PMID- 37998907
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231127
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 9
IP  - 11
DP  - 2023 Nov 12
TI  - Proteomics of Paracoccidioides lutzii: Overview of Changes Triggered by Nitrogen 
      Catabolite Repression.
LID - 10.3390/jof9111102 [doi]
LID - 1102
AB  - Members of the Paracoccidioides complex are the causative agents of 
      Paracoccidioidomycosis (PCM), a human systemic mycosis endemic in Latin America. 
      Upon initial contact with the host, the pathogen needs to uptake micronutrients. 
      Nitrogen is an essential source for biosynthetic pathways. Adaptation to 
      nutritional stress is a key feature of fungi in host tissues. Fungi utilize 
      nitrogen sources through Nitrogen Catabolite Repression (NCR). NCR ensures the 
      scavenging, uptake and catabolism of alternative nitrogen sources, when 
      preferential ones, such as glutamine or ammonium, are unavailable. The 
      NanoUPLC-MS(E) proteomic approach was used to investigate the NCR response of 
      Paracoccidioides lutzii after growth on proline or glutamine as a nitrogen 
      source. A total of 338 differentially expressed proteins were identified. P. 
      lutzii demonstrated that gluconeogenesis, beta-oxidation, glyoxylate cycle, 
      adhesin-like proteins, stress response and cell wall remodeling were triggered in 
      NCR-proline conditions. In addition, within macrophages, yeast cells trained 
      under NCR-proline conditions showed an increased ability to survive. In general, 
      this study allows a comprehensive understanding of the NCR response employed by 
      the fungus to overcome nutritional starvation, which in the human host is 
      represented by nutritional immunity. In turn, the pathogen requires rapid 
      adaptation to the changing microenvironment induced by macrophages to achieve 
      successful infection.
FAU - Cruz-Leite, Vanessa Rafaela Milhomem
AU  - Cruz-Leite VRM
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
FAU - Moreira, Andre Luis Elias
AU  - Moreira ALE
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
FAU - Silva, Lana O'Hara Souza
AU  - Silva LOS
AUID- ORCID: 0000-0002-1655-9576
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
FAU - Inacio, Moises Morais
AU  - Inacio MM
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
AD  - Estacio de Goias University Center-FESGO, Goiania 74063-010, GO, Brazil.
FAU - Parente-Rocha, Juliana Alves
AU  - Parente-Rocha JA
AUID- ORCID: 0000-0002-2666-9701
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
FAU - Ruiz, Orville Hernandez
AU  - Ruiz OH
AD  - MICROBA Research Group, Cellular and Molecular Biology Unit, Department of 
      Microbiology, School of Microbiology, University of Antioquia, Medellin 050010, 
      Colombia.
FAU - Weber, Simone Schneider
AU  - Weber SS
AUID- ORCID: 0000-0002-4181-142X
AD  - Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of 
      Mato Grosso do Sul, Campo Grande 79304-902, MS, Brazil.
FAU - Soares, Celia Maria de Almeida
AU  - Soares CMA
AUID- ORCID: 0000-0001-5378-9840
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
FAU - Borges, Clayton Luiz
AU  - Borges CL
AUID- ORCID: 0000-0002-9914-8771
AD  - Department of Biochemistry and Molecular Biology, Institute of Biological 
      Sciences II, Federal University of Goias, Goiania 74690-900, GO, Brazil.
LA  - eng
GR  - 478990/2012-0 and 408042/2021-4/National Council for Scientific and Technological 
      Development/
GR  - INCT-IPH-FAPEG/Fundacao de Apoio a Pesquisa do Estado de Goias/
GR  - 308237/2022-6/National Council for Scientific and Technological Development/
GR  - DS fellowships/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20231112
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC10672198
OTO - NOTNLM
OT  - NCR-proline
OT  - Paracoccidioides
OT  - metabolic reprogramming
OT  - nitrogen starvation
OT  - proteomics
COIS- The authors declare no conflict of interest.
EDAT- 2023/11/24 12:42
MHDA- 2023/11/24 12:43
PMCR- 2023/11/12
CRDT- 2023/11/24 09:39
PHST- 2023/10/17 00:00 [received]
PHST- 2023/11/06 00:00 [revised]
PHST- 2023/11/08 00:00 [accepted]
PHST- 2023/11/24 12:43 [medline]
PHST- 2023/11/24 12:42 [pubmed]
PHST- 2023/11/24 09:39 [entrez]
PHST- 2023/11/12 00:00 [pmc-release]
AID - jof9111102 [pii]
AID - jof-09-01102 [pii]
AID - 10.3390/jof9111102 [doi]
PST - epublish
SO  - J Fungi (Basel). 2023 Nov 12;9(11):1102. doi: 10.3390/jof9111102.