PMID- 37995964
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240213
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 339
DP  - 2024 Jan 2
TI  - DUSP1 mRNA modulation during porcine circovirus type 2 and porcine reproductive 
      and respiratory syndrome virus co-infection regulates viruses replication.
PG  - 199282
LID - S0168-1702(23)00244-7 [pii]
LID - 10.1016/j.virusres.2023.199282 [doi]
LID - 199282
AB  - The effects of porcine circovirus type 2b (PCV2b) and porcine reproductive and 
      respiratory syndrome virus (PRRSV) co-infection in epithelial cells of the swine 
      respiratory tract is unknown. In the present study, the newborn pig trachea cell 
      line NPTr-CD163, which is permissive to both viruses, was persistently infected 
      with PCV2b and then with PRRSV. Viral replication, cell viability, cytokines' 
      mRNA expression, and modulation of cellular genes expression were evaluated in 
      infected cells. In NPTr-CD163 co-infection model, PCV2b replication was enhanced 
      while PRRSV replication was suppressed. Cell viability was significantly 
      decreased during PCV2b single infection and co-infection compared to 
      mock-infected and PRRSV single infected cells. However, no difference was 
      observed in cell viability between PCV2b and PCV2b/PRRSV infected cells. The IL6, 
      IL8 and IL10 mRNA expression was significantly higher in co-infected cells 
      compared to PCV2b and PRRSV single infected cells. Moreover, the IFN-alpha/beta 
      expression was significantly reduced in co-infected cells compared to PCV2b 
      infected cells whereas it remained higher compared to PRRSV infected cells. The 
      differential gene expression analysis revealed that the mRNA expression level of 
      the cellular gene DUSP1 was significantly higher in all PRRSV infection models 
      compared to PCV2b single infected cells. Knockdown of DUSP1 expression in 
      co-infected cells significantly reduced PCV2b replication, suggesting a role for 
      DUSP1 in PCV2b/PRRSV pathogenesis.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Burgher-Pulgaron, Yaima
AU  - Burgher-Pulgaron Y
AD  - The Swine and Poultry Infectious Diseases Research Centre (CRIPA-FRQNT), Faculte 
      de Medecine Veterinaire (FMV), Universite de Montreal, 3200 rue Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Provost, Chantale
AU  - Provost C
AD  - Molecular Diagnostic Laboratory, Centre de Diagnostic Veterinaire de l'Universite 
      de Montreal (CDVUM), FMV, Canada.
FAU - Alvarez, Fernando
AU  - Alvarez F
AD  - Infectious Diseases and Immunity in Global Health (IDIGH), McGill University, 
      1001 Decarie, Montreal, Quebec, Canada, H4A 3J1.
FAU - Meza-Serrano, Europa
AU  - Meza-Serrano E
AD  - Centre de Recherche en Reproduction Animale, FMV, Universite de Montreal, Canada.
FAU - Pesant, Marie-Jeanne
AU  - Pesant MJ
AD  - The Swine and Poultry Infectious Diseases Research Centre (CRIPA-FRQNT), Faculte 
      de Medecine Veterinaire (FMV), Universite de Montreal, 3200 rue Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Price, Christopher A
AU  - Price CA
AD  - Centre de Recherche en Reproduction Animale, FMV, Universite de Montreal, Canada.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - The Swine and Poultry Infectious Diseases Research Centre (CRIPA-FRQNT), Faculte 
      de Medecine Veterinaire (FMV), Universite de Montreal, 3200 rue Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2; Molecular Diagnostic Laboratory, Centre de 
      Diagnostic Veterinaire de l'Universite de Montreal (CDVUM), FMV, Canada. 
      Electronic address: carl.a.gagnon@umontreal.ca.
LA  - eng
PT  - Journal Article
DEP - 20231201
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - *Circovirus/genetics
MH  - *Coinfection
MH  - *Swine Diseases
MH  - Virus Replication
MH  - *Porcine Reproductive and Respiratory Syndrome
PMC - PMC10711501
OTO - NOTNLM
OT  - Co-infection
OT  - DUSP1
OT  - NPTr-CD163 cells
OT  - PCV2b
OT  - PRRSV
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/11/24 00:42
MHDA- 2023/12/17 13:19
PMCR- 2023/12/01
CRDT- 2023/11/23 19:31
PHST- 2023/08/02 00:00 [received]
PHST- 2023/11/18 00:00 [revised]
PHST- 2023/11/20 00:00 [accepted]
PHST- 2023/12/17 13:19 [medline]
PHST- 2023/11/24 00:42 [pubmed]
PHST- 2023/11/23 19:31 [entrez]
PHST- 2023/12/01 00:00 [pmc-release]
AID - S0168-1702(23)00244-7 [pii]
AID - 199282 [pii]
AID - 10.1016/j.virusres.2023.199282 [doi]
PST - ppublish
SO  - Virus Res. 2024 Jan 2;339:199282. doi: 10.1016/j.virusres.2023.199282. Epub 2023 
      Dec 1.