PMID- 37972286 OWN - NLM STAT- MEDLINE DCOM- 20240304 LR - 20240304 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) VI - 13 IP - 6 DP - 2024 Mar TI - Microfluidic Encapsulation of Exosomes Derived from Lipopolysaccharide-Treated Mesenchymal Stem Cells in Hyaluronic Acid Methacryloyl to Restore Ovarian Function in Mice. PG - e2303068 LID - 10.1002/adhm.202303068 [doi] AB - Premature ovarian failure (POF) features an upward incidence nowadays, and the human umbilical cord mesenchymal stem cells (hUC-MSCs)-derived exosomes (MSC-Exos) have shown applied values in the recovery of ovarian function. Here, a novel exosome-encapsulated microcarrier prepared by microfluidic technology for ovarian repair after chemotherapy damage is presented. The exosomes derived from lipopolysaccharide (LPS)-preconditioned hUC-MSCs are encapsulated with hyaluronic acid methacryloyl (HAMA) via microfluidic electrospray, which is named HAMA/MSC-Exos. Attributing to the biocompatibility and semipermeable property of HAMA, the encapsulated exosomes show great viability and controllable release behavior from HAMA. It is demonstrated that in situ transplantation of HAMA/MSC-Exos can rescue ovarian functions of cyclophosphamide-induced ovarian failure in mice by increasing ovarian volume, improving the number of antral follicles and restoring fertility. It is believed that the transplantation of HAMA/MSC-Exos will provide a new concept for the treatment of POF in clinical practice. CI - (c) 2023 Wiley-VCH GmbH. FAU - Li, Yifan AU - Li Y AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, 210008, China. FAU - Zhang, Hui AU - Zhang H AD - School of Life Science and Technology, Southeast University, Nanjing, 210000, China. FAU - Cai, Changjun AU - Cai C AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, 210008, China. FAU - Mao, Jialian AU - Mao J AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210008, China. FAU - Li, Ning AU - Li N AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210008, China. FAU - Huang, Danqing AU - Huang D AD - Institute of Translational Medicine, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210002, China. FAU - Li, Shiyuan AU - Li S AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, 210008, China. AD - Department of Vascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. FAU - Yang, Jun AU - Yang J AD - Department of Pathology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. FAU - Zhou, Jidong AU - Zhou J AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, 210008, China. FAU - Wang, Huan AU - Wang H AD - The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen, 518033, China. FAU - Zhu, Yujuan AU - Zhu Y AD - Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, China. FAU - Ding, Lijun AU - Ding L AUID- ORCID: 0000-0002-6330-7945 AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, 210008, China. AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Clinical College of Nanjing University of Chinese Medicine, Nanjing, 210008, China. AD - Center for Clinical Stem Cell Research, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - State Key Laboratory of Analytic Chemistry for Life Science, Nanjing University, Nanjing, 210093, China. FAU - Sun, Haixiang AU - Sun H AD - Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China. AD - Center for Molecular Reproductive Medicine, Nanjing University, Nanjing, 210008, China. LA - eng GR - 82271671/Innovative Research Group Project of the National Natural Science Foundation of China/ GR - 2018YFC1004701/National Key Research and Development Program of China/ PT - Journal Article DEP - 20231116 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 RN - 9004-61-9 (Hyaluronic Acid) RN - 0 (Lipopolysaccharides) SB - IM MH - Humans MH - Animals MH - Mice MH - *Exosomes MH - Hyaluronic Acid/pharmacology MH - Lipopolysaccharides/pharmacology MH - Microfluidics MH - *Mesenchymal Stem Cells OTO - NOTNLM OT - exosomes OT - human umbilical cord mesenchymal stem cells OT - hyaluronic acid methacryloyl OT - microfluidics OT - premature ovarian failure EDAT- 2023/11/17 15:23 MHDA- 2024/03/04 06:50 CRDT- 2023/11/16 15:03 PHST- 2023/10/25 00:00 [revised] PHST- 2023/09/13 00:00 [received] PHST- 2024/03/04 06:50 [medline] PHST- 2023/11/17 15:23 [pubmed] PHST- 2023/11/16 15:03 [entrez] AID - 10.1002/adhm.202303068 [doi] PST - ppublish SO - Adv Healthc Mater. 2024 Mar;13(6):e2303068. doi: 10.1002/adhm.202303068. Epub 2023 Nov 16.