PMID- 37998893
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231127
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 9
IP  - 11
DP  - 2023 Nov 7
TI  - The Response of Paracoccidioides lutzii to the Interaction with Human 
      Neutrophils.
LID - 10.3390/jof9111088 [doi]
LID - 1088
AB  - The fungal pathogen Paracoccidioides lutzii causes systemic mycosis 
      Paracoccidioidomycosis (PCM), which presents a broad distribution in Latin 
      America. Upon infection, the fungus undergoes a morphological transition to yeast 
      cells and provokes an inflammatory granulomatous reaction with a high number of 
      neutrophils in the lungs. In this work, we employed proteomic analysis to 
      investigate the in vitro response of the fungus to the interaction with human 
      neutrophils. Proteomic profiling of P. lutzii yeast cells harvested at 2 and 4 h 
      post interaction with human polymorphonuclear cells allowed the identification of 
      505 proteins differentially accumulated. The data indicated that P. lutzii yeast 
      cells underwent a shift in metabolism from glycolysis to Beta oxidation, 
      increasing enzymes of the glyoxylate cycle and upregulating enzymes related to 
      the detoxification of oxidative and heat shock stress. To our knowledge, this is 
      the first study employing proteomic analysis in the investigation of the response 
      of a member of the Paracoccidioides genus to the interaction with neutrophils.
FAU - Silva, Lana O'Hara Souza
AU  - Silva LOS
AUID- ORCID: 0000-0002-1655-9576
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania 74690-900, GO, Brazil.
FAU - Baeza, Lilian Cristiane
AU  - Baeza LC
AD  - Laboratorio de Bacteriologia e Micologia Medica, Centro de Ciencias Medicas e 
      Farmaceuticas, Universidade Estadual do Oeste do Parana, Cascavel 85819-110, PR, 
      Brazil.
FAU - Pigosso, Laurine Lacerda
AU  - Pigosso LL
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania 74690-900, GO, Brazil.
FAU - Silva, Kleber Santiago Freitas E
AU  - Silva KSFE
AUID- ORCID: 0000-0001-5350-7313
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania 74690-900, GO, Brazil.
FAU - Pereira, Maristela
AU  - Pereira M
AUID- ORCID: 0000-0002-5482-8036
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania 74690-900, GO, Brazil.
FAU - de Carvalho Junior, Marcos Antonio Batista
AU  - de Carvalho Junior MAB
AUID- ORCID: 0000-0003-1513-3247
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania 74690-900, GO, Brazil.
FAU - de Almeida Soares, Celia Maria
AU  - de Almeida Soares CM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania 74690-900, GO, Brazil.
LA  - eng
GR  - 201810267000022/Fundacao de Apoio a Pesquisa do Estado de Goias/
GR  - 405717/2021-0/National Council for Scientific and Technological Development/
PT  - Journal Article
DEP - 20231107
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC10672145
OTO - NOTNLM
OT  - genus Paracoccidioides
OT  - human neutrophils
OT  - metabolism shunt
OT  - proteomics
OT  - stress response
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2023/11/24 12:42
MHDA- 2023/11/24 12:43
PMCR- 2023/11/07
CRDT- 2023/11/24 09:39
PHST- 2023/09/19 00:00 [received]
PHST- 2023/10/14 00:00 [revised]
PHST- 2023/10/24 00:00 [accepted]
PHST- 2023/11/24 12:43 [medline]
PHST- 2023/11/24 12:42 [pubmed]
PHST- 2023/11/24 09:39 [entrez]
PHST- 2023/11/07 00:00 [pmc-release]
AID - jof9111088 [pii]
AID - jof-09-01088 [pii]
AID - 10.3390/jof9111088 [doi]
PST - epublish
SO  - J Fungi (Basel). 2023 Nov 7;9(11):1088. doi: 10.3390/jof9111088.