PMID- 37995193
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 4
IP  - 4
DP  - 2023 Dec 15
TI  - Adoptive cell transfer of macrophages following peripheral nerve injury in mice.
PG  - 102624
LID - S2666-1667(23)00591-9 [pii]
LID - 10.1016/j.xpro.2023.102624 [doi]
LID - 102624
AB  - Macrophages are key innate immune cells involved in multiple biological 
      processes, including peripheral nerve regeneration. Here, we describe a protocol 
      for the adoptive cell transfer of bone-marrow-derived macrophages (BMDMs) 
      following sciatic nerve crush injury (SNCI). This procedure involves isolating 
      BMDMs from a donor mouse, potentially manipulating them ex vivo, and 
      reintroducing them into an animal following SNCI. Preclinical studies show that 
      BMDMs can infiltrate injured nerves and impact functional recovery, potentially 
      providing a novel therapy for nerve injuries. For complete details on the use and 
      execution of this protocol, please refer to Jha et al.(1).
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Rawat, Atul
AU  - Rawat A
AD  - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205, USA.
FAU - Jha, Mithilesh Kumar
AU  - Jha MK
AD  - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205, USA.
FAU - Morrison, Brett Michael
AU  - Morrison BM
AD  - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205, USA. Electronic address: bmorris7@jhmi.edu.
LA  - eng
GR  - R01 NS086818/NS/NINDS NIH HHS/United States
GR  - R21 NS130296/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20231122
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Peripheral Nerve Injuries/therapy
MH  - Sciatic Nerve
MH  - Macrophages
MH  - Nerve Regeneration/physiology
MH  - Adoptive Transfer
PMC - PMC10687290
OTO - NOTNLM
OT  - Cell Culture
OT  - Immunology
OT  - Model Organisms
COIS- Declaration of interests The authors declare no competing interests.
EDAT- 2023/11/23 18:41
MHDA- 2024/03/11 06:42
PMCR- 2023/11/22
CRDT- 2023/11/23 12:36
PHST- 2023/05/17 00:00 [received]
PHST- 2023/07/29 00:00 [revised]
PHST- 2023/09/15 00:00 [accepted]
PHST- 2024/03/11 06:42 [medline]
PHST- 2023/11/23 18:41 [pubmed]
PHST- 2023/11/23 12:36 [entrez]
PHST- 2023/11/22 00:00 [pmc-release]
AID - S2666-1667(23)00591-9 [pii]
AID - 102624 [pii]
AID - 10.1016/j.xpro.2023.102624 [doi]
PST - ppublish
SO  - STAR Protoc. 2023 Dec 15;4(4):102624. doi: 10.1016/j.xpro.2023.102624. Epub 2023 
      Nov 22.