PMID- 37982568
OWN - NLM
STAT- MEDLINE
DCOM- 20240222
LR  - 20240222
IS  - 1533-4058 (Electronic)
IS  - 1533-4058 (Linking)
VI  - 32
IP  - 2
DP  - 2024 Feb 1
TI  - PYCR1, BANF1, and STARD8 Expression in Gastric Carcinoma: A Clinicopathologic, 
      Prognostic, and Immunohistochemical Study.
PG  - 102-110
LID - 10.1097/PAI.0000000000001173 [doi]
AB  - BACKGROUND: It will be important to understand the molecular pathways of gastric 
      cancer (GC) occurrence and progression, thus detecting predictive and prognostic 
      biomarkers of GC. Pyrroline-5-carboxylate reductase 1 (PYCR1) was upregulated in 
      many cancers, suggesting its possible roles in carcinogenesis and tumor 
      metastases. Barrier-of-autointegration factor 1 (BANF1) is a protein family that 
      plays essential roles in maintaining the integrity of an intact cellular genome. 
      Rho-GTPs are molecular switches that control many signal transduction pathways in 
      normal cells, including 3 subgroups from 1 to 3 (DLC1-3). DLC-3, known as 
      StAR-related lipid transfer domain protein 8 (STARD8), and its role in cancers 
      were not sufficiently studied. The study aimed to investigate the significance of 
      PYCR1, BANF1, and STARD8 protein expression in GC tissues and normal gastric 
      mucosa retrieved from patients with GC to detect prognostic roles of expression. 
      PATIENTS AND METHODS: Specimens were collected from 100 patients with gastric 
      carcinoma. After the application of the inclusion criteria of the study, we 
      prepared 100 paraffin blocks from samples of the 100 included patients; each 
      block included samples from gastric carcinoma and adjacent non-neoplastic gastric 
      mucosa. We assessed the expression of PYCR1, BANF1, and STARD8 using 
      immunohistochemistry in all studied samples. We followed patients for the 
      detection of disease progression and survival rates. We correlate PYCR1, BANF1, 
      and STARD8 expression with clinical, pathologic, and prognostic parameters. 
      RESULTS: Overexpression of PYCR1 and BANF1 and decreased expression of STARD8 was 
      found in gastric carcinoma tissues than adjacent non-neoplastic gastric mucosa ( 
      P <0.001), and was positively associated with high grade ( P =0.006), depth of 
      tumor invasion, presence of lymph nodes metastases and advanced stage ( P 
      =0.001), high incidence of GC progression, recurrence, unfavorable disease-free 
      survival ( P =0.003) and unfavorable overall survival rates ( P <0.001). Thus, it 
      was revealed that; in univariate and multivariate analyses, levels of PYCR1, 
      BANF1, and STARD8 are associated with the overall survival rate of GC patients. 
      CONCLUSIONS: We showed that overexpression of PYCR1 and BANF1 and decreased 
      expression of STARD8 in GC tissues was associated with poor prognosis and GC 
      progression.
CI  - Copyright (c) 2023 Wolters Kluwer Health, Inc. All rights reserved.
FAU - Harb, Ola A
AU  - Harb OA
AD  - Departments of Pathology.
FAU - Elfeky, Mariem A
AU  - Elfeky MA
AD  - Departments of Pathology.
FAU - Alabiad, Mohamed Ali
AU  - Alabiad MA
AD  - Departments of Pathology.
FAU - Hemeda, Rehab
AU  - Hemeda R
AD  - Clinical Oncology and Nuclear Medicine.
FAU - Allam, Ahmed S
AU  - Allam AS
AD  - Internal Medicine.
FAU - El Hawary, Amr T
AU  - El Hawary AT
AD  - Internal Medicine.
FAU - Elbaz, Mohammed
AU  - Elbaz M
AD  - Department of Internal Medicine, Faculty of Medicine, Cairo University, Cairo.
FAU - Sharaf, Ahmed L
AU  - Sharaf AL
AD  - Tropical Medicine, Faculty of Medicine, Zagazig University, Zagazig.
FAU - Gertallah, Loay M
AU  - Gertallah LM
AD  - General Surgery.
FAU - Abdelaziz, Ahmed Mesbah
AU  - Abdelaziz AM
AD  - General Surgery.
FAU - Shalaby, Amany Mohamed
AU  - Shalaby AM
AD  - Department of Histology and Cell Biology, Faculty of Medicine, Tanta University, 
      Tanta.
FAU - Alorini, Mohammed
AU  - Alorini M
AD  - Department of Basic Medical Sciences, Unaizah College of Medicine and Medical 
      Sciences, Qassim University, Unaizah.
FAU - Yahia, Amar Ibrahim Omer
AU  - Yahia AIO
AD  - Department of Pathology, College of Medicine, University of Bisha, Bisha, Kingdom 
      of Saudi Arabia.
AD  - Department of Pathology, Faculty of Medicine and Health Sciences, University of 
      Kordofan, Elobeid, Sudan.
FAU - Negm, Mohamed
AU  - Negm M
AD  - General Surgery.
LA  - eng
PT  - Journal Article
DEP - 20231120
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DLC1 protein, human)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 1.5.1.2 (PYCR1 protein, human)
RN  - 0 (STARD8 protein, human)
RN  - 0 (BANF1 protein, human)
SB  - IM
MH  - Humans
MH  - Biomarkers, Tumor/metabolism
MH  - *Carcinoma
MH  - Disease-Free Survival
MH  - GTPase-Activating Proteins
MH  - Prognosis
MH  - *Stomach Neoplasms/metabolism
MH  - Tumor Suppressor Proteins
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/20 13:42
MHDA- 2024/02/20 11:51
CRDT- 2023/11/20 08:24
PHST- 2023/05/20 00:00 [received]
PHST- 2023/10/18 00:00 [accepted]
PHST- 2024/02/20 11:51 [medline]
PHST- 2023/11/20 13:42 [pubmed]
PHST- 2023/11/20 08:24 [entrez]
AID - 00129039-990000000-00148 [pii]
AID - 10.1097/PAI.0000000000001173 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2024 Feb 1;32(2):102-110. doi: 
      10.1097/PAI.0000000000001173. Epub 2023 Nov 20.