PMID- 37958960
OWN - NLM
STAT- MEDLINE
DCOM- 20231115
LR  - 20231117
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 21
DP  - 2023 Nov 5
TI  - Therapeutic Nanodiamonds Containing Icariin Ameliorate the Progression of 
      Osteoarthritis in Rats.
LID - 10.3390/ijms242115977 [doi]
LID - 15977
AB  - In present study, icariin (ICA)/tannic acid (TA)-nanodiamonds (NDs) were prepared 
      as follows. ICA was anchored to ND surfaces with absorbed TA (ICA/TA-NDs) and we 
      evaluated their in vitro anti-inflammatory effects on lipopolysaccharide 
      (LPS)-activated macrophages and in vivo cartilage protective effects on a rat 
      model of monosodium iodoacetate (MIA)-induced osteoarthritis (OA). The ICA/TA-NDs 
      showed prolonged release of ICA from the NDs for up to 28 days in a sustained 
      manner. ICA/TA-NDs inhibited the mRNA levels of pro-inflammatory elements, 
      including matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), 
      interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), and increased the mRNA 
      levels of anti-inflammatory factors (i.e., IL-4 and IL-10) in LPS-activated RAW 
      264.7 macrophages. Animal studies exhibited that intra-articular injection of 
      ICA/TA-NDs notably suppressed levels of IL-6, MMP-3, and TNF-alpha and induced level 
      of IL-10 in serum of MIA-induced OA rat models in a dose-dependent manner. 
      Furthermore, these noticeable anti-inflammatory effects of ICA/TA-NDs remarkably 
      contributed to the protection of the progression of MIA-induced OA and cartilage 
      degradation, as exhibited by micro-computed tomography (micro-CT), gross 
      findings, and histological investigations. Accordingly, in vitro and in vivo 
      findings suggest that the prolonged ICA delivery of ICA/TA-NDs possesses an 
      excellent latent to improve inflammation as well as defend against cartilage 
      disorder in OA.
FAU - Yu, Ying
AU  - Yu Y
AD  - Department of Orthopedic Surgery and Nano-Based Disease Control Institute, Korea 
      University Guro Hospital, Seoul 08308, Republic of Korea.
FAU - Kim, Sang-Min
AU  - Kim SM
AD  - Department of Orthopedic Surgery and Nano-Based Disease Control Institute, Korea 
      University Guro Hospital, Seoul 08308, Republic of Korea.
FAU - Park, Kyeongsoon
AU  - Park K
AUID- ORCID: 0000-0003-3625-4128
AD  - Department of Systems Biotechnology, Chung-Ang University, Anseong 17546, 
      Republic of Korea.
FAU - Kim, Hak Jun
AU  - Kim HJ
AD  - Department of Orthopedic Surgery and Nano-Based Disease Control Institute, Korea 
      University Guro Hospital, Seoul 08308, Republic of Korea.
FAU - Kim, Jae Gyoon
AU  - Kim JG
AD  - Department of Orthopedic Surgery, Korea University Ansan Hospital, Korea 
      University College of Medicine, Ansansi 15355, Republic of Korea.
FAU - Kim, Sung Eun
AU  - Kim SE
AUID- ORCID: 0000-0001-9445-2451
AD  - Department of Orthopedic Surgery and Nano-Based Disease Control Institute, Korea 
      University Guro Hospital, Seoul 08308, Republic of Korea.
LA  - eng
GR  - K2023121/Korea University Grants/
PT  - Journal Article
DEP - 20231105
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - VNM47R2QSQ (icariin)
RN  - 130068-27-8 (Interleukin-10)
RN  - 0 (Nanodiamonds)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Anti-Inflammatory Agents)
RN  - WF5188V710 (Iodoacetic Acid)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Interleukin-10/metabolism
MH  - *Nanodiamonds
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - X-Ray Microtomography
MH  - *Cartilage, Articular/metabolism
MH  - *Osteoarthritis/metabolism
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Iodoacetic Acid/adverse effects
MH  - RNA, Messenger/metabolism
MH  - Disease Models, Animal
PMC - PMC10647515
OTO - NOTNLM
OT  - cartilage degradation
OT  - icariin
OT  - inflammation
OT  - nanodiamonds
OT  - osteoarthritis
OT  - tannic acid
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/14 06:43
MHDA- 2023/11/15 06:43
PMCR- 2023/11/05
CRDT- 2023/11/14 02:11
PHST- 2023/10/04 00:00 [received]
PHST- 2023/10/29 00:00 [revised]
PHST- 2023/11/02 00:00 [accepted]
PHST- 2023/11/15 06:43 [medline]
PHST- 2023/11/14 06:43 [pubmed]
PHST- 2023/11/14 02:11 [entrez]
PHST- 2023/11/05 00:00 [pmc-release]
AID - ijms242115977 [pii]
AID - ijms-24-15977 [pii]
AID - 10.3390/ijms242115977 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 5;24(21):15977. doi: 10.3390/ijms242115977.