PMID- 37981284 OWN - NLM STAT- MEDLINE DCOM- 20240110 LR - 20240110 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 255 DP - 2024 Jan TI - Material extrusion additive manufacturing of poly(lactic acid)/Ti6Al4V@calcium phosphate core-shell nanocomposite scaffolds for bone tissue applications. PG - 128040 LID - S0141-8130(23)04939-5 [pii] LID - 10.1016/j.ijbiomac.2023.128040 [doi] AB - The use of porous scaffolds with appropriate mechanical and biological features for the host tissue is one of the challenges in repairing critical-size bone defects. With today's three-dimensional (3D) printing technology, scaffolds can be customized and personalized, thereby eliminating the problems associated with conventional methods. In this work, after preparing Ti6Al4V/Calcium phosphate (Ti64@CaP) core-shell nanocomposite via a solution-based process, by taking advantage of fused deposition modeling (FDM), porous poly(lactic acid) (PLA)-Ti64@CaP nanocomposite scaffolds were fabricated. Scanning electron microscope (SEM) showed that nanostructured calcium phosphate was distributed uniformly on the surface of Ti64 particles. Also, X-ray diffraction (XRD) indicated that calcium phosphate forms an octacalcium phosphate (OCP) phase. As a result of incorporating 6 wt% Ti64@CaP into the PLA, the compressive modulus and ultimate compressive strength values increased from 1.4 GPa and 29.5 MPa to 2.0 GPa and 53.5 MPa, respectively. Furthermore, the differential scanning calorimetry results revealed an increase in the glass transition temperature of PLA, rising from 57.0 to 62.4 degrees C, due to the addition of 6 wt% Ti64@CaP. However, it is worth noting that there was a moderate decrease in the crystallization and melting temperatures of the nanocomposite filament, which dropped from 97.0 to 89.5 degrees C and 167 to 162.9 degrees C, respectively. The scaffolds were seeded with human adipose tissue-derived mesenchymal stem cells (hADSCs) to investigate their biocompatibility and cell proliferation. Calcium deposition, ALP activity, and bone-related proteins and genes were also used to evaluate the bone differentiation potential of hADSCs. The obtained results showed that introducing Ti64@CaP considerably improved in vitro biocompatibility, facilitating the attachment, differentiation, and proliferation of hADSCs. Considering the findings of this study, the 3D-printed nanocomposite scaffold could be considered a promising candidate for bone tissue engineering applications. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Zarei, Masoud AU - Zarei M AD - Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran; Regenerative Medicine Group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran. FAU - Hasanzadeh Azar, Mahdi AU - Hasanzadeh Azar M AD - Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran. FAU - Sayedain, Sayed Shahab AU - Sayedain SS AD - Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran. FAU - Shabani Dargah, Motahareh AU - Shabani Dargah M AD - Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran. FAU - Alizadeh, Reza AU - Alizadeh R AD - Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran. Electronic address: r.alizadeh@sharif.edu. FAU - Arab, Mehdi AU - Arab M AD - Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran. FAU - Askarinya, Amirhossein AU - Askarinya A AD - Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran. FAU - Kaviani, Alireza AU - Kaviani A AD - Polymeric Materials Research Group (PMRG), Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran. FAU - Beheshtizadeh, Nima AU - Beheshtizadeh N AD - Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Regenerative Medicine Group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran; Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Azami, Mahmoud AU - Azami M AD - Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Regenerative Medicine Group (REMED), Universal Scientific Education and Research Network (USERN), Tehran, Iran; Joint Reconstruction Research Center (JRRC), Tehran University of Medical Sciences, Tehran, Iran. Electronic address: m-azami@tums.ac.ir. LA - eng PT - Journal Article DEP - 20231120 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 459TN2L5F5 (poly(lactide)) RN - 12743-70-3 (titanium alloy (TiAl6V4)) RN - 0 (Polyesters) RN - 0 (Calcium Phosphates) SB - IM MH - Humans MH - *Tissue Scaffolds/chemistry MH - Bone and Bones MH - Polyesters/chemistry MH - Tissue Engineering/methods MH - *Nanocomposites/chemistry MH - Calcium Phosphates/chemistry MH - Printing, Three-Dimensional MH - Porosity OTO - NOTNLM OT - Additive manufacturing OT - Bone tissue engineering OT - Calcium phosphate OT - Fused deposition modeling (FDM) OT - Poly(lactic acid)(PLA) OT - Polymer-matrix composites (PMCs) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/11/20 00:43 MHDA- 2024/01/10 06:41 CRDT- 2023/11/19 19:33 PHST- 2023/07/26 00:00 [received] PHST- 2023/10/26 00:00 [revised] PHST- 2023/11/09 00:00 [accepted] PHST- 2024/01/10 06:41 [medline] PHST- 2023/11/20 00:43 [pubmed] PHST- 2023/11/19 19:33 [entrez] AID - S0141-8130(23)04939-5 [pii] AID - 10.1016/j.ijbiomac.2023.128040 [doi] PST - ppublish SO - Int J Biol Macromol. 2024 Jan;255:128040. doi: 10.1016/j.ijbiomac.2023.128040. Epub 2023 Nov 20.