PMID- 37987121 OWN - NLM STAT- MEDLINE DCOM- 20240201 LR - 20240425 IS - 1476-4431 (Electronic) IS - 1479-3261 (Print) IS - 1476-4431 (Linking) VI - 34 IP - 1 DP - 2024 Jan-Feb TI - Establishment of a reference interval for thiamine concentrations in healthy dogs and evaluation of the prevalence of absolute thiamine deficiency in critically ill dogs with and without sepsis using high-performance liquid chromatography. PG - 49-56 LID - 10.1111/vec.13341 [doi] AB - OBJECTIVE: To determine the normal reference interval (RI) for thiamine concentrations in healthy dogs and investigate the prevalence of thiamine deficiency in critically ill dogs with and without sepsis. DESIGN: Prospective, observational, multicenter study, conducted between 2019 and 2021. SETTING: Two veterinary university teaching hospitals. ANIMALS: A total of 109 dogs were enrolled into 3 groups: 40 healthy dogs, 33 dogs with suspected or confirmed sepsis and evidence of tissue hypoperfusion (Doppler blood pressure /=3 mmol/L), and 36 dogs with other critical illnesses and evidence of tissue hypoperfusion. INTERVENTIONS: For each dog, CBC, serum biochemistry, plasma lactate concentration, whole-blood thiamine concentration, blood pressure, vital parameters, Acute Patient Physiologic and Laboratory Evaluation (APPLE)(fast) score, and clinical outcomes were recorded, alongside basic patient parameters and dietary history. Whole-blood thiamine pyrophosphate (TPP) concentrations were measured using high-performance liquid chromatography. MEASUREMENTS AND MAIN RESULTS: The RI for whole-blood TPP in healthy dogs was 70.9-135.3 mug/L. Median TPP concentrations were significantly lower in septic dogs compared to healthy controls (P = 0.036). No significant difference in median TPP concentrations was found between septic dogs and nonseptic critically ill dogs, or between healthy dogs and nonseptic critically ill dogs. TPP concentrations were below the normal RI in 27.3% of septic dogs, compared to 19.4% of nonseptic critically ill dogs (P = 0.57). No correlations were found between TPP concentrations and lactate concentrations, age, body condition scores, time since last meal, RBC count, serum alanine aminotransferase, APPLE(fast) scores, or patient outcomes. CONCLUSIONS: TPP concentrations were significantly lower in septic dogs compared to healthy controls, with an absolute thiamine deficiency found in 27.3% of septic dogs. The established TPP RI allows for further investigation of thiamine deficiency in critically ill dogs. CI - (c) Veterinary Emergency and Critical Care Society 2023. FAU - Berlin, Noa AU - Berlin N AUID- ORCID: 0000-0002-9276-5208 AD - Foster Hospital for Small Animals, Cummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts, USA. AD - Center for Resuscitation Science, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA. FAU - Pfaff, Alexandra AU - Pfaff A AUID- ORCID: 0000-0002-6452-356X AD - Foster Hospital for Small Animals, Cummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts, USA. FAU - Rozanski, Elizabeth A AU - Rozanski EA AUID- ORCID: 0000-0003-3233-8930 AD - Foster Hospital for Small Animals, Cummings School of Veterinary Medicine at Tufts University, North Grafton, Massachusetts, USA. FAU - Chalifoux, Nolan V AU - Chalifoux NV AD - Matthew J. Ryan Veterinary Hospital, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Hess, Rebecka S AU - Hess RS AD - Matthew J. Ryan Veterinary Hospital, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Donnino, Michael W AU - Donnino MW AD - Matthew J. Ryan Veterinary Hospital, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Silverstein, Deborah C AU - Silverstein DC AD - Matthew J. Ryan Veterinary Hospital, University of Pennsylvania, Philadelphia, Pennsylvania, USA. LA - eng GR - T32 HL155020/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Multicenter Study DEP - 20231121 PL - United States TA - J Vet Emerg Crit Care (San Antonio) JT - Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001) JID - 101152804 RN - X66NSO3N35 (Thiamine) RN - Q57971654Y (Thiamine Pyrophosphate) RN - 0 (Lactates) SB - IM MH - Humans MH - Dogs MH - Animals MH - Thiamine MH - Prospective Studies MH - Critical Illness MH - Chromatography, High Pressure Liquid/veterinary MH - Prevalence MH - *Thiamine Deficiency/epidemiology/veterinary MH - *Sepsis/epidemiology/veterinary MH - Thiamine Pyrophosphate MH - Lactates MH - *Dog Diseases/epidemiology PMC - PMC11007751 MID - NIHMS1942234 OTO - NOTNLM OT - canine OT - septic shock OT - vitamin B1 COIS- The authors have no disclaimers and no conflict of interest to declare. EDAT- 2023/11/21 06:43 MHDA- 2024/02/01 06:43 PMCR- 2025/01/01 CRDT- 2023/11/21 05:13 PHST- 2022/07/11 00:00 [revised] PHST- 2022/03/25 00:00 [received] PHST- 2022/07/29 00:00 [accepted] PHST- 2025/01/01 00:00 [pmc-release] PHST- 2024/02/01 06:43 [medline] PHST- 2023/11/21 06:43 [pubmed] PHST- 2023/11/21 05:13 [entrez] AID - 10.1111/vec.13341 [doi] PST - ppublish SO - J Vet Emerg Crit Care (San Antonio). 2024 Jan-Feb;34(1):49-56. doi: 10.1111/vec.13341. Epub 2023 Nov 21.