PMID- 37967727
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231229
IS  - 1872-8006 (Electronic)
IS  - 0304-4165 (Linking)
VI  - 1868
IP  - 1
DP  - 2024 Jan
TI  - High-fat high-fructose diet and alpha-ketoglutarate affect mouse behavior that is 
      accompanied by changes in oxidative stress response and energy metabolism in the 
      cerebral cortex.
PG  - 130521
LID - S0304-4165(23)00219-2 [pii]
LID - 10.1016/j.bbagen.2023.130521 [doi]
AB  - BACKGROUND: High caloric diets with high amounts of fats and sweeteners such as 
      fructose may predispose organisms to neurodegenerative diseases. METHODS: This 
      study aimed to examine the effects of a high-fat high-fructose diet (HFFD) on the 
      behavior of mice, energy metabolism, and markers of oxidative stress in murine 
      cerebral cortex. Dietary alpha-ketoglutarate (AKG) was chosen as a treatment which 
      could modulate the putative effects of HFFD. RESULTS: We found that HFFD 
      stimulated locomotion and defecation in mice, whereas an AKG-supplemented diet 
      had a proclivity to promote anxiety-like behavior. HFFD stimulated lipid 
      peroxidation, and in turn, the AKG-supplemented diet led to a higher ratio of 
      reduced to oxidized glutathione, higher activity of NAD(P)H:quinone 
      oxidoreductase 1, and higher mRNA levels of UDP-glucose 6-dehydrogenase and 
      transcription factor EB. Both diets separately, but not in combination, led to a 
      decrease in the activities of glutathione peroxidase, glutathione S-transferase, 
      and phosphofructokinase. All experimental diets resulted in lower levels of 
      transcripts of genes encoding pyruvate dehydrogenase kinase 4 (PDK4), glycine 
      N-methyl transferase, and peroxisome proliferator receptor gamma co-activator 1. 
      CONCLUSIONS: Our results show that diet supplemented with AKG resulted in effects 
      similar to those of HFFD on the cerebral cortex, but elicited substantial 
      differences between these two diets with respect to behavior, 
      glutathione-dependent detoxification, and processes related to autophagy. GENERAL 
      SIGNIFICANCE: Our study provides insight into the metabolic effects of HFFD alone 
      and in combination with alpha-ketoglutarate in the mouse brain.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Demianchuk, Oleh
AU  - Demianchuk O
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Vatashchuk, Myroslava
AU  - Vatashchuk M
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Gospodaryov, Dmytro
AU  - Gospodaryov D
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine. 
      Electronic address: dmytro.gospodaryov@pnu.edu.ua.
FAU - Hurza, Viktoria
AU  - Hurza V
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Ivanochko, Marian
AU  - Ivanochko M
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Derkachov, Vitalii
AU  - Derkachov V
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Berezovskyi, Vladyslav
AU  - Berezovskyi V
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine.
FAU - Lushchak, Oleh
AU  - Lushchak O
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine; 
      Research and Development University, 13a Shota Rustaveli Str., Ivano-Frankivsk, 
      76018, Ukraine.
FAU - Storey, Kenneth B
AU  - Storey KB
AD  - Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, 
      Ontario K1S 5B6, Canada.
FAU - Bayliak, Maria
AU  - Bayliak M
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine. 
      Electronic address: maria.bayliak@pnu.edu.ua.
FAU - Lushchak, Volodymyr I
AU  - Lushchak VI
AD  - Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian 
      National University, 57 Shevchenko Str., Ivano-Frankivsk, 76018, Ukraine; 
      Research and Development University, 13a Shota Rustaveli Str., Ivano-Frankivsk, 
      76018, Ukraine. Electronic address: volodymyr.lushchak@pnu.edu.ua.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231113
PL  - Netherlands
TA  - Biochim Biophys Acta Gen Subj
JT  - Biochimica et biophysica acta. General subjects
JID - 101731726
RN  - 0 (Ketoglutaric Acids)
RN  - 30237-26-4 (Fructose)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Ketoglutaric Acids
MH  - *Fructose
MH  - Diet, High-Fat/adverse effects
MH  - Oxidative Stress
MH  - Energy Metabolism
OTO - NOTNLM
OT  - Behavior
OT  - Brain
OT  - Glycolysis
OT  - High caloric diet
OT  - Mitochondria, oxidative stress
OT  - alpha-Ketoglutarate
COIS- Declaration of Competing Interest The authors declare that they have no conflict 
      of interest.
EDAT- 2023/11/16 00:42
MHDA- 2023/12/17 09:43
CRDT- 2023/11/15 19:23
PHST- 2023/08/28 00:00 [received]
PHST- 2023/10/19 00:00 [revised]
PHST- 2023/11/09 00:00 [accepted]
PHST- 2023/12/17 09:43 [medline]
PHST- 2023/11/16 00:42 [pubmed]
PHST- 2023/11/15 19:23 [entrez]
AID - S0304-4165(23)00219-2 [pii]
AID - 10.1016/j.bbagen.2023.130521 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Gen Subj. 2024 Jan;1868(1):130521. doi: 
      10.1016/j.bbagen.2023.130521. Epub 2023 Nov 13.