PMID- 37967592
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240405
IS  - 1873-6815 (Electronic)
IS  - 0531-5565 (Linking)
VI  - 184
DP  - 2023 Dec
TI  - MiR-20b-5p involves in vascular aging induced by hyperhomocysteinemia.
PG  - 112330
LID - S0531-5565(23)00251-6 [pii]
LID - 10.1016/j.exger.2023.112330 [doi]
AB  - Hyperhomocysteinemia (HHcy) is an independent risk factor of atherosclerosis 
      (AS). Some reports have shown that homocysteine (Hcy) could accelerate the 
      development of AS by promoting endothelial cell senescence. miRNAs were widely 
      involved in the pathophysiology of HHcy. However, few studies have focused on the 
      changes of miRNA-mRNA networks in the artery of HHcy patients. For this reason, 
      RNA-sequencing was adopted to investigate the expression of miRNA and mRNA in 
      HHcy model mouse arteries. We found that the expression of 216 mRNAs and 48 
      miRNAs were significantly changed. Using TargetScan and miRDB web tools, 29 
      miRNA-mRNA pairs were predicted. Notably, miR-20b-5p and FJX1 shared the highest 
      predicted score in TargetScan, and further study indicated that the miR-20b-5p 
      inhibitor significantly upregulated the FJX1 expression in HHcy human umbilical 
      vein endothelial cells (HUVECs) model. PPI analysis revealed an important 
      sub-network which was centered on CDK1. Gene ontology (GO) enrichment analysis 
      showed that HHcy had a significant effect on cell cycle. Further experiments 
      found that Hcy management increased reactive oxygen species (ROS) generation, the 
      activity of senescence associated beta-galactosidase (SA-beta-gal) and the protein 
      expression of p16 and p21 in HUVECs, which were rescued by miR-20b-5p inhibitor. 
      In general, our research indicated the important role of miR-20b-5p in 
      HHcy-related endothelial cell senescence.
CI  - Copyright (c) 2023. Published by Elsevier Inc.
FAU - Qin, Hao
AU  - Qin H
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Hu, Long-Long
AU  - Hu LL
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Wang, Wen-Jun
AU  - Wang WJ
AD  - Department of Respiratory Diseases, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Yu, Zuo-Zhong
AU  - Yu ZZ
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Chen, Yang
AU  - Chen Y
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Zhao, Yuan-Bin
AU  - Zhao YB
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Liao, Yan-Hui
AU  - Liao YH
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Zhang, Wei-Lin
AU  - Zhang WL
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China.
FAU - Yang, Ren-Qiang
AU  - Yang RQ
AD  - Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang 
      University, Nanchang 330006, Jiangxi, People's Republic of China. Electronic 
      address: Yang.RQ@ncu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231116
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Atherosclerosis/genetics
MH  - Cellular Senescence/genetics
MH  - Human Umbilical Vein Endothelial Cells
MH  - *Hyperhomocysteinemia/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - Atherosclerosis (AS)
OT  - FJX1
OT  - Hyperhomocysteinemia (HHcy)
OT  - Senescence
OT  - miR-20b-5p
COIS- Declaration of competing interest The authors declare no conflicts of interest 
      regarding this work. The corresponding authors have the right to and do speak on 
      behalf of all authors.
EDAT- 2023/11/16 00:42
MHDA- 2023/12/17 13:19
CRDT- 2023/11/15 19:20
PHST- 2023/10/18 00:00 [received]
PHST- 2023/11/09 00:00 [revised]
PHST- 2023/11/12 00:00 [accepted]
PHST- 2023/12/17 13:19 [medline]
PHST- 2023/11/16 00:42 [pubmed]
PHST- 2023/11/15 19:20 [entrez]
AID - S0531-5565(23)00251-6 [pii]
AID - 10.1016/j.exger.2023.112330 [doi]
PST - ppublish
SO  - Exp Gerontol. 2023 Dec;184:112330. doi: 10.1016/j.exger.2023.112330. Epub 2023 
      Nov 16.