PMID- 37960348
OWN - NLM
STAT- MEDLINE
DCOM- 20231115
LR  - 20231117
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 15
IP  - 21
DP  - 2023 Nov 6
TI  - Effects of a High-Fat Diet and Docosahexaenoic Acid during Pregnancy on Fatty 
      Acid Composition in the Fetal Livers of Mice.
LID - 10.3390/nu15214696 [doi]
LID - 4696
AB  - A high-fat diet (HFD) during pregnancy promotes fat accumulation and reduces 
      docosahexaenoic acid (DHA) levels in the liver of the offspring at postnatal 
      ages, which can depend on fetal sex. However, the prenatal mechanisms behind 
      these associations are still unclear. Thus, we analyzed if an HFD alters DHA 
      content and the expression of molecules related to fatty acid (FA) metabolism in 
      the fetal liver. Female C57BL/6 mice were fed a control diet or HFD for 4-6 weeks 
      before pregnancy until the gestational day (GD) 17.5. A subgroup of each diet 
      received DHA (100 mg/Kg) orally from GD 6.5 until 16.5. On GD 17.5, maternal 
      livers, placentas, and livers from male and female fetuses were collected for FA 
      profiling with gas-chromatography and gene expression of molecules related to FA 
      metabolism using qPCR. PPAR-alpha protein expression was evaluated using Western 
      blot. The gene expression of placental FA transporters was also assessed. An HFD 
      increased eicosapentaenoic acid (EPA) and decreased DHA levels and protein 
      expression of PPAR-alpha in the fetal livers of both sexes. DHA increased the gene 
      expression of Ppara, Cpt1, and Acsl1 in the livers of female fetuses. Therefore, 
      an HFD reduces DHA levels and PPAR-alpha, a master regulator of gene expression, in 
      the fetal liver. In turn, the livers of female fetuses seem to be more sensitive 
      to DHA action.
FAU - Alvarez, Daniela
AU  - Alvarez D
AD  - Laboratory of Endocrinology and Metabolism, Department of Internal Medicine West 
      Division, Universidad de Chile, Santiago 8350499, Chile.
FAU - Ortiz, Macarena
AU  - Ortiz M
AD  - Laboratory of Endocrinology and Metabolism, Department of Internal Medicine West 
      Division, Universidad de Chile, Santiago 8350499, Chile.
FAU - Valdebenito, Gabriel
AU  - Valdebenito G
AUID- ORCID: 0000-0002-7169-6653
AD  - Laboratory of Endocrinology and Metabolism, Department of Internal Medicine West 
      Division, Universidad de Chile, Santiago 8350499, Chile.
FAU - Crisosto, Nicolas
AU  - Crisosto N
AD  - Laboratory of Endocrinology and Metabolism, Department of Internal Medicine West 
      Division, Universidad de Chile, Santiago 8350499, Chile.
AD  - Endocrinology Unit, Department of Medicine, Clinica Alemana de Santiago, Faculty 
      of Medicine, Universidad del Desarrollo, Santiago 7610658, Chile.
FAU - Echiburu, Barbara
AU  - Echiburu B
AD  - Laboratory of Endocrinology and Metabolism, Department of Internal Medicine West 
      Division, Universidad de Chile, Santiago 8350499, Chile.
FAU - Valenzuela, Rodrigo
AU  - Valenzuela R
AUID- ORCID: 0000-0001-9298-6142
AD  - Nutrition Department, School of Medicine, Universidad de Chile, Santiago 8380000, 
      Chile.
FAU - Espinosa, Alejandra
AU  - Espinosa A
AUID- ORCID: 0000-0003-4779-5341
AD  - Department of Medical Technology, Faculty of Medicine, University of Chile, 
      Santiago 8380453, Chile.
FAU - Maliqueo, Manuel
AU  - Maliqueo M
AUID- ORCID: 0000-0003-3872-0217
AD  - Laboratory of Endocrinology and Metabolism, Department of Internal Medicine West 
      Division, Universidad de Chile, Santiago 8350499, Chile.
LA  - eng
GR  - FONDECYT 1181798/Agencia Nacional de Investigacion y Desarrollo/
GR  - FONDECYT 1181774/Agencia Nacional de Investigacion y Desarrollo/
GR  - FONDECYT 1201483/Agencia Nacional de Investigacion y Desarrollo/
GR  - FONDECYT 1221098/Agencia Nacional de Investigacion y Desarrollo/
GR  - CONICYT-PFCHA/Doctorado Nacional/2018-21181253/Agencia Nacional de Investigacion 
      y Desarrollo/
PT  - Journal Article
DEP - 20231106
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Fatty Acids)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - 0 (Peroxisome Proliferator-Activated Receptors)
SB  - IM
MH  - Mice
MH  - Female
MH  - Pregnancy
MH  - Male
MH  - Animals
MH  - *Fatty Acids/metabolism
MH  - *Diet, High-Fat/adverse effects
MH  - Docosahexaenoic Acids/pharmacology
MH  - Placenta/metabolism
MH  - Peroxisome Proliferator-Activated Receptors/metabolism
MH  - Mice, Inbred C57BL
MH  - Liver/metabolism
PMC - PMC10649644
OTO - NOTNLM
OT  - docosahexaenoic acid
OT  - fetal liver
OT  - high-fat diet
OT  - maternal liver
OT  - placenta
OT  - pregnancy
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/14 06:43
MHDA- 2023/11/15 06:43
PMCR- 2023/11/06
CRDT- 2023/11/14 02:19
PHST- 2023/10/04 00:00 [received]
PHST- 2023/10/27 00:00 [revised]
PHST- 2023/11/03 00:00 [accepted]
PHST- 2023/11/15 06:43 [medline]
PHST- 2023/11/14 06:43 [pubmed]
PHST- 2023/11/14 02:19 [entrez]
PHST- 2023/11/06 00:00 [pmc-release]
AID - nu15214696 [pii]
AID - nutrients-15-04696 [pii]
AID - 10.3390/nu15214696 [doi]
PST - epublish
SO  - Nutrients. 2023 Nov 6;15(21):4696. doi: 10.3390/nu15214696.