PMID- 37996809
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20231127
IS  - 1528-3658 (Electronic)
IS  - 1076-1551 (Print)
IS  - 1076-1551 (Linking)
VI  - 29
IP  - 1
DP  - 2023 Nov 23
TI  - Maresin1 ameliorates MSU crystal-induced inflammation by upregulating Prdx5 
      expression.
PG  - 158
LID - 10.1186/s10020-023-00756-w [doi]
LID - 158
AB  - BACKGROUND: Maresin1 (MaR1) is a potent lipid mediator that exhibits significant 
      anti-inflammatory activity in the context of several inflammatory diseases. A 
      previous study reported that MaR1 could suppress MSU crystal-induced peritonitis 
      in mice. To date, the molecular mechanism by which MaR1 inhibits MSU 
      crystal-induced inflammation remains poorly understood. METHODS: Mousebone 
      marrow-derived macrophages (BMDMs) were pretreated with MaR1 and then stimulated 
      with FAs (palmitic, C16:0 and stearic, C18:0) plus MSU crystals (FAs + MSUc). In 
      vivo, the effects of MaR1 treatment or Prdx5 deficiency on MSUc induced 
      peritonitis and arthritis mouse models were evaluated. RESULTS: The current study 
      indicated that MaR1 effectively suppressed MSUc induced inflammation in vitro and 
      in vivo. MaR1 reversed the decrease in Prdx5 mRNA and protein levels induced by 
      FAs + MSUc. Further assays demonstrated that MaR1 acceleratedPrdx5 expression by 
      regulating the Keap1-Nrf2 signaling axis. Activation of AMPK by Prdx5 improved 
      homeostasis of the TXNIP and TRX proteins and alleviated mitochondrial 
      fragmentation. In addition, Prdx5 overexpression inhibited the expression of 
      CPT1A, a key enzyme for fatty acid oxidation (FAO). Prdx5 protected against 
      defects in FA + MSUc induced FAO and the urea cycle. CONCLUSION: MaR1 treatment 
      effectively attenuated MSUc induced inflammation by upregulating Prdx5 
      expression. Our study provides a new strategy by which Prdx5 may help prevent 
      acute gout attacks.
CI  - (c) 2023. The Author(s).
FAU - Jiang, Hui
AU  - Jiang H
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China.
FAU - Song, DianZe
AU  - Song D
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China.
AD  - Medical Imaging Key Laboratory of Sichuan Province, The Affiliated Hospital of 
      North Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan, 
      China.
FAU - Zhou, Xiaoqin
AU  - Zhou X
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China.
FAU - Chen, Feng
AU  - Chen F
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China.
FAU - Yu, Qingqing
AU  - Yu Q
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China.
AD  - Medical Imaging Key Laboratory of Sichuan Province, The Affiliated Hospital of 
      North Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan, 
      China.
FAU - Ren, Long
AU  - Ren L
AD  - The Fifth People's Hospital of Nanchong City, 21# Bajiao Street, Nanchong, 
      637100, Sichuan, China.
FAU - Dai, Qian
AU  - Dai Q
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China.
FAU - Zeng, Mei
AU  - Zeng M
AUID- ORCID: 0000-0002-5661-7101
AD  - Institute of Rheumatology and Immunology, The Affiliated Hospital of North 
      Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan 
      Province, China. zengmei123@gmail.com.
AD  - Institute of Basic Medicine and Forensic Medicine, North Sichuan Medical College, 
      234# Fujiang Road, Nanchong, 637001, Sichuan Province, China. 
      zengmei123@gmail.com.
AD  - Medical Imaging Key Laboratory of Sichuan Province, The Affiliated Hospital of 
      North Sichuan Medical College, 1# South Maoyuan Road, Nanchong, 637001, Sichuan, 
      China. zengmei123@gmail.com.
AD  - The Fifth People's Hospital of Nanchong City, 21# Bajiao Street, Nanchong, 
      637100, Sichuan, China. zengmei123@gmail.com.
LA  - eng
GR  - 81972119/National Natural Science Foundation of China/
GR  - 22NSFSC0781/Sichuan Province Science and Technology Support Program/
PT  - Journal Article
DEP - 20231123
PL  - England
TA  - Mol Med
JT  - Molecular medicine (Cambridge, Mass.)
JID - 9501023
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 268B43MJ25 (Uric Acid)
RN  - 0 (NF-E2-Related Factor 2)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Kelch-Like ECH-Associated Protein 1/metabolism
MH  - *Uric Acid
MH  - NF-E2-Related Factor 2/metabolism
MH  - Inflammation/metabolism
MH  - *Peritonitis
PMC - PMC10668345
OTO - NOTNLM
OT  - FAO
OT  - Keap1-Nrf2 signaling axis
OT  - MSUc
OT  - MaR1
OT  - Prdx5
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/24 00:42
MHDA- 2023/11/27 12:44
PMCR- 2023/11/23
CRDT- 2023/11/23 23:51
PHST- 2023/06/20 00:00 [received]
PHST- 2023/11/10 00:00 [accepted]
PHST- 2023/11/27 12:44 [medline]
PHST- 2023/11/24 00:42 [pubmed]
PHST- 2023/11/23 23:51 [entrez]
PHST- 2023/11/23 00:00 [pmc-release]
AID - 10.1186/s10020-023-00756-w [pii]
AID - 756 [pii]
AID - 10.1186/s10020-023-00756-w [doi]
PST - epublish
SO  - Mol Med. 2023 Nov 23;29(1):158. doi: 10.1186/s10020-023-00756-w.