PMID- 37980546
OWN - NLM
STAT- MEDLINE
DCOM- 20231120
LR  - 20231120
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 39
IP  - 11
DP  - 2023 Number
TI  - [Cheng's Juanbi Decoction alleviates the inflammation in collagen-induced 
      arthritis (CIA) rats via inhibiting activation of PI3K/AKT/mTOR pathway].
PG  - 961-966
AB  - Objective To investigate the potential mechanism of Cheng's Juanbi Decoction 
      (JBT) for treating collagen-induced arthritis (CIA) in rats. Methods Female SD 
      rats were divided into normal group, CIA model group, methotrexate (MTX) group, 
      JBT group with different doses, and LY294002 (PI3K blocker) group. The effects of 
      JBT on toe swelling and arthritis index of rats before and after treatment were 
      evaluated. HE staining was used to observe the pathological changes of synovial 
      tissues. ELISA was used to determine the levels of interleukin-1beta (IL-1beta) and 
      tumor necrosis factor alpha(TNF-alpha) in synovium of rats. Real-time quantitative PCR 
      was used to detect mRNA expression levels of phosphatidylinositol 3 kinase 
      (PI3K), protein kinase B (AKT), mammalian target of rapamycin (mTOR), beclin-1, 
      and P62. The expressions of AKT, phosphorylated AKT (p-AKT), mTOR, phosphorylated 
      mTOR (p-mTOR), PI3K, phosphorylated PI3K (p-PI3K), P62, beclin-1, and 
      microtubule-associated protein 1 light chain 3B (LC3B) were detected by Western 
      blot analysis. Results Compared with the normal group, the toe of other groups 
      was significantly swollen 1 hour before administration. Compared with the 
      conditions 1 hour before administration, toe swelling in the high-dose JBT group, 
      MTX group, and LY294002 group was significantly relieved 2 hours before blood 
      collection after 30 days of administration. JBT can significantly reduce the 
      degree of toe swelling, arthritis index(AI) score, and the destruction of 
      synovial tissue. The levels of IL-1beta, TNF-alpha, mRNA expression of PI3K, AKT, mTOR 
      and P62, and protein levels of p-PI3K, p-AKT, p-mTOR, and P62 in synovium samples 
      of rats in the high-dose JBT group were significantly decreased. Beclin-1 mRNA 
      and protein expression and LC3B protein level were significantly increased. 
      Conclusion JBT may alleviate joint inflammation by inhibiting the activation of 
      the PI3K/AKT/mTOR signaling pathway, and the therapeutic effect of high-dose JBT 
      is comparable to that of MTX and LY294002.
FAU - Sun, Guanghan
AU  - Sun G
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
FAU - Zhu, Jun
AU  - Zhu J
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
FAU - Xu, Xia
AU  - Xu X
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China. *Corresponding author, E-mail: 907367552@qq.com.
FAU - Wan, Lei
AU  - Wan L
AD  - The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 
      230031, China.
FAU - Nan, Shuling
AU  - Nan S
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
FAU - Wang, Yufeng
AU  - Wang Y
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
FAU - Zhao, Li
AU  - Zhao L
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
FAU - Cheng, Hui
AU  - Cheng H
AD  - Scientific Research and Technology Center of Anhui University of Chinese 
      Medicine, Hefei 230021, China.
FAU - Wang, Kun
AU  - Wang K
AD  - Scientific Research and Technology Center of Anhui University of Chinese 
      Medicine, Hefei 230021, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
FAU - Ouyang, Zeng
AU  - Ouyang Z
AD  - School of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - W36ZG6FT64 (Sirolimus)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Beclin-1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.1 (mTOR protein, rat)
SB  - IM
MH  - Rats
MH  - Female
MH  - Animals
MH  - *Proto-Oncogene Proteins c-akt/metabolism
MH  - Phosphatidylinositol 3-Kinase/metabolism/therapeutic use
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - *Arthritis, Experimental/drug therapy/metabolism
MH  - Sirolimus/therapeutic use
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Rats, Sprague-Dawley
MH  - Beclin-1/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Inflammation/drug therapy
MH  - RNA, Messenger/metabolism
MH  - Mammals/metabolism
EDAT- 2023/11/19 09:42
MHDA- 2023/11/20 06:55
CRDT- 2023/11/19 04:12
PHST- 2023/11/20 06:55 [medline]
PHST- 2023/11/19 09:42 [pubmed]
PHST- 2023/11/19 04:12 [entrez]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Number;39(11):961-966.