PMID- 37980746
OWN - NLM
STAT- MEDLINE
DCOM- 20240108
LR  - 20240108
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 103
IP  - 1
DP  - 2024 Jan
TI  - Resveratrol alleviates heat-stress-induced impairment of the jejunal mucosa 
      through TLR4/MAPK signaling pathway in black-boned chicken.
PG  - 103242
LID - S0032-5791(23)00761-7 [pii]
LID - 10.1016/j.psj.2023.103242 [doi]
LID - 103242
AB  - Heat stress in chickens caused by high temperatures in summer is a serious issue 
      faced by the poultry industry globally, which reduces product quality. The aim of 
      this study is to investigate the role of resveratrol in alleviating heat stress 
      injury and inflammatory response of jejunal mucosa in black-boned chickens 
      through TLR4/MAPK signaling pathway. In total, 240 black-boned chickens (28-day 
      old) were randomly divided into 4 treatment groups as follows. The normal 
      temperature (NT) and normal temperature with resveratrol (NT+Res) groups received 
      a basal diet without and with 400 mg/kg resveratrol, respectively, and treated at 
      24℃ +/- 2℃, 24 h/d. The high temperature (HT) and high temperature with resveratrol 
      (HT+Res) groups received basal diet without and with 400 mg/kg resveratrol, 
      respectively, and treated at 37℃ +/- 2℃ for 8 h/d and 24 degrees C +/- 2 degrees C for the rest of 
      the time for 12 d. The results revealed the heat-stress responses impaired the 
      villous structure of the jejunum, causing a rough and uneven surface of the 
      jejunal villus, and local intestinal villi were even more prone to rupture. 
      However, resveratrol significantly improved the morphology and structure of 
      jejunal mucosa under heat stress. Heat stress increased the mRNA levels of 
      toll-like receptor 4 (TLR4), c-Jun, c-fos, caspase-3, and p38 (P < 0.05), reduced 
      mRNA level of Bcl-2, and reduced the expression of tight junction proteins 
      Occludin, ZO-1, and Claudin1 (P < 0.05) in the jejunal mucosa. However, 
      resveratrol inhibited the TLR4/ mitogen-activated protein kinase (MAPK) signaling 
      pathway via downregulating TLR4, c-Jun, p38, and caspase-3 (P < 0.05); 
      upregulating Bcl-2 (P < 0.05); decreasing the protein levels of MKK3, p53, and 
      myeloid differentiation factor 88 (MYD88); and increasing the protein levels of 
      Occludin, ZO-1, and Claudin1. In addition, it reduced the levels of JNK and p38 
      proteins (P < 0.05) and inflammatory factors like tumor necrosis factor-alpha (TNF-alpha) 
      in the jejunal mucosa of black-boned chickens under heat stress. In conclusion, 
      resveratrol may play a regulatory role in heat-stress-induced damage and 
      inflammatory response in the intestinal mucosa of black-boned chickens under heat 
      stress.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Liu, Lili
AU  - Liu L
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China. Electronic address: liulili276@163.com.
FAU - Gong, Xiaoyi
AU  - Gong X
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
FAU - Zhang, Xinyu
AU  - Zhang X
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
FAU - Zhang, Dawei
AU  - Zhang D
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
FAU - Tang, Ying
AU  - Tang Y
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
FAU - Liu, Jiantao
AU  - Liu J
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
FAU - Li, Yajie
AU  - Li Y
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
FAU - Pan, Disheng
AU  - Pan D
AD  - School of Life and Health Sciences, Hunan University of Science and Technology, 
      Xiangtan, Hunan 411201, China.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
DEP - 20231025
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - Q369O8926L (Resveratrol)
RN  - EC 3.4.22.- (Caspase 3)
RN  - 0 (Occludin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (RNA, Messenger)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Animals
MH  - Resveratrol/pharmacology/metabolism
MH  - *Chickens/physiology
MH  - *Jejunum/metabolism
MH  - Caspase 3/metabolism
MH  - Occludin/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Heat-Shock Response
MH  - Signal Transduction
MH  - RNA, Messenger/genetics
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
PMC - PMC10685036
OTO - NOTNLM
OT  - black-boned chicken
OT  - heat stress
OT  - jejunal mucosa
OT  - resveratrol
EDAT- 2023/11/20 00:43
MHDA- 2024/01/08 06:42
PMCR- 2023/10/25
CRDT- 2023/11/19 18:00
PHST- 2023/09/21 00:00 [received]
PHST- 2023/10/23 00:00 [revised]
PHST- 2023/10/23 00:00 [accepted]
PHST- 2024/01/08 06:42 [medline]
PHST- 2023/11/20 00:43 [pubmed]
PHST- 2023/11/19 18:00 [entrez]
PHST- 2023/10/25 00:00 [pmc-release]
AID - S0032-5791(23)00761-7 [pii]
AID - 103242 [pii]
AID - 10.1016/j.psj.2023.103242 [doi]
PST - ppublish
SO  - Poult Sci. 2024 Jan;103(1):103242. doi: 10.1016/j.psj.2023.103242. Epub 2023 Oct 
      25.