PMID- 37993769
OWN - NLM
STAT- MEDLINE
DCOM- 20231124
LR  - 20231125
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 23
IP  - 1
DP  - 2023 Nov 22
TI  - MicroRNA-21 promotes head and neck squamous cell carcinoma (HNSCC) induced 
      transition of bone marrow mesenchymal stem cells to cancer-associated 
      fibroblasts.
PG  - 1135
LID - 10.1186/s12885-023-11630-7 [doi]
LID - 1135
AB  - BACKGROUND: Most patients diagnosed with head and neck tumor will present with 
      locally advanced disease, requiring multimodality therapy. Bone marrow-derived 
      mesenchymal stromal cells (BMSCs) respond to a variety of tumor cell-derived 
      signals, such as inflammatory cytokines and growth factors. As a result, the 
      inflammatory tumor microenvironment may lead to the recruitment of BMSCs. Whether 
      BMSCs in the tumor environment are more likely to promote tumor growth or tumor 
      suppression is still controversial. We aimed to determine whether 
      microRNA-21(miR-21) would play a vital role in HNSCC induced transition of human 
      bone marrow mesenchymal stem cells (hBMSCs) to cancer-associated fibroblasts 
      (CAFs). METHODS: In this study, we used electron microscope to observed exosomes 
      collected from human tissue and two cell lines. We co-cultured hBMSCs with 
      exosomes from FaDu and Cal-27 cells with miR-21 inhibited or not, then assessed 
      cell cycle changes of hBMSCs with flow cytometry and determined expression level 
      of alpha-SMA and FAP through qRT-PCR and Western blot. RESULTS: We observed an 
      up-regulation of miR-21 expression in HNSCC tissue and FaDu and Cal-27 cells. 
      Importantly, the exosomes derived from both cells induced CAFs-like 
      characteristics in hBMSCs. while treatment with a miR-21 inhibitor effectively 
      suppressed the transition of hBMSCs to CAFs and reversed the changes in the cell 
      cycle distribution. This suggests that miR-21 plays a crucial role in 
      facilitating the transition of hBMSCs to CAFs and modulating the cell cycle 
      dynamics. CONCLUSION: Our findings highlight the significance of miR-21 in 
      mediating the communication between HNSCC cells and hBMSCs through exosomes, 
      leading to the promotion of CAFs-like features and alterations in the cell cycle 
      of hBMSCs.
CI  - (c) 2023. The Author(s).
FAU - Wang, Hao
AU  - Wang H
AD  - Department of Otorhinolaryngology, Ruijin Hospital Lu Wan Branch, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, 200020, China.
FAU - Zhou, Zhengyu
AU  - Zhou Z
AD  - Department of Laboratory Diagnostics, Changhai Hospital, Naval Medical 
      University, Shanghai, 200433, China.
FAU - Lin, Wenchao
AU  - Lin W
AD  - Department of Otorhinolaryngology, Ruijin Hospital Lu Wan Branch, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, 200020, China.
FAU - Qian, Yechun
AU  - Qian Y
AD  - Department of Otorhinolaryngology, Ruijin Hospital Lu Wan Branch, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, 200020, China.
FAU - He, Shifang
AU  - He S
AD  - Department of Otorhinolaryngology, Ruijin Hospital Lu Wan Branch, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, 200020, China. 
      kingkong0630@126.com.
FAU - Wang, Jun
AU  - Wang J
AD  - Department of Otolaryngology & Head and Neck Surgery, Ruijin Hospital, Shanghai 
      Jiao Tong University School of Medicine, Shanghai, 200020, China. 
      lsqjunjun@126.com.
LA  - eng
GR  - HLM202305/Scientific Research Project of Health and Family Planning Commission of 
      Huangpu District, Shanghai, China/
PT  - Journal Article
DEP - 20231122
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN21 microRNA, human)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/pathology
MH  - *Cancer-Associated Fibroblasts/metabolism
MH  - *Exosomes/genetics/metabolism
MH  - *Head and Neck Neoplasms/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Bone Marrow Cells/metabolism
MH  - Tumor Microenvironment/genetics
PMC - PMC10666302
OTO - NOTNLM
OT  - Cancer-associated fibroblasts
OT  - Exosomes
OT  - Head and neck squamous cell carcinoma
OT  - Human bone marrow mesenchymal stem cells
OT  - MicroRNA-21
COIS- The authors declare no competing interests.
EDAT- 2023/11/23 00:42
MHDA- 2023/11/24 06:42
PMCR- 2023/11/22
CRDT- 2023/11/22 23:55
PHST- 2023/06/04 00:00 [received]
PHST- 2023/11/10 00:00 [accepted]
PHST- 2023/11/24 06:42 [medline]
PHST- 2023/11/23 00:42 [pubmed]
PHST- 2023/11/22 23:55 [entrez]
PHST- 2023/11/22 00:00 [pmc-release]
AID - 10.1186/s12885-023-11630-7 [pii]
AID - 11630 [pii]
AID - 10.1186/s12885-023-11630-7 [doi]
PST - epublish
SO  - BMC Cancer. 2023 Nov 22;23(1):1135. doi: 10.1186/s12885-023-11630-7.