PMID- 37998334
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20240210
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 12
IP  - 22
DP  - 2023 Nov 10
TI  - Probiotic and Muscadine Grape Extract Interventions Shift the Gut Microbiome and 
      Improve Metabolic Parameters in Female C57BL/6 Mice.
LID - 10.3390/cells12222599 [doi]
LID - 2599
AB  - Obesity and Western-like diet consumption leads to gut microbiome dysbiosis, 
      which is associated with the development of cardio-metabolic diseases and poor 
      health outcomes. The objective of this study was to reduce Western diet-mediated 
      gut microbial dysbiosis, metabolic dysfunction, and systemic inflammation through 
      the administration of a novel combined intervention strategy (oral probiotic 
      bacteria supplements and muscadine grape extract (MGE)). To do so, adult female 
      C57BL/6 mice were fed a low-fat control or Western-style diet and sub-grouped 
      into diet alone, probiotic intervention, antibiotic treatments, MGE 
      supplementation, a combination of MGE and probiotics, or MGE and antibiotics for 
      13 weeks. Mouse body weight, visceral adipose tissue (VAT), liver, and mammary 
      glands (MG) were weighed at the end of the study. Fecal 16S rRNA sequencing was 
      performed to determine gut bacterial microbiome populations. Collagen, 
      macrophage, and monocyte chemoattractant protein-1 (MCP-1) in the VAT and MG 
      tissue were examined by immunohistochemistry. Adipocyte diameter was measured in 
      VAT. Immunohistochemistry of intestinal segments was used to examine villi 
      length, muscularis thickness, and goblet cell numbers. We show that dietary 
      interventions in Western diet-fed mice modulated % body weight gain, visceral 
      adiposity, MG weight, gut microbial populations, and inflammation. Intervention 
      strategies in both diets effectively reduced VAT and MG fibrosis, VAT and MG 
      macrophages, adipocyte diameter, and VAT and MG MCP-1. Interventions also 
      improved intestinal health parameters. In conclusion, dietary intervention with 
      MGE and probiotics modulates several microbial, inflammatory, and metabolic 
      factors reducing poor health outcomes associated with Western diet intake.
FAU - Newman, Tiffany M
AU  - Newman TM
AD  - Department of Cancer Biology, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
AD  - Department of Surgery-Hypertension, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
FAU - Wilson, Adam S
AU  - Wilson AS
AD  - Department of Surgery-Hypertension, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
FAU - Clear, Kenysha Y J
AU  - Clear KYJ
AD  - Department of Surgery-Hypertension, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
AD  - Department of Physiology and Pharmacology, Wake Forest University School of 
      Medicine, Winston-Salem, NC 27157, USA.
FAU - Tallant, E Ann
AU  - Tallant EA
AD  - Department of Surgery-Hypertension, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
AD  - Comprehensive Cancer Center, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
FAU - Gallagher, Patricia E
AU  - Gallagher PE
AD  - Department of Surgery-Hypertension, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
AD  - Comprehensive Cancer Center, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
FAU - Cook, Katherine L
AU  - Cook KL
AUID- ORCID: 0000-0001-6241-0214
AD  - Department of Cancer Biology, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
AD  - Department of Surgery-Hypertension, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
AD  - Comprehensive Cancer Center, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA.
LA  - eng
GR  - P30 CA012197/CA/NCI NIH HHS/United States
GR  - T32 CA247819/CA/NCI NIH HHS/United States
GR  - T32CA247819/CA/NCI NIH HHS/United States
GR  - P30CA012197/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20231110
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Female
MH  - Animals
MH  - Mice
MH  - *Gastrointestinal Microbiome
MH  - *Vitis
MH  - Dysbiosis/complications
MH  - RNA, Ribosomal, 16S
MH  - Diet, High-Fat
MH  - Mice, Inbred C57BL
MH  - Obesity/metabolism
MH  - *Probiotics/pharmacology
MH  - Inflammation/metabolism
PMC - PMC10670540
OTO - NOTNLM
OT  - Lactobacillus
OT  - diet
OT  - fibrosis
OT  - gut microbiome
OT  - inflammation
OT  - macrophages
OT  - muscadine grape extract
OT  - obesity
OT  - visceral adipose tissue
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/24 12:43
MHDA- 2023/11/27 12:41
PMCR- 2023/11/10
CRDT- 2023/11/24 09:35
PHST- 2023/08/04 00:00 [received]
PHST- 2023/10/19 00:00 [revised]
PHST- 2023/10/27 00:00 [accepted]
PHST- 2023/11/27 12:41 [medline]
PHST- 2023/11/24 12:43 [pubmed]
PHST- 2023/11/24 09:35 [entrez]
PHST- 2023/11/10 00:00 [pmc-release]
AID - cells12222599 [pii]
AID - cells-12-02599 [pii]
AID - 10.3390/cells12222599 [doi]
PST - epublish
SO  - Cells. 2023 Nov 10;12(22):2599. doi: 10.3390/cells12222599.