - Division of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok, Thailand.
FAU - Ruangsetakit, Chanean
AU  - Ruangsetakit C
AD  - Division of Vascular Surgery, Department of Surgery, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20230928
PL  - United States
TA  - JVS Vasc Sci
JT  - JVS-vascular science
JID - 101767073
PMC - PMC10632110
OTO - NOTNLM
OT  - Efficacy
OT  - Ischemic hindlimb mouse model
OT  - Quality and quantity media-cultured mononuclear cell transplantation
OT  - Safety
COIS- None.
EDAT- 2023/11/13 06:42
MHDA- 2023/11/13 06:43
PMCR- 2023/09/28
CRDT- 2023/11/13 04:15
PHST- 2023/08/02 00:00 [received]
PHST- 2023/09/17 00:00 [accepted]
PHST- 2023/11/13 06:43 [medline]
PHST- 2023/11/13 06:42 [pubmed]
PHST- 2023/11/13 04:15 [entrez]
PHST- 2023/09/28 00:00 [pmc-release]
AID - S2666-3503(23)00033-0 [pii]
AID - 100129 [pii]
AID - 10.1016/j.jvssci.2023.100129 [doi]
PST - epublish
SO  - JVS Vasc Sci. 2023 Sep 28;4:100129. doi: 10.1016/j.jvssci.2023.100129. 
      eCollection 2023.