PMID- 37966680
OWN - NLM
STAT- MEDLINE
DCOM- 20240101
LR  - 20240106
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 49
IP  - 6
DP  - 2023 Dec
TI  - Kisspeptin modulation of nonapeptide and cytochrome P450 aromatase mRNA 
      expression in the brain and ovary of the catfish Heteropneustes fossilis: in vivo 
      and in vitro studies.
PG  - 1489-1509
LID - 10.1007/s10695-023-01270-w [doi]
AB  - In Heteropneustes fossilis, kisspeptins (Kiss) and nonapeptides (NPs; vasotocin, 
      Vt; isotocin, Itb; Val8-isotocin, Ita) stimulate the 
      hypothalamus-pituitary-gonadal (HPG) axis, and estrogen feedback modulates the 
      expression of these systems. In this study, functional interactions among these 
      regulatory systems were demonstrated in the brain and ovary at the mRNA 
      expression level. Human KISS1 (hKISS1) and H. fossilis Kiss2 (HfKiss2) produced 
      biphasic effects on brain and ovarian vt, itb and ita expression at 24 h post 
      injection: low and median doses produced inhibition, no change or mild 
      stimulation, and the highest dose consistently stimulated the mRNA levels. The 
      Kiss peptides produced an upregulation of NP mRNA expression at 24 h incubation 
      of brain and ovarian slices by increasing the concentration of hKISS1 and 
      HfKiss2. The kiss peptides stimulated brain cyp19a1b and ovary cyp19a1a 
      expression, both in vivo and in vitro. Peptide234, a Kiss1 receptor antagonist, 
      inhibited basal mRNA expression of the NPs, cyp19a1b and cyp19a1a, which was 
      prevented by the Kiss peptides, both in vivo and in vitro. In all the 
      experiments, HfKiss2 was more effective than hKISS1 in modulating mRNA 
      expression. The results suggest that the NP and E(2) systems are functional 
      targets of Kiss peptides and interact with each other.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Chaube, Radha
AU  - Chaube R
AD  - Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 
      221005, India.
FAU - Sharma, Sandhya
AU  - Sharma S
AD  - Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 
      221005, India.
FAU - Joy, Keerikkattil
AU  - Joy K
AD  - , Ernakulam, India.
LA  - eng
PT  - Journal Article
DEP - 20231115
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - 0 (Kisspeptins)
RN  - EC 1.14.14.1 (Aromatase)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Female
MH  - Humans
MH  - Animals
MH  - *Ovary/metabolism
MH  - Kisspeptins/genetics/pharmacology/metabolism
MH  - *Catfishes/metabolism
MH  - Aromatase/genetics/metabolism
MH  - Brain/metabolism
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - Catfish brain and ovary
OT  - Nonapeptide gene expression
OT  - P450 aromatase expression
OT  - Synthetic Kiss peptides
EDAT- 2023/11/15 12:43
MHDA- 2024/01/02 11:46
CRDT- 2023/11/15 11:12
PHST- 2022/09/19 00:00 [received]
PHST- 2023/11/09 00:00 [accepted]
PHST- 2024/01/02 11:46 [medline]
PHST- 2023/11/15 12:43 [pubmed]
PHST- 2023/11/15 11:12 [entrez]
AID - 10.1007/s10695-023-01270-w [pii]
AID - 10.1007/s10695-023-01270-w [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2023 Dec;49(6):1489-1509. doi: 10.1007/s10695-023-01270-w. 
      Epub 2023 Nov 15.