PMID- 37958932
OWN - NLM
STAT- MEDLINE
DCOM- 20231115
LR  - 20231117
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 21
DP  - 2023 Nov 3
TI  - Expression of Cannabinoid Receptors in the Trigeminal Ganglion of the Horse.
LID - 10.3390/ijms242115949 [doi]
LID - 15949
AB  - Cannabinoid receptors are expressed in human and animal trigeminal sensory 
      neurons; however, the expression in the equine trigeminal ganglion is unknown. 
      Ten trigeminal ganglia from five horses were collected post-mortem from an 
      abattoir. The expression of cannabinoid receptors type 1 (CB1R) and type 2 
      (CB2R), and the cannabinoid-related receptors like transient receptor potential 
      vanilloid type 1 (TRPV1), peroxisome proliferator-activated receptor gamma 
      (PPARɣ), and G protein-related receptor 55 (GPR55) in the trigeminal ganglia (TG) 
      of the horse were studied, using immunofluorescence on cryosections and 
      formalin-fixed paraffin-embedded (FFPE) sections. Neurons and glial cells were 
      identified using fluorescent Nissl staining NeuroTrace((R)) and an antibody 
      directed against the glial marker glial fibrillary acidic protein (GFAP), 
      respectively. Macrophages were identified by means of an antibody directed 
      against the macrophages/microglia marker ionized calcium-binding adapter molecule 
      1 (IBA1). The protein expression of CB1R, CB2R, TRPV1, and PPARɣ was found in the 
      majority of TG neurons in both cryosections and FFPE sections. The expression of 
      GPR55 immunoreactivity was mainly detectable in FFPE sections, with expression in 
      the majority of sensory neurons. Some receptors were also observed in glial cells 
      (CB2R, TRPV1, PPARgamma, and GPR55) and inflammatory cells (PPARgamma and GPR55). These 
      results support further investigation of such receptors in disorders of equine 
      trigeminal neuronal excitability.
FAU - Zamith Cunha, Rodrigo
AU  - Zamith Cunha R
AUID- ORCID: 0000-0002-8548-6905
AD  - Department of Veterinary Medical Sciences, University of Bologna, 37200 Bologna, 
      Italy.
FAU - Semprini, Alberto
AU  - Semprini A
AD  - Department of Veterinary Medical Sciences, University of Bologna, 37200 Bologna, 
      Italy.
FAU - Salamanca, Giulia
AU  - Salamanca G
AUID- ORCID: 0000-0003-4322-0030
AD  - Department of Veterinary Medical Sciences, University of Bologna, 37200 Bologna, 
      Italy.
FAU - Gobbo, Francesca
AU  - Gobbo F
AUID- ORCID: 0000-0002-4082-4675
AD  - Department of Veterinary Medical Sciences, University of Bologna, 37200 Bologna, 
      Italy.
FAU - Morini, Maria
AU  - Morini M
AUID- ORCID: 0000-0002-5803-4487
AD  - Department of Veterinary Medical Sciences, University of Bologna, 37200 Bologna, 
      Italy.
FAU - Pickles, Kirstie J
AU  - Pickles KJ
AD  - School of Veterinary Medicine and Science, University of Nottingham, Nottingham 
      LE12 5RD, UK.
FAU - Roberts, Veronica
AU  - Roberts V
AD  - Bristol Vet School, University of Bristol, Bristol BS40 5DU, UK.
FAU - Chiocchetti, Roberto
AU  - Chiocchetti R
AUID- ORCID: 0000-0003-4448-6199
AD  - Department of Veterinary Medical Sciences, University of Bologna, 37200 Bologna, 
      Italy.
LA  - eng
GR  - 2023/Formula Swiss AG, Swiss/
PT  - Journal Article
DEP - 20231103
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Receptors, Cannabinoid)
RN  - 0 (PPAR gamma)
RN  - 0 (TRPV Cation Channels)
SB  - IM
MH  - Humans
MH  - Horses
MH  - Animals
MH  - Receptors, Cannabinoid/metabolism
MH  - *Trigeminal Ganglion/metabolism
MH  - *PPAR gamma/metabolism
MH  - Neurons/metabolism
MH  - Neuroglia/metabolism
MH  - TRPV Cation Channels/genetics/metabolism
PMC - PMC10648827
OTO - NOTNLM
OT  - G protein-related receptor 55
OT  - cannabidiol
OT  - equine
OT  - immunofluorescence
OT  - peroxisome proliferator-activated receptor gamma
OT  - transient receptor potential vanilloid type 1
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/14 06:42
MHDA- 2023/11/15 06:42
PMCR- 2023/11/03
CRDT- 2023/11/14 02:10
PHST- 2023/09/10 00:00 [received]
PHST- 2023/10/17 00:00 [revised]
PHST- 2023/10/31 00:00 [accepted]
PHST- 2023/11/15 06:42 [medline]
PHST- 2023/11/14 06:42 [pubmed]
PHST- 2023/11/14 02:10 [entrez]
PHST- 2023/11/03 00:00 [pmc-release]
AID - ijms242115949 [pii]
AID - ijms-24-15949 [pii]
AID - 10.3390/ijms242115949 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 3;24(21):15949. doi: 10.3390/ijms242115949.