PMID- 37968819
OWN - NLM
STAT- MEDLINE
DCOM- 20231117
LR  - 20231119
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 41
IP  - 11
DP  - 2023 Nov
TI  - [Determination of seven mycotoxins in cereals by ultra performance liquid 
      chromatography-quadrupole-time of flight mass spectrometry based on the 
      self-built database].
PG  - 1002-1009
LID - 10.3724/SP.J.1123.2023.07014 [doi]
AB  - A method for the determination of seven mycotoxins in rice and wheat by ultra 
      performance liquid chromatography-quadrupole-time of flight mass spectrometry 
      (UPLC-Q-TOF/MS) based on self-built database was established. Samples were 
      extracted with 0.2% formic acid aqueous solution-acetonitrile (50ratio50, v/v), 
      dehydrated and salted according to the QuEChERS method (4 g of magnesium sulfate, 
      1 g of sodium chloride, 1 g of sodium citrate, 0.5 g of citrate disodium salt), 
      and separated on an HSS T3 column (100 mmx2.1 mm, 1.8 mum). UPLC-Q-TOF/MS with 
      MS(E) screening was performed, and the positive and negative ions of the screened 
      mycotoxins were calibrated and quantified using matrix-matched standard curves 
      with time of flight multiple reaction monitoring (TOF-MRM). The results showed 
      that aflatoxin G(1) (AFG(1)), aflatoxin G(2) (AFG(2)), aflatoxin B(1) (AFB(1)), 
      aflatoxin B(2) (AFB(2)), and ochratoxin A (OTA) exhibited moderate matrix effects 
      in rice, while OTA and zearalenone (ZEN) exhibited moderate matrix effects in 
      wheat. The seven mycotoxins showed good linearities in their respective 
      concentration ranges, with correlation coefficients (r) of 0.9900-0.9998. The 
      limits of detection (LODs) for rice and wheat were 0.50-400 and 0.50-200 mug/kg, 
      respectively, and the limits of quantification (LOQs) for both cereals were 
      1.00-800 mug/kg. In rice, the average recoveries at three spiked levels of low, 
      medium, and high were 88.1%-123.9%, with relative standard deviations (RSDs) of 
      0.2%-13.6%. In wheat, the average recoveries at three spiked levels of low, 
      medium, and high were 102.0%-123.4%, with RSDs of 0.8%-14.8%. As a result, one 
      batch of 46 batches of rice was screened out for AFB(1) and AFB(2), with a 
      screening rate of 2.2%, of which the measured values were 10.8 mug/kg and 1.2 
      mug/kg, respectively. According to GB 2761-2017, the maximum allowable level of 
      AFB(1) in rice is 10 mug/kg; thus, the exceeding rate for AFB(1) is 2.2%. 
      Deoxynivalenol (DON) was screened out in 9 out of 24 batches of wheat (screening 
      rate, 37.5%), while ZEN was screened out in 19 batches (screening rate, 79.2%). 
      According to GB 2761-2017, the maximum allowable levels of DON and ZEN in wheat 
      are 1000 and 60 mug/kg, respectively. The levels of DON and ZEN detected in the 
      wheat samples did not exceed these limits. The proposed method uses MS(E) for 
      qualitative screening to avoid the occurrence of false positives caused by 
      interfering compounds with mass numbers and retention times similar to those of 
      the analytes. TOF-MRM mode is then used to quantify the positively screened 
      mycotoxins. The method is fast, accurate, sensitive, and suitable for the 
      isolation and quantitative detection of mycotoxin residues in rice and wheat 
      samples. The findings provide powerful technical support for mycotoxin 
      contamination monitoring in rice and wheat and early risk-warning efforts.
FAU - Zhang, Lu-Xing
AU  - Zhang LX
AD  - Zhejiang Pharmaceutical University, Ningbo 315100, China.
FAU - Zhou, Zheng
AU  - Zhou Z
AD  - Ningbo Institute for Drug Control, Ningbo 315048, China.
FAU - Cao, Lin
AU  - Cao L
AD  - Ningbo Institute for Drug Control, Ningbo 315048, China.
FAU - Qian, Jiang
AU  - Qian J
AD  - Zhejiang Pharmaceutical University, Ningbo 315100, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Mycotoxins)
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - *Mycotoxins/analysis
MH  - Edible Grain/chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Tandem Mass Spectrometry/methods
MH  - Chromatography, Liquid
MH  - *Zearalenone/analysis
PMC - PMC10654887
OTO - NOTNLM
OT  - QuEChERS
OT  - mycotoxins
OT  - rice
OT  - ultra performance liquid chromatography-quadrupole-time of flight mass 
      spectrometry (UPLC-Q-TOF/MS)
OT  - wheat
EDAT- 2023/11/16 06:44
MHDA- 2023/11/17 15:27
PMCR- 2023/11/08
CRDT- 2023/11/16 00:29
PHST- 2023/11/17 15:27 [medline]
PHST- 2023/11/16 06:44 [pubmed]
PHST- 2023/11/16 00:29 [entrez]
PHST- 2023/11/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2023.07014 [doi]
PST - ppublish
SO  - Se Pu. 2023 Nov;41(11):1002-1009. doi: 10.3724/SP.J.1123.2023.07014.