PMID- 37957794
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240111
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 15
IP  - 6
DP  - 2023 Nov
TI  - Characterization of Hum j 6, a Major Allergen From Humulus japonicus Pollen, the 
      Primary Cause of Weed Pollinosis in East Asia.
PG  - 767-778
LID - 10.4168/aair.2023.15.6.767 [doi]
AB  - PURPOSE: Humulus japonicus (HJ) is one of the most important causes of weed 
      pollinosis in East Asia. The 10 kDa protein with pI 10 in 2-dimensional gel has 
      been recognized as the representative major allergen of HJ, but its major 
      allergens have not been characterized. This study aimed to characterize the major 
      allergen of HJ. METHODS: A major allergen in Japanese hop was detected by 
      proteome analysis; it was purified to homogeneity and its sequence was obtained 
      by transcriptome analysis. The recombinant proteins were produced in Escherichia 
      coli and Pichia expression systems, and their immunoglobulin E (IgE) reactivities 
      were compared to those of the natural counterpart. We also analyzed 
      post-translational modifications such as glycosylation and phosphorylation. 
      RESULTS: Pectin methylesterase inhibitor, Hum j 6, was found to be the major 
      allergen of HJ, and in silico signal peptide prediction corresponds to a 15.1 kDa 
      protein with a theoretical pI of 8.28. Natural Hum j 6 was recognized by IgE 
      antibodies from 86.4% (19/22) of HJ pollinosis patients, whereas the recombinant 
      proteins did not show strong IgE reactivity. No glycosylation was detected, while 
      at least 15 phosphorylated amino acids, possibly causing the pI and molecular 
      weight shift, were detected by tandem mass spectrometry analysis. CONCLUSIONS: 
      Hum j 6 was identified as the representative major allergen of HJ and seems to be 
      modified significantly after translation. These findings are useful for the 
      development of component-resolved diagnosis and immunotherapy.
CI  - Copyright (c) 2023 The Korean Academy of Asthma, Allergy and Clinical Immunology * 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AUID- ORCID: 0000-0001-9887-1426
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea. jeongky@yuhs.ac.
FAU - Sang, Minkyu
AU  - Sang M
AD  - Department of Biology, Soonchunhyang University, Asan, Korea.
FAU - Lee, Yong Seok
AU  - Lee YS
AD  - Department of Biology, Soonchunhyang University, Asan, Korea.
FAU - Gadermaier, Gabriele
AU  - Gadermaier G
AUID- ORCID: 0000-0002-4886-417X
AD  - Department of Bioscience and Medical Biology, Paris Lodron University Salzburg, 
      Salzburg, Austria.
FAU - Ferreira, Fatima
AU  - Ferreira F
AUID- ORCID: 0000-0003-0989-2335
AD  - Department of Bioscience and Medical Biology, Paris Lodron University Salzburg, 
      Salzburg, Austria.
FAU - Park, Jung-Won
AU  - Park JW
AUID- ORCID: 0000-0003-0249-8749
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea. parkjw@yuhs.ac.
LA  - eng
GR  - 2017R1D1A1B04028193/NRF/National Research Foundation of Korea/Korea
GR  - HR14C0006/Ministry of Health and Family Affairs/Korea
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC10643856
OTO - NOTNLM
OT  - Pollinosis
OT  - allergens
OT  - diagnosis
OT  - glycosylation
OT  - immunotherapy
OT  - phosphorylation
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2023/11/14 06:42
MHDA- 2023/11/14 06:43
PMCR- 2023/11/01
CRDT- 2023/11/14 00:19
PHST- 2023/03/16 00:00 [received]
PHST- 2023/05/30 00:00 [revised]
PHST- 2023/06/13 00:00 [accepted]
PHST- 2023/11/14 06:43 [medline]
PHST- 2023/11/14 06:42 [pubmed]
PHST- 2023/11/14 00:19 [entrez]
PHST- 2023/11/01 00:00 [pmc-release]
AID - 15.767 [pii]
AID - 10.4168/aair.2023.15.6.767 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2023 Nov;15(6):767-778. doi: 
      10.4168/aair.2023.15.6.767.