PMID- 37960061
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231117
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 12
IP  - 21
DP  - 2023 Oct 27
TI  - Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species 
      Using Oligo-FISH.
LID - 10.3390/plants12213705 [doi]
LID - 3705
AB  - The genome composition of intermediate wheatgrass (IWG) is complex and continues 
      to be a subject of investigation. In this study, molecular cytogenetics were used 
      to investigate the karyotype composition of Th. intermedium and its relative 
      diploid species. St(2)-80 developed from Pseudowroegneria strigose and pDb12H 
      developed from Dasypyrum breviaristatum were used as probes in fluorescence in 
      situ hybridization (FISH) to classify the chromosomes of Th. intermedium into 
      three groups, expressed as J(vs)J(vs)J(r)J(r)StSt. A combined multiplex 
      oligonucleotide probe, including pSc119.2-1, (GAA)(10), AFA-3, AFA-4, pAs1-1, 
      Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten 
      accessions of Th. intermedium. Variability among and within the studied 
      accessions of intermediate wheatgrass was observed in their FISH patterns. 
      Results of this study led to the conclusions that J(vs) had largely been 
      contributed from Da. breviaristatum, but not the present-day Da. villosum; IWG 
      had only one J genome, J(r), which was related to either Th. elongatum or Th. 
      bessarabicum; and St was contributed from the genus Pseudoroegneria by 
      hybridization with Th. junceiforme or Th. sartorii.
FAU - Qi, Fei
AU  - Qi F
AD  - State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 
      271018, China.
FAU - Liang, Shuang
AU  - Liang S
AD  - Agronomy College, Shandong Agricultural University, Tai'an 271018, China.
FAU - Xing, Piyi
AU  - Xing P
AD  - State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 
      271018, China.
AD  - Agronomy College, Shandong Agricultural University, Tai'an 271018, China.
FAU - Bao, Yinguang
AU  - Bao Y
AD  - State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 
      271018, China.
AD  - Agronomy College, Shandong Agricultural University, Tai'an 271018, China.
FAU - Wang, Richard R-C
AU  - Wang RR
AUID- ORCID: 0000-0003-3622-2572
AD  - USDA-ARS Forage & Range Research Laboratory (FRRL), Logan, UT 84322-6300, USA.
FAU - Li, Xingfeng
AU  - Li X
AD  - State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 
      271018, China.
AD  - Agronomy College, Shandong Agricultural University, Tai'an 271018, China.
LA  - eng
GR  - CRIS 2080-21000-018-000D/USDA-ARS/
PT  - Journal Article
DEP - 20231027
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC10650893
OTO - NOTNLM
OT  - FISH
OT  - Triticeae
OT  - evolution
OT  - genome constitution
OT  - karyotype
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/14 06:43
MHDA- 2023/11/14 06:44
PMCR- 2023/10/27
CRDT- 2023/11/14 02:17
PHST- 2023/09/22 00:00 [received]
PHST- 2023/10/13 00:00 [revised]
PHST- 2023/10/20 00:00 [accepted]
PHST- 2023/11/14 06:44 [medline]
PHST- 2023/11/14 06:43 [pubmed]
PHST- 2023/11/14 02:17 [entrez]
PHST- 2023/10/27 00:00 [pmc-release]
AID - plants12213705 [pii]
AID - plants-12-03705 [pii]
AID - 10.3390/plants12213705 [doi]
PST - epublish
SO  - Plants (Basel). 2023 Oct 27;12(21):3705. doi: 10.3390/plants12213705.