PMID- 37958975
OWN - NLM
STAT- MEDLINE
DCOM- 20231115
LR  - 20231122
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 21
DP  - 2023 Nov 6
TI  - Study on the Treatment of ITP Mice with IVIG Sourced from Distinct Sex-Special 
      Plasma (DSP-IVIG).
LID - 10.3390/ijms242115993 [doi]
LID - 15993
AB  - Intravenous immunoglobulin (IVIG) is a first-line drug prepared from human plasma 
      for the treatment of autoimmune diseases (AIDs), especially immune 
      thrombocytopenia (ITP). Significant differences exist in protein types and 
      expression levels between male and female plasma, and the prevalence of 
      autoimmune diseases varies between sexes. The present study seeks to explore 
      potential variations in IVIG sourced from distinct sex-specific plasma 
      (DSP-IVIG), including IVIG sourced from female plasma (F-IVIG), IVIG sourced from 
      male plasma (M-IVIG), and IVIG sourced from a blend of male and female plasma 
      (Mix-IVIG). To address this question, we used an ITP mouse model and a 
      monocyte-macrophage inflammation model treated with DSP IVIG. The analysis of 
      proteomics in mice suggested that the pathogenesis and treatment of ITP may 
      involve FcgammaRs mediated phagocytosis, apoptosis, Th17, cytokines, chemokines, and 
      more. Key indicators, including the mouse spleen index, CD16(+) macrophages, M1, 
      M2, IL-6, IL-27, and IL-13, all indicated that the efficacy in improving ITP was 
      highest for M-IVIG. Subsequent cell experiments revealed that M-IVIG exhibited a 
      more potent ability to inhibit monocyte phagocytosis. It induced more necrotic M2 
      cells and fewer viable M2, resulting in weaker M2 phagocytosis. M-IVIG also 
      demonstrated superiority in the downregulation of surface makers CD36, CD68, and 
      CD16 on M1 macrophages, a weaker capacity to activate complement, and a stronger 
      binding ability to FcgammaRs on the THP-1 surface. In summary, DSP-IVIG effectively 
      mitigated inflammation in ITP mice and monocytes and macrophages. However, M-IVIG 
      exhibited advantages in improving the spleen index, regulating the number and 
      typing of M1 and M2 macrophages, and inhibiting macrophage-mediated inflammation 
      compared to F-IVIG and Mix-IVIG.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Yuan, Xin
AU  - Yuan X
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Wang, Zongkui
AU  - Wang Z
AUID- ORCID: 0000-0002-5764-7660
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Xu, Jixuan
AU  - Xu J
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Ye, Shengliang
AU  - Ye S
AUID- ORCID: 0000-0003-4477-3075
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Jiang, Peng
AU  - Jiang P
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Du, Xi
AU  - Du X
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Liu, Fengjuan
AU  - Liu F
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Lin, Fangzhao
AU  - Lin F
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Zhang, Rong
AU  - Zhang R
AUID- ORCID: 0000-0003-4480-7191
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Ma, Li
AU  - Ma L
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
FAU - Li, Changqing
AU  - Li C
AD  - Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Chengdu 610052, China.
LA  - eng
GR  - 2023NSFSC0714, 2023ZHCG0066 and 2022YFS0010/Department of Science and Technology 
      of Sichuan Province/
GR  - CIFMS, 2021-I2M-1-042/CAMS Innovation Fund for Medical Sciences/
GR  - Q22009/Scientific Research Project of Sichuan Medical Association/
PT  - Journal Article
DEP - 20231106
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Cytokines)
SB  - IM
MH  - Male
MH  - Female
MH  - Humans
MH  - Animals
MH  - Mice
MH  - Immunoglobulins, Intravenous/therapeutic use
MH  - *Purpura, Thrombocytopenic, Idiopathic/drug therapy
MH  - *Thrombocytopenia/drug therapy
MH  - Cytokines
MH  - Inflammation/drug therapy
PMC - PMC10648144
OTO - NOTNLM
OT  - IVIG sourced from distinct sex-specific plasma (DSP-IVIG)
OT  - immune thrombocytopenia
OT  - intravenous immunoglobulin
OT  - macrophages
OT  - proteomics
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/14 06:43
MHDA- 2023/11/15 06:43
PMCR- 2023/11/06
CRDT- 2023/11/14 02:11
PHST- 2023/10/07 00:00 [received]
PHST- 2023/10/30 00:00 [revised]
PHST- 2023/11/03 00:00 [accepted]
PHST- 2023/11/15 06:43 [medline]
PHST- 2023/11/14 06:43 [pubmed]
PHST- 2023/11/14 02:11 [entrez]
PHST- 2023/11/06 00:00 [pmc-release]
AID - ijms242115993 [pii]
AID - ijms-24-15993 [pii]
AID - 10.3390/ijms242115993 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Nov 6;24(21):15993. doi: 10.3390/ijms242115993.