PMID- 37967788
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231216
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 182
DP  - 2023 Dec
TI  - Combined exposure to multiwalled carbon nanotubes and dibutyl phthalates 
      aggravated airway inflammation in rats.
PG  - 114188
LID - S0278-6915(23)00590-2 [pii]
LID - 10.1016/j.fct.2023.114188 [doi]
AB  - Previous work has shown that mice exposed to dibutyl phthalate (DBP) adsorbed 
      onto multi-walled carbon nanotubes (MWCNTs), via tail vein injection, displayed 
      black lesions in their lungs. To investigate the mechanism causing this toxicity 
      in the lung tissue, we performed an experiment with rats, exposing them to DBP 
      adsorbed onto MWCNTs via a tail vein injection for 14 days. The results revealed 
      pulmonary edema and greyish-black lung tissue in the MWCNTs and the MWCNTs + DBP 
      combined exposure groups. In the combined exposure group there was evident 
      alveolar fragmentation and adhesion, and lung tissue sections showed significant 
      levels of black particles. Sections of the non-cartilaginous region of the 
      trachea had significant folding of the pseudostratified ciliated columnar 
      epithelium and marked thickening of the submucosa. In broncho alveolar lavage 
      fluid, the number of leukocytes (WBC), lymphocytes (Lym), neutrophils (Neu), and 
      eosinophils (Eos), as well as levels of immunoglobulin E (IgE), interleukin 6 
      (IL-6), tumor necrosis factor alpha (TNF-alpha), and interleukin 1beta (IL-1beta) were all 
      significantly higher. TNF-alpha, IL-6, signal transducer and activator of 
      transcription 3 (STAT3), and alpha-smooth muscle actin (alpha-SMA) mRNA expression were 
      all elevated in the lung tissue. The combined exposure group, which had 
      considerable airway remodeling, had a greater degree of tracheal constriction and 
      luminal narrowing, according to the results of the alpha-SMA immunofluorescence 
      assay. According to these experimental findings, the exposure to both MWCNTs and 
      DBP seemed to have a synergistic effect and exacerbated rats' impaired 
      respiratory function that resulted from exposure to MWCNTs alone.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - He, Suli
AU  - He S
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China.
FAU - Peng, Haiyan
AU  - Peng H
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China.
FAU - Wu, Min
AU  - Wu M
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China.
FAU - Yan, Chao
AU  - Yan C
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China.
FAU - Wan, Jian
AU  - Wan J
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China.
FAU - Ye, Xin
AU  - Ye X
AD  - Liquor Marking Biological Technology and Application of Key Laboratory of Sichuan 
      Province, College of Bioengineering, Sichuan University of Science & Engineering, 
      Yibin, 644000, China.
FAU - Zhang, Hongmao
AU  - Zhang H
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China.
FAU - Ding, Shumao
AU  - Ding S
AD  - Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of 
      Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China. 
      Electronic address: dingsm@ccnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231113
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Nanotubes, Carbon)
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Rats
MH  - Mice
MH  - Animals
MH  - *Nanotubes, Carbon/toxicity
MH  - Dibutyl Phthalate/toxicity/metabolism
MH  - Cytokines/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Interleukin-6/metabolism
MH  - Lung
MH  - Inflammation/metabolism
OTO - NOTNLM
OT  - Airway inflammation
OT  - Asthma
OT  - Combined exposure
OT  - Dibutyl phthalate
OT  - Multi-walled carbon nanotubes
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/11/16 00:42
MHDA- 2023/12/17 09:45
CRDT- 2023/11/15 19:24
PHST- 2023/09/01 00:00 [received]
PHST- 2023/10/25 00:00 [revised]
PHST- 2023/11/08 00:00 [accepted]
PHST- 2023/12/17 09:45 [medline]
PHST- 2023/11/16 00:42 [pubmed]
PHST- 2023/11/15 19:24 [entrez]
AID - S0278-6915(23)00590-2 [pii]
AID - 10.1016/j.fct.2023.114188 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2023 Dec;182:114188. doi: 10.1016/j.fct.2023.114188. Epub 2023 
      Nov 13.